阳大海
生物工程专业：博士生导师/硕士生导师
生物学专业：博士生导师/硕士生导师
生物与医药专业（生物工程领域）：工程博士生导师/工程硕士生导师
【个人简介】
阳大海，博士，华东理工大学生物工程学院教授，博士生导师。国家自然科学基金优秀青年基金获得者、国家重点研发计划（青年科学家）项目负责人，入选中国科协“青年人才托举工程”、上海市“曙光学者”、“浦江人才”和“晨光计划”等项目。现担任全国高校“双带头人”教师党支部书记“强国行”专项行动团队负责人、农业农村部水生动物疫病专业实验室副主任、中国水产学会水产动物免疫学专业委员会委员、《Zoological Research》、《水产科技情报》杂志编委和《渔业科学进展》杂志青年编委。迄今，已在Immunity、Nature Communications、Cell Reports、Fundamental Research、Reviews in Aquaculture和Journal of Immunology等学术期刊发表论文40余篇，先后荣获上海市自然科学二等奖(2/5)、上海海洋科学技术一等奖等。
【研究方向】
课题组聚焦先天免疫记忆(训练免疫) 这种新的免疫应答模式，以小鼠、斑马鱼、大菱鲆等物种为研究对象，系统鉴定并拓展了对训练免疫效应细胞功能的认识，阐明了训练免疫保护肝损伤和缓解感染性肠炎的分子机制，形成了基于训练免疫理论的免疫增强剂、佐剂、新型疫苗设计与效果评价方案，为免疫生物制品的研发与评价提供了新思路。
围绕上述基础研究领域，课题组将持续深入地开展以下工作：
（1）采用多组学（单细胞转录组、代谢组、表观遗传组等）、反向遗传操作（基因编辑）等技术，鉴定介导训练免疫激活的关键细胞并解析其介导的免疫记忆机制，探索其在调控感染性器官损伤、肠炎等疾病发生发展过程中发挥的功能；
（2）以解析训练免疫激活关键分子靶标为导向，开展抗感染药物的筛选、免疫生物制品（新型疫苗、佐剂、免疫增强剂等）的设计和活性评价。
【科研项目】
1. 上海市教委/上海市教育发展基金会“曙光计划”，24SG31，主持，在研；
2. 国家重点研发计划青年科学家项目（海洋农业与淡水渔业科技创新专项），2022YFD2402000，主持，在研；
3. 国家重点研发计划青年科学家项目（深海和极地关键技术与装备专项)，2022YFC2804300，参加，在研；
4.国家自然科学基金优秀青年科学基金，32122090，主持，结题；
5.上海市“科技创新行动计划”面上项目，20ZR1415500，主持，结题；
6.中国科协第二批“青年人才托举工程”，2016QNRC001，主持，结题；
7.国家自然科学基金青年项目，31602187，主持，结题；
8.上海市教委“晨光计划”，16CG33，主持，结题；
9.上海市人事局“浦江人才”，2016PJD020，主持，结题。
【教学工作】
1. 本科生专业选修课《免疫学》（秋季学期）；
2. 研究生专业选修课《现代疫苗学技术与进展》（秋季学期）。
【科研成果】
(一) 代表性论文（按时间倒序）
[1] Zhuang Wang, Yuanyuan Liu, Jing Hu, Xinwei You, Jin Yang, Yuanxing Zhang, Qin Liu, Dahai Yang*. Tissue-resident trained immunity in hepatocytes protects against septic liver injury in zebrafish, Cell Reports 2024,43(6): 114324.
[2] Xinwei You#, Jin Yang#, Zhuang Wang, Qiyao Wang, Qin Liu, Yuanxing Zhang, Geert F. Wiegertjes*, Dahai Yang*. Progress and perspective of trained immunity in teleost fish, Reviews in Aquaculture 2024, 16(2): 732-740.
[3] Wei Wang, Ying Wang, Jin Yang, Qin Liu, Yuanxing Zhang, Dahai Yang*. NITR12+ NK cells release perforin to mediate IgMhi B cells killing in turbot (Scophthalmus maximus), Journal of Immunology 2023, 211(11): 1693-1700.
[4]Weijie Chen#, Jianchang Huang#, Wei Wang#, Ying Wang#,Hao Chen, Qiyao Wang, Yuanxing Zhang, Qin Liu*, Dahai Yang*, Multi-tissue scRNA-seq reveals immune cell landscape of turbot (Scophthalmus maximus),Fundamental Research 2022, 2(4): 550-561.
[5]Di Mu#, Jin Yang#, Yu Jiang#, Zhuang Wang, Weijie Chen, Jianchang Huang, Yuanxing Zhang, Qin Liu, Dahai Yang*, Single-cell transcriptomic analysis reveals neutrophil as orchestrator during β-glucan-induced trained immunity in a teleost fish, Journal of Immunology 2022, 209(4): 783-795.
[6]Jiatiao Jiang, Wenwen Wang, Fei Sun, Yuanxing Zhang, Qin Liu, Dahai Yang*, Bacterial infection reinforces host metabolic flux from arginine to spermine for NLRP3 inflammasome evasion, Cell Reports 2021, 34(10): 108832.
[7] Weijie Chen, Jingjing Zhao, Di Mu, Zhuang Wang, Qin Liu, Yuanxing Zhang, Dahai Yang*, Pyroptosis mediates neutrophil extracellular trap formation during bacterial infection in zebrafish, Journal of Immunology 2021, 206(8): 1913-1922.
[8] Zhuang Wang, Zhaoyan Gu, Qing Hou, Weijie Chen, Di Mu, Yuanxing Zhang, Qin Liu, Zhihong Liu, Dahai Yang*, Zebrafish GSDMEb cleavage-gated pyroptosis drives septic acute kidney injury in vivo, Journal of Immunology 2020, 204(7): 1929-1942.
[9] Dahai Yang, Xin Zheng, Shouwen Chen, Zhuang Wang, Wenting Xu, Jinchao Tan, Mingyu Hou, Wenhui Wang, Zhaoyan Gu, Qiyao Wang, Ruilin Zhang, Yuanxing Zhang, Qin Liu*, Sensing of cytosolic LPS through caspy2 pyrin domain mediates noncanonical inflammasome activation in zebrafish, Nature Communications 2018, 9(1): 3052.
[10] Dahai Yang, Yuan He, Raul Muñoz-Planillo, Qin Liu*, Gabriel Núñez*, Caspase-11 requires the pannexin-1 channel and the purinergic P2X7 pore to mediate pyroptosis and endotoxic shock, Immunity 2015, 43(5): 923-932.
(二) 其他论文（按时间倒序）
[11] Jin Yang, Shu Cui, Boning Shao, Yanbo Zhao, Zhuang Wang, Qin Liu, Yuanxing Zhang, Dahai Yang*. ScRNA-seq reveals trained immunity-engaged Th17 cell activation against Edwardsiella piscicida-induced intestinal inflammation in teleost. Microbiological Research 2024, 289: 127912.
[12] Zhuang Wang, Jing Hu, Zhengkang Mei, Yuanxing Zhang, Qin Liu, Dahai Yang*, Mannan-oligosaccharide induces trained immunity activation and alleviates pathological liver injury in turbot (Scophthalmus maximus), Aquaculture 2024, 578: 740097.
[13] Zhuang Wang, Xinwei You, Yuanxing Zhang, Qin Liu, Dahai Yang*, Poly(I:C) induces anti-inflammatory response against secondary LPS challenge in zebrafish larvae, Fish Shellfish Immunol 2024, 144: 109285.
[14] Ying Wang, Wei Wang, Weijie Chen, Qin Liu, Yuanxing Zhang, Dahai Yang*, Characterization of ccl20a.3 and ccl20l as gene markers for Th17 cell in turbot,Fish Shellfish Immunol 2023, 141: 109005.
[15] Weijie Chen, Jing Hu, Jianchang Huang, Qin Liu, Qiyao Wang, Yuanxing Zhang, Dahai Yang*, Characterization of T-cell receptors and immunoglobulin heavy chains loci and identification of T/B cell clusters in teleost,Fish Shellfish Immunol 2023, 136: 108746.
[16] Jianchang Huang, Weijie Chen, Qiyao Wang, Yuanxing Zhang, Qin Liu, Dahai Yang*, Iso-Seq assembly and functional annotation of full-length transcriptome of turbot (Scophthalmus maximus) during bacterial infection,Mar Genom 2022, 63: 100954.
[17] Hao Chen, Xiaoming Wu, Zhaoyan Gu, Shouwen Chen, Xiangshan Zhou, Yuanxing Zhang, Qin Liu, Zhuang Wang*, Dahai Yang*, Zebrafish gasdermin E cleavage-engaged pyroptosis by inflammatory and apoptotic caspases, Dev Comp Immunol 2021, 124:104203.
[18] Wenhui Wang, Jinchao Tan, Zhuang Wang, Yuanxing Zhang, Qin Liu, Dahai Yang*, Characterization of the inflammasome component SmASC in turbot (Scophthalmus maximus), Fish Shellfish Immunol 2020, 100: 324-333.
[19] Yin Wen, Shouwen Chen, Zhiwei Jiang, Zhuang Wang, Jinchao Tan, Tianjian Hu, Qiyao Wang, Xiangshan Zhou, Yuanxing Zhang, Qin Liu, Dahai Yang*, Dysregulated haemolysin promotes bacterial outer membrane vesicles-induced pyroptotic-like cell death in zebrafish, Cell Microbiol 2019, e13010.
[20] Dahai Yang#, Xiaohong Liu#, Wenting Xu, Zhaoyan Gu, Cuiting Yang, Lingzhi Zhang, Jinchao Tan, Xin Zheng, Zhuang Wang, Shu Quan, Yuanxing Zhang, Qin Liu*, The Edwardsiella piscicida thioredoxin-like protein inhibits ASK1-MAPKs signaling cascades to promote pathogenesis during infection, PLoS Pathog 2019, 15(7): e1007917.
[21] Zhuang Wang, Lingyun Lin, Weijie Chen, Xin Zheng, Yuanxing Zhang, Qin Liu, Dahai Yang*, Neutrophil Plays critical role during Edwardsiella piscicida immersion infection in zebrafish larvae, Fish Shellfish Immunol 2019, 87: 565-572.
[22] Huifang Cao, Fajun Han, Jinchao Tan, Mingyu Hou, Yuanxing Zhang, DahaiYang*, Qin Liu*, Edwardsiella piscicida type III secretion system effector EseK inhibits mitogen-activated protein kinase phosphorylation and promotes bacterial colonization in zebrafish larva, Infect Immun 2018, 86: e00233-18.
[23] Wenting Xu, Zhaoyan Gu, Lingzhi Zhang, Yuanxing Zhang, Qin Liu, DahaiYang*, Edwardsiella piscicida virulence effector Trxlp promotes the NLRC4 inflammasome activation during infection, Microb Pathog 2018, 123: 406-504.
[24] Huifang Cao, Cuiting Yang, Shu Quan, Tianjian Hu, Lingzhi Zhang, Yuanxing Zhang, Dahai Yang*, Qin Liu*, Novel T3SS effector EseK in Edwardsiella piscicida is chaperoned by EscH and EscS to express virulence, Cell Microbiol 2017: e12790.
[25] Dahai Yang, Qin Liu*, Chunshan Ni, Shuai Li, Haizhen Wu, Qiyao Wang, Jingfan Xiao, Yuanxing Zhang, Gene expression profiling in live attenuated Edwardsiella tarda vaccine immunized and challenged zebrafish: insights into the basic mechanisms of protection seen in immunized fish. Dev Comp Immunol 2013, 40: 132-141.
[26] Dahai Yang, Qin Liu*, Mingjun Yang, Haizhen Wu, Qiyao Wang, Jingfan Xiao, Yuanxing Zhang*, RNA-seq liver transcriptome analysis reveals an activated mhc-i pathway and an inhibited mhc-ii pathway at the early stage of vaccine immunization in zebrafish,BMC Genomics 2012, 13: 319.
（三）合作发表论文（按时间倒序）
[27] Shouwen Chen, Shuxin Li, Hao Chen, Yuxin Gong, Dahai Yang, Yuanxing Zhang, Qin Liu*. Caspase-mediated LPS sensing and pyroptosis signaling inHydra. Science Advances 2023, 9: eadh4054.
[28] Shouwen Chen, Yuxin Gong, Shuxin Li, Dahai Yang, Yuanxing Zhang, Qin Liu*, Hydragasdermin-gated pyroptosis signalling regulates tissue regeneration. Dev Comp Immunol 2023, 149: 104904.
[29] Ying Wang, Yinhua Jin, Fei Sun, Yuanxing Zhang, Qin Liu, Qiyao Wang, Dahai Yang, Yibei Zhang*. The c-di-GMP signallingcomponent YfiR regulates multiple bacterial phenotypes and virulence inPseudomonas plecoglossicida. J Appl Microbiol 2023, 134: lxad157.
[30] Feizi Hu, Zhuang Wang*, Yuanxing Zhang, Dahai Yang, Qin Liu. TLR5b-mediated NF-κB activation promotes neutrophil recruitment to defenseEdwardsiella piscicidainfection in zebrafish. Aquaculture 2023,564: 739041.
[31] Jingjing Zhao, Weijie Chen, Yuanxing Zhang, Qin Liu, Dahai Yang, Zhuang Wang*, Bacterial infection induces pyroptotic signaling-mediated neutrophil extracellular traps (NETs) formation in turbot (Scophthalmus maximus), Fish Shellfish Immunol 2022, 127: 982-990.
[32] Lingzhi Zhang, JIatiao Jiang, Jin Zhang, Xiaohong Liu, Dahai Yang, Yuanxing Zhang, Qin Liu*. Edwardsiella piscicida interferes with classical endocytic trafficking and replicates in a specialized replication-permissive niche in nonphagocytic cells, J Bacteriol 2021, 203(16): e0050520.
[33] Hao Chen, Shuangfei Ding, Jinchao Tan, Dahai Yang, Yuanxing Zhang, Qin Liu*, Characterization of the Japanese Flounder NLRP3 inflammasome in restricting Edwardsiella piscicida colonization in vivo, Fish Shellfish Immunol 2020, 103: 169-180.
[34] Shouwen Chen, Xin Ma, Di Wu, Dahai Yang, Yuanxing Zhang, Qin Liu*, Scophthalmus maximus Interleukin-1β limits Edwardsiella piscicida colonization in vivo, Fish Shellfish Immunol 2019, 95: 277-286.
[35] Tianjian Hu, Ran Chen, Lingzhi Zhang, Zhuang Wang, Dahai Yang, Yuanxing Zhang, Xiaohong Liu, Qin Liu*, Balanced role of T3SS and T6SS in contribution to the full virulence of Edwardsiella piscicida, Fish Shellfish Immunol 2019, 93: 871-878.
[36] Jinchao Tan, Dahai Yang, Zhuang Wang, Xin Zheng, Yuanxing Zhang, Qin Liu*, EvpP inhibits neutrophils recruitment via Jnk-Caspy inflammasome signaling in vivo, Fish Shellfish Immunol 2019, 92: 851-860.
[37] Tianjian Hu, Lingzhi Zhang, Wei Wang, Dahai Yang, Jingfan Xiao, Yuanxing Zhang, Xiaohong Liu, Qin Liu*, Edwardsiella piscicida enters nonphagocytic cells via a macropinocytosis-involved hybrid mechanism, J Bacteriol 2019, 201: e00548-18.
[38] Mingyu Hou, Wenhui Wang, Feizi Hu, Yuanxing Zhang, Dahai Yang, Qin Liu*, Phosphothreonine lyase promotes p65 degradation in a mitogen-activated protein kinase/mitogen- and stress-activated protein kinase 1-dependent manner, Infect Immun 2019, 87: e00508-18.
[39] Lingzhi Zhang, Zhiwei Jiang, Shan Fang, Yajun Huang,Dahai Yang, Qiyao Wang, Yuanxing Zhang, Qin Liu*, Systematic identification of intracellular-translocated candidate effectors in Edwardsiella piscicida, Front Cell Infect Microbiol 2018, 8: 37.
[40] Hideki Hara, Sergey S Seregin,Dahai Yang, Koichi Fukase, Mathias Chamaillard, Emad S Alnemri, Naohiro Inohara, Grace Y Chen, Gabriel Núñez*, The NLRP6 inflammasome recognizes lipoteichoic acid and regulates gram-positive pathogen infection. Cell 2018, 175: 1651-1664.
[41] Hao Chen, Dahai Yang, Fajun Han, Jinchao Tan, Lingzhi Zhang, Jingfan Xiao, Yuanxing Zhang, Qin Liu*, The bacterial T6SS effector EvpP prevents NLRP3 inflammasome activation by inhibiting the Ca2+-dependent MAPK-Jnk pathway, Cell Host & Microbe 2017, 21: 47-58.
[42] Mingyu Hou, Ran Chen, Dahai Yang, Gabriel Núñez, Zhuang Wang, Qiyauo Wang, Yuanxing Zhang, Qin Liu*, Identification and functional characterization of EseH, a new effector of the type III secretion system of Edwardsiella piscicida, Cell Microbiol 2017, 19: e12638.
[43] Shan Fang, Lingzhi Zhang, Ying Lou, Dahai Yang, Qiyao Wang, Yuanxing Zhang, Qin Liu*, Intracellular translocation and localization of Edwardsiella tarda type III secretion system effector EseG in host cells, Microb Pathog 2016, 97: 166-171.
[44] Lingzhi Zhang, Chunshan Ni, Wenting Xu, Tongcheng Dai, Dahai Yang, Qiyao Wang, Yuanxing Zhuang, Qin Liu*, Intramacrophage infection reinforces the virulence of Edwardsiella tarda, J Bacteriol 2016, 198: 1534-1542.
[45] Yuan He, Melody Y. Zeng, Dahai Yang, Benny Motro, Gabriel Núñez*, NEK7 Is an essential mediator of NLRP3 activation downstream of potassium efflux, Nature 2016, 530: 354-357.
（四）申请专利情况
[46] 阳大海，王壮，何晶，刘琴，王蓬勃，王启要，张元兴，提高海水养殖鱼类抗病能力的组合制剂、其制备方法及应用，专利号：202210529727.3
[47] 阳大海，王壮，姜宇，刘琴，王蓬勃，王启要，张元兴，一种大菱鲆复合免疫增强剂及其制备方法和应用，专利号：202210283589.5
[48] 阳大海，王壮，杨秋茜，刘琴，王蓬勃，张元兴，检测大菱鲆血栓细胞的试剂及其应用，专利号：202410955405 .4
[49] 阳大海，王壮，犹昕，刘光磊，刘琴，张元兴，产黑素短梗霉来源的胞外β-葡聚糖在制备训练免疫诱导剂的应用, 专利号：202510222531.3
[50] 吴宵，马悦，王启要，刘晓红，姜博尹，刘琴，阳大海，张艺蓓，一种大菱鲆圆环病毒及其应用，专利号：202410581974.7
【联系方式】
联系地址：上海市徐汇区梅陇路130号阿华楼
联系邮箱：dahaiyang@ecust.edu.cn