蔡孟浩
发布人:生工学院网站管理员  发布时间:2022-06-17   动态浏览次数:13067


蔡孟浩



生物化工专业:博士生导师/硕士生导师

生物工程专业:博士生导师/硕士生导师

生物与医药专业(生物工程领域):工程博士生导师/工程硕士生导师




 


男,博士,副教授,博士生导师,生物反应器工程国家重点实验室PI。获(入选)国家高层次人才特殊支持计划(2021)、上海市青年科技启明星计划A(2019),华东理工大学青年英才培育计划A(2019)等资助。入选教育部首批高校支部书记双带头人培训示范班(2018),上海高校双带头人教师党支部书记工作室(2019)。担任中国微生物学会海洋分会专委会委员,中国生物化学与分子生物学学会海洋分会理事,上海市生物工程学会合成生物学专业委员会委员,中国药学会海洋药物专委会青年委员会委员,并担任三家企业的科学家委员会委员。

2006年毕业于华东理工大学理工优秀生部,获生物工程学士学位。2006-2011年中英联合培养博士生,获得生物化工博士学位并留校任教。2014年担任副教授/硕导,2019年破格提为博导。作为项目负责人,主持国家重点研发计划课题、国家自然科学基金项目、国家重大新药创制合作课题、国家863课题、上海科技创新行动计划项目、中央高校基本科研业务费基金、企业横向合作项目等15项,完成专利转让500万元。在Science AdvancesPNASMetabolic EngineeringJournal of Biology ChemistryBiotechnology and BioengineeringACS Synthetic Biology等著名期刊发表论文70余篇,申请/公开中国发明专利23(已授权15)。担任Bioresources and Bioprocessing青年编委、Yeast专刊编委等,Nature CatalysisNature SynthesisNature communications20多个期刊审稿人。主讲本科生微生物学、抗体工程课程,硕士生基因工程制药课程,参讲本科生分子生物学(英文)、博士生微生物细胞信号转导(英文)课程。

研究方向:

主要研究方向为微生物合成生物学,旨在通过新生物元件挖掘与遗传工具设计、新型底盘宿主改造与重塑、代谢途径重构与发酵优化调控,实现不同药/食源功能产物的高效发酵生产,解决其来源受限、制备困难、成本高等问题。

() 微生物底盘创制与药物生物合成:聚焦医药、食品安全菌株毕赤酵母,设计开发新型酵母底盘系统。解析醇氧化酶启动子转录调控机制,设计特色生物元器件并重构代谢模式,开发多样化(如无甲醇诱导、低甲醇强诱导、乙醇强诱导型等)高版本底盘系统,促进多碳源体系的高值化利用,实现重组蛋白药物、小分子天然药物、新型食品替代蛋白等功能分子的低成本、高效表达。

() 药食源微生物底盘发酵调控与优化:依据微生物生理代谢特性,设计个性化发酵调控策略,优化产物途径模块的分配组装,开发定量化工程菌菌群共培养发酵策略,建立酵母菌、丝状真菌的发酵新工艺,实现目标分子产量提升1100多倍,实现从实验室小试优化到工厂中试制备的一体化体系,加速推进候选药物分子的临床前及临床研究。

承担项目:

1.  2022-2025,双醇协同利用型新一代甲醇酵母细胞工厂创制与应用(国家特殊支持人才计划项目,负责人)

2.  2022-2025,产红色素南极真菌的适冷关键基因挖掘及常温适应遗传改造(国家自然科学基金项目,负责人)

3.  2022-2023,毕赤酵母基因编辑表达重组蛋白的技术优化(企业合作项目,负责人)

4.  2022-2022,新一代甲醇酵母细胞工厂创制应用(华东理工大学优秀人才培养基金,负责人)

5.  2020-2020,酵母菌群设计与应用(华东理工大学杰出人才培养基金,负责人)

6.  2019-2022,甲醇同化非交互菌群设计与药物合成应用(上海市青年科技启明星人才项目,负责人)

7.  2019-2022,甲醇酵母正交/交互生物器件库设计与精细表达控制(国家自然科学基金项目,负责人)

8.  2018-2021,黄芩素等黄酮的合成生物学制备体系研建(国家重点研发计划课题,子课题负责人)

9. 2018-2021,极地微生物资源开发的发酵工艺研究(国家重点研发计划课题,负责人)

10. 2017-2018,大肠杆菌发酵工艺开发(企业合作项目-细胞治疗质粒规模化制备,负责人)

11. 2017-2020,甲醇酵母cPAOX1-TF(AD)转录调控器件库构建及生物合成系统设计(上海科技创新行动计划基础研究领域项目,负责人)

12. 2017-2019,从基因到分子:深海微生物稀有药源产物的生物合成(生工学院远志计划,负责人)

13. 2015-2017,剪切敏感型霉菌菌丝形成特异性基因发掘及发酵应用(中央高校基本科研业务费专项基金,负责人)

14. 2014-2016,盐度胁迫调控海洋灰绿曲霉发育转变的分子机制研究(国家自然科学基金青年基金,负责人)

15. 2012-2015,极地药源微生物活性产物的规模化发酵与制备(国家863项目子课题,负责人)

16. 2012-2015,海洋抗肿瘤候选药物SZ-685C的发酵优化及制备(国家重大新药专项课题合作课题,负责人)

发表论文(*通讯作者, #共同第一)

1. Liu Q, Song L, Peng Q, Zhu Q, Shi X, Xu M, Wang Q, Zhang Y, Cai M*. A programmable high expression yeast platform responsive to user-defined signals. Science Advances, 2022, 8: eabl5166.

2. Qian Z, Yu J, Chen X, Kang Y, Ren Y, Liu Q, Lu J, Zhao Q*, Cai M*. De novo production of the plant 4’-deoxyflavones baicalein and oroxylin A from ethanol in Crabtree-negative yeast. ACS Synthetic Biology. 2022, 11(4):1600-1612.

3. Ding L, Huang H, Lu F, Lu J, Zhou X, Zhang Y, Cai M*. Transposon insertion mutation of Antarctic psychrotrophic fungus for red pigment production adaptive to normal temperature. J Ind Microbiol Biotechnol. 2021, 0: kuab073.

4. Ren Y, Liu Q, Liu H, Zhou X, Zhang Y, Cai M*. High-level living cell production of cytidine-5’-diphosphocholine in metabolically engineered yeast. J Biotechnol. 2021, 341:129-136.

5. Huang H, Ding L, Lu J, Wang N, Cai M*. Combinatorial strategies for production improvement of red pigments from Antarctic fungus Geomyces sp.. J Food Sci. 2020, 85(10):3061-3071.

6. Bai C, Liu Y, Chen X, Qian Z, Liu H, Zhou X, Zhang Y, Cai M*. Fungal statin pump protein improves monacolin J efflux and regulates its production in Komagataella phaffii. Bioresourc Bioprocess. 2020, 7:32.

7. Wen J, Tian L, Liu Q, Zhang Y,Cai M*. Engineered dynamic distribution of malonyl-CoA flux for improving polyketide biosynthesis in Komagataella phaffii. J Biotechnol. 2020, 320: 80-85

8. Bai C, Liu Y, Chen X, Qian Z, Liu H, Zhou X, Zhang Y, Cai M*. Fungal statin pump protein improves monacolin J efflux and regulates its production in Komagataella phaffii. Bioresourc Bioprocess. 2020, 7:32

9. Wen J, Tian L, Xu M, Zhou X, Zhang Y, Cai M*. A synthetic malonyl-CoA metabolic oscillator in Komagataella phaffii. ACS Syn Biol. 2020, 9:1059-1068

10. Ren Y, Liu Q, Liu H, Zhou X, Zhang Y,Cai M*. Engineering substrate and energy metabolism for living cell production of cytidine-5’-diphosphocholine. Biotechnol Bioeng. 2020, 117(5):1426-1435

11. Liu Q#, Shi X#, Song L, Liu H, Zhou X, Wang Q, Zhang Y, Cai M*. CRISPR-Cas9-mediated genomic multiloci integration in Pichia pastoris. Microb Cell Fact. 2019, 18:144.

12. Liu Y, Bai C, Liu Q, Xu Q, Qian Z, Peng Q, Yu J, Xu M, Zhou X, Zhang Y, Cai M*. Engineered ethanol-driven biosynthetic system for improveing production of acetyl-CoA derived drugs in Crabtree-negative yeast. Metab Eng. 2019, 54: 275-284.

13. Xu Q, Bai C, Liu Y, Song L, Tian L, Yan Y, Zhou J, Zhou X, Zhang Y, Cai M*. Modulation of acetate utilization in Komagataella phaffii by metabolic engineering of tolerance and metabolism. Biotechnol Biofuels. 2019, 12:61.

14. Liu Y, Tu X, Xu Q, Bai C, Kong C, Liu Q, Yu J, Peng Q, Zhou X, Zhang Y, Cai M*. Engineered monoculture and co-culture of methylotrophic yeast for de novo production of monacolin J and lovastatin from methanol. Metab Eng. 2018, 45:189-199.

15. Kong C, Huang H, Xue Y, Liu Y, Peng Q, Liu Q, Yin Y, Zhou X, Zhang Y, Cai M*. Heterologous pathway assembly reveals molecular steps of fungal terreic acid biosynthesis. 2018, Sci Rep. 2018:2116.

16. Wang J, Wang X, Shi L, Zhang Y, Zhou X*, Cai M*. Reduced methanol input induces increased protein output by AOX1 promoter in a transacting elements engineered Pichia pastoris. J Ind Microbiol Biotechnol. 2018, 45:25-30.

17. Shi L, Wang X, Wang J, Zhang P, Qi F, Cai M*, Zhang Y, Zhou X*. Transcriptome analysis of Δmig1Δmig2 mutant reveals their roles in methanol catabolism, peroxisome biogenesis and autophagy in methylotrophic yeast Pichia pastoris. 2018, Gen. Genom. 40:399–412.

18. Liu Y, Bai C, Xu Q, Yu J, Zhou X, Zhang Y, Cai M*. Improved methanolderived lovastatin production through enhancement of the biosynthetic pathway and intracellular lovastatin efflux in methylotrophic yeast. Bioresour. Bioprocess. 2018,5:22

19. Wang J, Wang X, Shi L, Qi F, Zhang P, Zhang Y, Zhou X*, Song Z*, Cai M*. Methanol-independent protein expression by AOX1 promoter with trans-acting elements engineering and glucose-glycerol-shift induction in Pichia pastoris.Sci Rep. 2017, 7:41850.

20. Xue Y, Kong C, Shen W, Bai C, Ren Y, Zhou X, Zhang Y, Cai M*. Methylotrophic yeast Pichia pastoris as a chassis organism for polyketide synthesis via the full citrinin biosynthetic pathway. J Biotechnol. 2017, 242:64-72.

21. Wu Y, Ren Y, Zhou X, Cai M*, Zhang Y*. Transcription factor Agseb1 affects development, osmotic stress response, and secondary metabolism in marine-derived Aspergillus glaucus. J. Basic Microbiol. 2017, 57:873-882 (cover article).

22. Huang M, Gao Y, Zhou X, Zhang Y, Cai M*. Regulating unfolded protein response activator HAC1p for production of thermostable raw-starch hydrolyzing α-amylase in Pichia pastoris. Bioproc. Biosyst. Eng. 2017, 40:341-350.

23. Gao Y, Huang M, Sun X, Zhang X, Zhang Y, Zhou X*, Cai M*. Single-site mutation of C363G or N463T strengthens thermostability improvement of IG181-182 deleted acidic α-amylase from deep-sea thermophile Geobacillus sp. Food Biotechnol. 2017, 31:57-71.

24. Xiao S, Gao Y, Wang X, Shen W, Wang J, Zhou X, Cai M*, Zhang. Peroxisome-targeted and tandem repeat multimer expressions of human antimicrobial peptide LL37 in Pichia pastoris. Prep Biochem Biotechnol. 2017, 47:229-235.

25. Liu S, Li J, Wu Y, Ren Y, Liu Q, Wang Q, Zhou X, Cai M*, Zhang Y. De novo transcriptome sequencing of marine-derived Aspergillus glaucus and comparative analysis of metabolic and developmental variations in response to salt stress. Gen Genom. 2017, 39:317-329.

26. Wang X, Wang Q, Wang J, Bai P, Shi L, Shen W, Zhou M, Zhou X, Zhang Y, Cai M*. Mit1 transcription factor mediates methanol signaling and regulates alcohol oxidase 1 promoter in Pichia pastoris. J Biol Chem. 2016, 291:6245-6261.

27. Zhang X, He H, Yin Y, Zhou W, Cai M*, Zhou X, Zhang Y. A light–dark shift strategy derived from light-responded metabolic behaviors for polyketides production in marine fungus Halorosellinia sp.. J Biotechnol. 2016, 221:34-42.

28. He H, Xue Y, Xu N, Yin Y, Lu J, Cai M*, Zhang Y. A novel pH–precursor–glucose synergic control strategy for dinactin biosynthesis by an arctic actinomycete Streptomyces sp. Process Biochem. 2016, 51:1911-1918.

29. Shen C, Xu N, Gao Y, Sun X, Yin Y*, Cai M*, Zhou X, Zhang Y. Stimulatory effect of ethanol on libertellenone H biosynthesis by Arctic fungus Eutypella sp. D-1. Bioproc. Biosyst. Eng. 2016, 39:353-360.

30. Wang X,Cai M*, Shi L, Wang Q, Zhu J, Wang J, Zhou M, Zhou X, Zhang Y. PpNrg1 is a transcriptional repressor for glucose and glycerol repression of AOX1 promoter in methylotrophic yeast Pichia pastoris. Biotechnol Lett. 2016, 38:291-298.

31. Jiang T, Cai M*, Huang M, He H, Lu J, Zhou X, Zhang Y. Bioprocess exploration for thermostable α-amylase production of a deep-sea thermophile Geobacillus sp. in high temperature bioreactor. Prep Biochem Biotechnol. 2016, 46:620-627.

32. Wang M, He H, Na K, Cai M*, Zhou X, Zhao W, Zhang Y. Designing novel glucose/ornithine replenishment strategies by biosynthetic and bioprocess analysis to improve fibrinolytic FGFC1 production by the marine fungus Stachybotrys longispora. Process Biochem. 2015, 50:2012-2018

33. Jiang T, Cai M*, Huang M, He H, Lu J, Zhou X, Zhang Y. Characterization of a thermostable raw-starch hydrolyzing a-amylase from deep-sea thermophile Geobacillus sp. Protein Expres Purif. 2015, 114:15-22.

34. Zhou J, He H, Wang X, Lu J, Zhou X, Cai M*, Zhang Y. Optimization of nutrients for dinactin production by a marine Streptomyces sp. from the High Latitude Arctic. Biotechnol Bioproc Eng. 2015, 20:725-732.

35. Cai M#, Zhang Y#, Hu W, Shen W, Yu Z, Zhou W, Jiang T, Zhou X*, Zhang Y. Genetically shaping morphology of the filamentous fungus Aspergillus glaucus for production of antitumor polyketide aspergiolide A. Microb Cell Fact. 2014, 13:73.

36. Yu Z, Cai M*, Hu W, Zhang Y, Zhou J, Zhou X, Zhang Y. A cyclin-like protein, ClgA, regulates development in Aspergillus nidulans. Res Microbiol. 2014, 165:462-467.

37. Zhou W, Cai M*, Na K, Shen C, Zhang X, Zhou X, Zhao W, Zhang Y. pH-dependent accumulation of anticancer compound on mycelia in fermentation of marine fungus. J Ind Microbiol Biotechnol. 2014, 41: 1169-1173.

38. Zhou J, Cai M*, Jiang T, Zhou W, Shen W, Zhou X, Zhang Y. Mixed carbon source control strategy for enhancing alginate lyase production by marine Vibrio sp. QY102. Bioproc Biosyst Eng. 2014, 37:575-584.

39. Gao L, Cai M*, Shen W, Xiao S, Zhou X, Zhang Y. Engineered fungal polyketide biosynthesis in Pichia pastoris: a potential excellent host for polyketide production. Microb Cell Fact. 2013, 12:77.

40. Zhou W, Cai M*, Zhou J, Jiang T, Zhou J, Wang M, Zhou X*, Zhang Y. Nutrition and bioprocess development for efcient biosynthesis of an antitumor compound from marine-derived fungus. J Ind Microbiol Biotechnol. 2013, 40:1131-1142.

41. Cai M, Fang Z, Niu C, Zhou X*, Zhang Y. Light regulation on growth, development and secondary metabolism of marine-derived filamentous fungi. Folia Microbiol. 2013, 58: 537-546.

42. Cai M, Zhou X, Lu J, Fan W, Zhou J, Niu C, Kang L, Sun X, Zhang Y. An integrated control strategy for the fermentation of the marine-derived fungus Aspergillus glaucus for the production of anti-cancer polyketide. Mar Biotechnol. 2012, 14:665-671.

43. Cai M, Zhou X*, Sun X, Tao K, Zhang Y*. Statistical optimization of medium composition for aspergiolide A production by marine-derived fungus Aspergillus glaucus. J Ind Microbiol Biotechnol. 2009, 36: 381-389.

44. Cai M, Sun X, Zhou X*, Zhang Y*. Roles of cobalt in biosynthesis stimulation of a cytotoxic compound from marine-derived Aspergillus glaucus. Process Biochem. 2012, 12: 2267–2274.

45. Cai M, Zhou X, Lu J, Fan W, Niu C, Zhou J, Sun X, Kang L, Zhang Y. Enhancing aspergiolide A production from a shear-sensitive and easy-foaming marine-derived filamentous fungus Aspergillus glaucus by oxygen carrier addition and impeller combination in a bioreactor. Bioresourc Technol. 2011, 102: 3584-3586.

46. Cai M, Zhou X, Zhou J, Niu C, Kang L, Sun X, Zhang Y. Efficient strategy for enhancing aspergiolide A production by citrate feedings and its effects on sexual development and growth of marine-derived fungus Aspergillus glaucus. Bioresourc Technol. 2010, 101:6059-6068.

47. Shi L, Wang J, Wang X, Zhang Y, Song Z, Cai M, Zhou X*. Transcriptome and metabolome analyses reveal global behaviour of a genetically engineered methanol-independent Pichia pastoris strain. Process Biochem. 2019, 76:46-54.

48. Zhang X, Gao Y, Yin Y*, Cai M, Zhou X, Zhang Y. Regulation of different polyketide biosynthesis by green light in an endophytic fungus of mangrove leaf. 3 Biotech. 2017, 7:363.

49. Zhang X, Gao Y, Yin Y*, Cai M, Zhou X, Zhang Y. Effective pH pretreatment and cell disruption method for real-time intracellular enzyme activity assay of a marine fungus covered with pigments. Prep Biochem Biotechnol. 2017, 47:211-217.

50. Shen W, Xue Y, Liu Y, Kong C, Wang X, Huang M, Cai M, Zhou X, Zhang Y, Zhou M*. A novel methanol-free Pichia pastoris system for recombinant protein expression. Microb Cell Fact. 2016, 15:178.

51. Shen W, Kong C, Xue Y, Liu Y, Cai M, Zhang Y, Jiang T, Zhou X, Zhou M. Kinase screening in Pichia pastoris identified promising targets involved in cell growth and alcohol oxidase 1 promoter (PAOX1) regulation. PLoS ONE, 2016, 11:e0167766.

52. Yin Y*, Cai M, Zhou X, Li Z, Zhang Y*. Polyketides in Aspergillus terreus: biosynthesis pathway discovery and application. Appl Microbiol Biotechnol. 2016, 100:7787-7798.

53. Wang S, Liu Q*, Cai M, Wang Q, Zhang Y. Fermentation preparation of recombinant Vibrio anguillarum vaccine with heterogeneous antigen display. Prep Biochem Biotechnol. 2013, 43:79-94.

54. Davison J, al Fahad A, Cai M, Song Z, Yehia SY, Lazarus CM, Bailey AM, Simpson TJ, Cox RJ. Genetic, molecular, and biochemical basis of fungal tropolone biosynthesis. Proc Natl Acad Sci U S A. 2012, 109: 7642-7647.

55. Niu C, Cai M, Zhang Y, Zhou X*. Biosynthetic origin of the carbon skeleton of a novel anti-tumor compound, haloroquinone, from a marine-derived fungus, Halorosellinia sp. Biotechnol Lett. 2012, 34:2119-2124.

56. Kang L, Cai M, Yu C, Zhang Y, Zhou X*. Improved production of the anticancer compound 1403C by glucose pulse feeding of marine Halorosellinia sp. (No. 1403) in submerged culture. Bioresourc Technol. 2011, 102:10750-10753.

57. Yu C, Cai M, Kang L, Zhang Y, Zhou X*. Significance of seed culture methods on mycelial morphology and production of a novel anti-cancer anthraquinone by marine mangrove endophytic fungus Halorosellinia sp. (No. 1403). Process Biochem. 2012, 47:422-427.

58. Fang Z, Zhang Y, Cai M, Zhang J, Zhang Y, Zhou X*. Improved gene targeting frequency in marine-derived filamentous fungus Aspergillus glaucus by disrupting ligD. J Appl Genet. 2012, 53:355–362.

59. Tu PG, Wang Y, Cai M, Zhou X*, Zhang Y*. Co-expressions of protein tyrosine kinases EGFR-2 and PDGFRβ with protein tyrosine phosphatase 1B in Pichia pastoris. J Microbiol Biotechnol. 2014, 24: 152-159.

60. Sun X, Zhou X*, Cai M, Tao K, Zhang Y*. Identified biosynthetic pathway of aspergiolide A and a novel strategy to increase its production in a marine-derived fungus Aspergillus glaucus by feeding of biosynthetic precursors and inhibitors simultaneously. Bioresourc Technol. 2009, 100: 4244-4251.

61. Sun X, Zhou X*, Cai M, Zhou J, Zhang Y*. Significant stimulation of o-phthalic acid in biosynthesis of aspergiolide A by a marine fungus Aspergillus glaucus. Bioresourc Technol. 2010, 101: 3609-3616.

62. Tao K, Du L, Sun X, Cai M, Zhu T, Zhou X*, Gu Q, Zhang Y. Biosynthesis of aspergiolide A, a novel antitumor compound by a marine-derived fungus Aspergillus glaucus via the polyketide pathway. Tetra Lett. 2009, 50: 1082-1085.

63. 陈鑫洁, 钱芷兰, 刘启, 赵清, 张元兴, 蔡孟浩*. 毕赤酵母底盘芳香族氨基酸合成途径改造生产肉桂酸及对香豆酸. 2021, 41(10):52-61.

64. 卢凤宁, 张元兴, 蔡孟浩*. 极地酵母菌的发现与应用. 2021, 33(3):315-324.

65. 彭强强, 刘启, 徐名强, 张元兴, 蔡孟浩*. 新型重组毕赤酵母产人胰岛素前体的表达工艺研究. 中国生物工程杂志. 2019, 39(07):48-55.

66. 李佳欣,刘绍梅,任燕娜,周祥山,蔡孟浩*,张元兴. 海洋灰绿曲霉AgveA基因响应盐度胁迫的功能分析. 华东理工大学学报(自然科学版). 2018, 44:650-660.

67. 胡伟,李佳欣,李晓霞,蔡孟浩*,周祥山*,张元兴. 剪切敏感型海洋灰绿曲霉AgugtAAgugeA基因的克隆及功能分析. 华东理工大学学报(自然科学版). 2015, 41: 455-463.

68. 李晓霞, 蔡孟浩*, 胡伟, 李佳欣, 周祥山*, 张元兴. 海洋灰绿曲霉聚酮合酶基因的克隆与序列分析. 华东理工大学学报(自然科学版). 2014, 40: 579-585.

69. 齐飞, 蔡孟浩, 周祥山*, 张元兴. 基于rna-seq技术的毕赤酵母基因组重注释及新型强启动子的发现与序列分析. 华东理工大学学报(自然科学版). 2014, 40:167-175.

70. 柏鹏, 蔡孟浩, 周祥山*, 张元兴. 葡萄糖诱导型AOX1启动子毕赤酵母突变株B5的筛选. 工业微生物2013, 43:69-72.

71. 牛传朋, 蔡孟浩, 李晓霞, 周祥山*, 张元兴. 海洋真菌Xylaria sp.(No.2508)生产xyloketal B的发酵优化.工业微生物 2012, 42:43-47.

72. 张盈, 沈伟, 蔡孟浩, 周祥山*, 张元兴. 海洋真菌灰绿曲霉地标蛋白编码基因AgTeaA的克隆与序列分析. 生物技术通报 2011, 12:157-161.

73. 康丽, 蔡孟浩, 范卫民, 陆健, 周祥山*, 张元兴. 海洋红树林内生真菌产抗肿瘤活性化合物1403C发酵条件的优化. 生物技术通报 2011, 12:199-204.

专著:

1. Qian Z, Liu Q, Cai M*. Investigating fungal biosynthetic pathways using Pichia pastoris as a heterologous host. In: Skellam E. (eds) Engineering Natural Product Biosynthesis. Methods in Molecular Biology, 2022, 2489:115-127. Humana, New York, NY.

2. 蔡孟浩, 周祥山, 张元兴. 海洋微生物活性产物规模化发酵技术. In: 于广利, 谭仁祥主编. 海洋天然产物与药物研究开发. 科学出版社. 2016.

专利:

1. 蔡孟浩, 刘启, 张元兴. 基于CRISPRiCRISPRa的转录调控系统、其建立方法及应用. 申请号202110901314.9.

2. 蔡孟浩, 丁璐璐,卢凤宁. 一种南极嗜冷真菌突变方法及常温适应突变株的筛选. 申请号202110974544.8.

3. 蔡孟浩, 朱巧云, 张元兴. 一种人工转录器件库的构建方法及应用. 申请号202111087812.0.

4. 蔡孟浩, 任燕娜, 张元兴. 一种发酵生产胞磷胆碱的基因工程菌及应用. 申请号202110949063.1.

5. 蔡孟浩, 钱芷兰, 陈鑫洁, 赵清. 生产汉黄芩素类化合物的基因工程酵母菌、其构建方法及应用. 申请号202110918657.6.

6. 蔡孟浩钱芷兰陈鑫洁赵清生产黄芩素类化合物的基因工程酵母菌、其构建方法及应用专利号202010263807.X.

7. 蔡孟浩, 刘启, 师晓娜, 张元兴. 一种用于毕赤酵母表达宿主的多基因敲入方法. 申请号201910403132.1.

8. 蔡孟浩, 刘一奇, 张元兴. 一种莫纳可林J异源生产的方法及应用. 专利号ZL201810857983.9.

9. 蔡孟浩, 任燕娜, 周祥山, 张元兴. 生产胞磷胆碱的基因工程菌的构建与应用.专利号ZL201711180707.5.

10. 蔡孟浩, 刘启, 周祥山, 张元兴. 无碳源阻遏毕赤酵母表达系统、其建立方法及应用.申请号201710352843.1. 公开号108949869A

11. 蔡孟浩, 刘一奇, 张元兴. 生产洛伐他汀及莫纳可林 J 的基因工程菌的构建与应用.专利号ZL201510552643.1.

12. 蔡孟浩, 周久顺, 周祥山, 张元兴. 一种利用海洋弧菌生产褐藻胶裂解酶的方法.专利号ZL201210068851.0.

13. 蔡孟浩, 王美霞, 周祥山, 张元兴. 用于海洋长孢葡萄穗霉生产纤溶活性化合物的培养基.专利号ZL201310170435.6. 

14. 蔡孟浩, 周娇, 周祥山, 张元兴. 用于北极海洋放线菌Streptomyces sp.生产大环四重内酯类化合物的培养基. 专利号ZL201310504941.4.

15. 蔡孟浩, 沈处, 周祥山, 张元兴. 应用于北极丝状真菌生产二萜类化合物的发酵培养基.专利号ZL201510408258.X.

16. 蔡孟浩, 沈处, 周祥山, 张元兴. 添加醇类促进二萜类化合物Libertellenone H产量的方法.专利号ZL201510408062.0.

17. 蔡孟浩, 高燕云, 周祥山, 张元兴. 热稳定性提高的酸性淀粉酶突变体及其制备方法和应用. 专利号ZL201610542505.X.

18. 周祥山, 蔡孟浩, 张元兴, 孙学谦, 陶可敬, 范卫民, 陆健, 顾谦群, 朱天骄. 一种用于海洋灰绿曲霉生产新型抗肿瘤活性化合物的新型培养基及其制备方法. 专利号ZL200810038598.8.

19. 周祥山, 周卫强, 蔡孟浩, 张元兴.利用海洋红树林内生真菌生产抗癌蒽醌类化合物的培养基及制备方法.专利号ZL201210067014.6.

20. 周祥山, 蒋涛, 蔡孟浩, 张元兴. 用于深海海洋嗜热细菌Geobacillus sp.生产淀粉酶的培养基. 专利号ZL201310169378.X.

21. 周祥山, 蒋涛, 蔡孟浩, 张元兴. 用于高密度培养深海海洋嗜热细菌Geobacillus sp.的培养基. 专利号ZL201310170443.0.

22. 周祥山, 周卫强. 蔡孟浩. 张元兴. 一种发酵培养海洋真菌生产抗癌化合物1403C的方法. 专利号ZL201310269533.5.

23. 周祥山, 沈伟, 蔡孟浩, 张元兴.一种褐藻胶裂解酶基因algp及其制备方法与表达. 专利号ZL201410353590.6.

联系方式:

Tel./Fax86-21-64253286

E-mail: cmh022199@ecust.edu.cn