赵健
发布人:生工学院网站管理员  发布时间:2022-06-15   动态浏览次数:5130


赵健 



生物学专业:博士生导师/硕士生导师

生物工程专业:博士生导师/硕士生导师

生物与医药专业(生物工程领域):博士生导师/硕士生导师


 

赵健,教授、博士:1984年华东化工学院生物化学专业本科毕业;1987-1990年师从中科院上海生物化学研究所孙册研究员,完成硕士论文研究;2004-2009 华东理工大学药学院博士;长期从事生物制药与酶工程研究。

主要主持科研项目: 国家自然科学基金:靶向药物研究;国家十一五支撑计划项目:苦瓜降血糖活性研究;国家“863”项目子课题-生物催化合成芳香手性醇基内酰胺研究以及果蔬洗涤用有机磷水解酶研制;浙江省重大新药创制及合作研发项目等;

主要承担分子生物学,酶学等本科生,研究生课程教学,主编或参编研究生及本科生酶学以及酶工程教材10余部,获研究生、本科生教学成果奖10余次,发表论文50余篇, 申请专利10余项。


科研方向:

1. 靶向抗肿瘤药物的研究与开发

针对不同肿瘤细胞靶标的新型抗肿瘤药物的设计及开发,包括天然药物及基因工程药物。

2. 靶向药物递送载体及其运输途径分子机理研究

  外源性生物活性分子递送工具设计,药物的体内靶向及高效运输机制研究。

3. 酶工程:酶的改造、酶的结构与功能,工业化应用研究

从多种微生物中筛选各种新型药用酶,针对酶的结构改造的应用研究。

 

代表性部分论著:

(1) Chen, Yihui; Li, Si; Zhao, Jian; Cao, Xuewei*; Wang, Fujun; Efficient drug delivery by novel cell-penetrating peptide derived from Midkine, with two heparin binding sites braced by a length-specific helix., Journal of Drug Targeting, 2021, 30(3): 326-333.    

(2) Pang, Guibin; Liu, Yun; Wang, Yangyun; Wang, Yong; Wang, Fujun; Zhao, Jian; Zhang, Leshuai W; Endotoxin contamination in ovalbumin as viewed from a nano-immunotherapy perspective., Wiley Interdiscip Rev Nanomed Nanobiotechnol, 2021.    

(3) Cao, Xue-Wei; Wang, Fu-Jun; Liew, Oi-Wah; Lu, Ye-Zhou; Zhao, Jian; Analysis of Triterpenoid Saponins Reveals Insights into Structural Features Associated with Potent Protein Drug Enhancement Effects., Molecular Pharmaceutics, 2020, 17(2): 683-694.    

(4) Bai, Xue-Di; Cao, Xue-Wei; Chen, Yi-Hui; Fu, Long-Yun; Zhao, Jian*; Wang, Fu-Jun; Constructing a better binding peptide for drug delivery targeting the interleukin-4 receptor, Journal of Drug Targeting, 2020.    

(5) Li, Chen; Cao, Xue-Wei; Zhao, Jian*; Wang, Fu-Jun*; Effective Therapeutic Drug Delivery by GALA3, an Endosomal Escape Peptide with Reduced Hydrophobicity., Journal of Membrane Biology, 2020, 253(2):139-152.    

(6) Wang, Yi-Shuai; Zeng, Xiang-Qing; Yang, Xu-Zhong; Liu, Wei; Li, Peng-Fei; Wang, Fu-Jun; Zhao, Jian*; Real-Time Quantitative PCR Analysis of the Expression Pattern of the Hypoglycemic Polypeptide-P Gene in Momordica charantia, Genes, 2019, 10(12): 1044.    

(7) Zhang, Shulei; Pang, Guibin; Chen, Chao; Qin, Jianzhong; Yu, Huan; Liu, Yongming; Zhang, Xihui; Song, Zhentao; Zhao, Jian; Wang, Fujun*; Wang, Yangyun*; Zhang, Leshuai W.*; Effective cancer immunotherapy by Ganoderma lucidum polysaccharide-gold nanocomposites through dendritic cell activation and memory T cell response, Carbohydrate Polymers, 2019, 205: 192-202.    

(8) Liu, Pei; Lin, Aibo; Zhang, Guoxiu; Zhang, Jiajia; Chen, Yumeng; Shen, Tao; Zhao, Jian; Wei, Dongzhi; Wang, Wei*; Enhancement of cellulase production in Trichoderma reesei RUT-C30 by comparative genomic screening, Microbial Cell Factories, 2019, 18: 81.    

(9) Liu, Pei; Zhang, Guoxiu; Chen, Yumeng; Zhao, Jian; Wang, Wei*; Wei, Dongzhi; Enhanced cellulase production by decreasing intercellular pH through H -ATPase gene deletion in Trichoderma reesei RUT-C30, Biotechnology for Biofuels, 2019, 12(1): 195.    

(10) Xu, Yuan Yuan; Cao, Xue Wei; Fu, Long Yun; Zhang, Tao Zhu; Wang, Fu Jun*; Zhao, Jian*; Screening and characterization of a novel high-efficiency tumor-homing cell-penetrating peptide from the buffalo cathelicidin family, Journal of Peptide Science, 2019, 25(9): UNSP e3201.    

(11) Liu Pei; Wang Wei*; Zhao Jian*; Wei Dongzhi; Screening novel beta-galactosidases from a sequence-based metagenome and characterization of an alkaline beta-galactosidase for the enzymatic synthesis of galactooligosaccharides, Protein Expression and Purification, 2019, 155: 104-111.                  

(12) Song; Zhen-Tao; Zhang; Leshuai W; Fan; Li-Qiang; Kong; Song, Zhen-Tao; Zhang, Leshuai W; Fan, Li-Qiang; Kong, Jing-Wen; Mao, Jun-Hua; Zhao, Jian*; *Wang, Fu-Jun, Enhanced anticancer effect of MAP30-S3 by cyclosproin A through endosomal escape.,Anti-Cancer Drugs, 2018, 29(8): 736-747.

(13) Cao; Xue-Wei; Yang; Xu-Zhong; Du; Xuan; Fu; Long-Yun; Cao, Xue-Wei; Yang, Xu-Zhong;

Du, Xuan; Fu, Long-Yun; Zhang, Tao-Zhu; Shan, Han-Wen; Zhao, Jian*; Wang, Fu-Jun, Structure optimisation to improve the delivery efficiency and cell selectivity of a   tumour-targeting cell-penetrating peptide., Journal of Drug Targeting, 26(9), pp 777-792, 2018-1-16

(14) Song; Zhen-Tao; Zhang; Leshuai W; Fan; Li-Qiang; Kong; Song, Zhen-Tao; Zhang, Leshuai W; Enhanced anticancer effect of MAP30-S3 by cyclosproin A through endosomal escape. Fan, Li-Qiang; Kong, Jing-Wen; Mao, Jun-Hua; Zhao, Jian*;*Wang, Fu-Jun, Anti-Cancer Drugs, 29(8), pp 736-747, 2018-6-14

(15) Lin B, Yang XZ, Cao XW, Zhang TZ, Wang FJ, Zhao, Jian*. A novel trichosanthin fusion protein with increased cytotoxicity to tumor cells., Biotechnol Lett. Jan;39(1):71-78  2016 Oct 6.

(16) Tan MJ, Cao XW, Li PF, Zhai YZ, Zhou Y, Liu YJ, Zhao J*, Wang FJ. Effectively enhancing cytotoxic and apoptotic effects of alpha-momorcharin by integrating a heparin-binding peptide.  Biotechnol Appl Biochem. Nov;64(6):918-926. 2017 Apr 27.

(17) Qiang Lv; Xu-Zhong Yang; Long-Yun Fu; Yv-Ting Lu; Yan-Hua Lu; Zhao J*; Fu-Jun Wang*, Recombinant expression and purification of a MAP30-cell penetrating peptide fusion protein with higher anti-tumor bioactivity, Protein Expression and Purification, 2015:111,9-17

(18) Nguyen, Long The; Yang, Xu-Zhong; Du, Xuan; Wang, Jia-Wei; Zhang, Rui; Zhao J*;   Wang, Fu-Jun*; Dong, Yang; Li, Peng-Fei, Enhancing tumor-specific intracellular delivering efficiency of cell-penetrating peptide by fusion with a peptide targeting to EGFR., Amino Acids, 2015: 47,997–1006.

(19) Zhang, Rui; Yang, Xu-Zhong; Wang, Jia-Wei; Fu, Long-Yun; Zhao J*; Wang, Fu-Jun*, Evaluating the translocation properties of a new nuclear targeted penetrating peptide using two fluorescent markers., J Drug Target, 2015:1, 1-9.

(20) Tian, Miao; Zeng, Xiang-Qing; Song, Huan-Lei; Hu, Shan-Xin; Wang, Fu-Jun*; Zhao J*;   Hu, Zhi-Bi, Molecular diversity and hypoglycemic polypeptide-P content of Momordica charantia in different accessions and different seasons., J Sci Food Agric, 95(6), pp 1328-1335, 2015.

(21) Luo, Xiao-Jing; Kong, Xu-Dong; Zhao J Chen, Qi; Zhou, Jiahai; Xu J-H*. Switching a Newly Discovered Lactonase into an Efficient and Thermostable Phosphotriesterase by Simple Double Mutations His250Ile/Ile263Trp, Biotechnology and Bioengineering, 111(10), pp 1920-1930, 2014/10.

(22). JJ Shangguan, L Fan, X Ju, Q Zhu, FJ Wang, Zhao J*, Xu J-H*. Expression and Characterization of a Novel Enantioselective Lipase from Aspergillus fumigatus. Appl Biochem Biotechnol. 2012;168:1820–1833

(23). Zhao J*, Gao P, Xiao W, Fan LQ, Wang FJ, Li SX, Liu JW. A novel human derived cell-penetrating peptide in drug delivery. Mol Biol Rep. 2011 38:2649–2656

(24). Wang BL, Zhang WJ, Zhao J*, Wang FJ* et al. Gene cloning and expression of a novel hypoglycaemic peptide from Momordica. charantia. J Sci Food Agric. 2011; 91: 2443–2448

(25). Shangguan JJ , Liu YQ, Wang FJ, Zhao J*, Fan LQ, Li SX, Xu J-H*. Expression and characterization of a novel lipase from Aspergillus fumigatus with high specific activity Appl Biochem Biotechnol. Appl Biochem Biotechnol. 2011;165:949–962

(26). Wang Y, Zhao J*, Xu JH*, Fan LQ, Li SX, Zhao LL, Mao XB. Significantly Improved Expression and Biochemical Properties of ecombinant Serratia marcescens Lipase as Robust Biocatalyst for  Kinetic Resolution of Chiral Ester. Appl Biochem Biotechnol, 2010 162(8):2387-99.


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