马兴元
发布人:生工学院网站管理员  发布时间:2019-02-26   动态浏览次数:701

马兴元  

生物化学与分子生物学:博士生导师/硕士生导师

生物工程:博士生导师/硕士生导师

微生物学:硕士生导师

生物工程领域:硕士生导师


(一)个人简历:

马兴元博士,教授、博士生导师,生物反应器工程国家重点实验室 固定研究人员;生物药学实验室\课题组负责人。上海市“浦江学者”、国家“新药创制”重大专项负责人。1995年7月和1999年7月从山西农业大学和沈阳农业大学毕业,先后获得动物医学专业学士学位和硕士学位;2003年6月毕业于中国人民解放军军需大学(现吉林大学)获博士学位,师从殷震院士、朱平研究员;硕士与博士期间在军需大学研究所 (军事医学科学院 第11所) 全军基因工程重点实验室从事分子生物学与基因工程药物研究。2003-2005年华东理工大学生物工程博士后流动站工作;2010.12-2011.12在新加坡国立大学(MIT-NUS联合所)国家公派(A类)访问学者。


(二)主要成果:

持国家“新药创制”重大专项、国基金、教育部和上海市重点及人才基金项目12项、参与8项。在ACS Synthetic Biology, Scientific Reports、Bioresource Technology,Targeted Oncology、Anti-Cancer Drugs、Developmental and Comparative Immunology、Biomed Pharm、JB、MB、AMB等杂志上发表论文70余篇(其中IF>3共21篇),申请发明专利30项(授权7项),目前已经开发5个生物技术创新生物品种完成了实验室和临床前主要指标的评价,为后续推向临床研究奠定了重要基础。部分奖励:1.第13届国际生物技术大会(2008年)国际生物技术青年科学家奖。2.上海市生物工程学会 青年科学家学术年会(2008)刘新垣院士征文一等奖。3.中国生物工程学会学术年会(2015年)大会优秀论文一等奖。4.上海市28届优秀发明选拔赛三等奖(铜奖)、二等奖(银奖)。5.上海市2016年科技发明(生物医药)三等奖。


(三)研究领域、主要方向和相关学术兼职及部分学术奖励:

研究领域:分子细胞生物学与生物制品

研究方向:(1)蛋白多肽类生物医药制品(抗体\疫苗\酶);

(2)真核细胞基因组编辑与合成生物学;

(3)动物细胞3D培养与分化及干细胞制剂;

主要学术兼职:Drug Discoveries & Therapeutics编委;Immunology and Vaccine Study编委;Progress in Applied Microbiology编委;Current Pharmaceutical Biotechnology编委;《中华现代临床医学杂志》常务编委;《中国医药导报杂志》编委;《中国细胞生物学学报》、《中国生物工程杂志》特约Reviewer;国家卫生与健康委/国家科技部“新药创制”重大专项评审专家;国家科技部“动物传染病防控与健康养殖”重点研发计划 评审专家;国家自然科学基金委、教育部和上海市科委等多类项目和人才计划评审专家;中国生化与分子生物学会工业生化分会常务理事;中国药学会生化与生物技术药物专业委员会 委员;中国蛋白药物质量与技术联盟 理事、质量专家;中国生化制药工业协会 委员;中国生物工程学会会员。


(四)承担科研项目:

(1)国家自然科学基金项目:(主持:30873190、31670944)、(参与:81673345、30870253、31300660);

(2)国家科技部与卫计委“新药创制”重大专项:(主持:2009ZX09103-693、2015GKS-470[遴选入库跟踪项目])、(参与:2009ZX09306-008、2004AA2Z3801 [863计划]);

(3)教育部项目:交叉学科与重大项目培育基金重点项目(主持:WK0913002)、教育部博士点专项基金(主持:20070251011)、教育部新教师科研专项基金(参与:20120074120027);

(4)上海市项目: “浦江学者”人才计划(主持:13PJD012)、上海市自然科学基金(主持:05ZR14033)、上海市国家新药重大专项配套项目(主持:2009ZX09103-693)、上海市科委生物医药科技重点项目(主持:17431904600、054319928;参与:14431904300)、上海市博士后基金(10R21412000);

(5)其它项目:主持生物反应器国重室课题、国际开放合作项目(新加坡)、国内开放合作项目(厦门大学)、华东理工大学引进人才启动基金、军队课题、企业合作课题等,若干项。


(五)主要代表性论文:

1.Yanyao Li, XiaoluXie, Xingyuan Ma∗, HaipengLiu∗.Identification of a CqCaspase gene with antiviral activity from red claw crayfish Cheraxquadricarinatus. Developmental and Comparative Immunology. 2019 (91):101-107.

2.Chuan Zhang, An Li, Ruina Wang, Yan Cao, Han Jiang, SongyingOuyang,Xingyuan Ma∗, YimingLub∗. Recombinant expression, purification and bioactivity characterization of extracellular domain of human tumor necrosis factor receptor 1.Protein Expression and Purification. 2019 (155):21-26.

3.Wenpeng Wang, WenyunZheng, Dong Wu, Xiujuan He, Haipeng Liu*, Xingyuan Ma*. Precise editing the genome of CHO cell factories with dual-genes for high quality production of heterologous protein by CRISPR/Cas9 technology. ACS Synthetic Biology,2018, 7 (5):1259–1268.

4.Kefeng Liu, Dan Zheng, Hantian Lei, Jing Liu, Jiandu Lei,* Luying Wang,* andXingyuan Ma*. Development of Novel Lignin-Based Targeted Polymeric Nanoparticle Platform for Efficient Delivery of Anticancer Drugs. ACS Biomaterials Science & Engineering.2018, 4 (5): 1730–1737.

5.Fabiao Hu, Daxia Pan, WenyunZheng*, Xiujuan He, FuzhengRen, Yiming Lu*,Xingyuan Ma*. Elucidating respective functions of two domains BIR and C-helix of human IAP survivin for precise targeted regulating mitotic cycle, apoptosis and autophagy of cancer cells. Oncotarget. 2017; 8:113687-113700.

6.Yuling Chen, Wei Zhou, Min Li, Yanan Li, Lei Huang, Lei Jiang, Ruxin Zhu, Xingyuan Ma*, YingnanBian*,Mutation rates of 13 RM Y-STRs in a Han population from Shandong province, China. Forensic Science International: Genetics Supplement Series. 2017, 6(12) e346-e348.

7.Yan-yao Li, Xiao-xiao Chen, Feng-yu Lin, Qiu-fan Chen, Xing-yuan Ma*, Hai-peng Liua*. CqToll participates in antiviral response against white spot syndrome virus via induction of anti-lipopolysaccharide factor in red claw crayfish Cheraxquadricarinatus.Developmental and Comparative Immunology. 2017, 74(9): 217–226.

8.ZengjieZheng, Hailong Jiang, Yan Huang, Jie Wang, Lei Qiu, Zhenlin Hu, Xingyuan Ma*&Yiming Lu*. Screening of an anti-inflammatory peptide from Hydrophiscyanocinctus and analysis of its activities and mechanism in DSS-induced acute colitis. Scientific Reports, 6:25672, DOI: 10.1038/srep25672. 

9.HuaGuo, Na Zhang, Di Liu, Ping Wang*, Xingyuan Ma*.Cell permeable manganese superoxide dismutase (TAT-MnSOD) induced inhibitory effect on the proliferation of human heptoma. Biomed Pharmacother. 2016, 29(83):1379-1386.

10.Xingyuan Ma, Yi Zhang, Yanyan Kang, Linfeng Li, WenyunZheng*. A recombinant protein TmSm(T34A) can inhibit proliferation and proapoptosis to breast cancer stem cells(BCSCs) by down-regulating the expression of Cyclin D1.Biomed Pharmacother. 2016, 23(84):373-381.

11.Di Liu,Hua Guo, Wenyun Zheng, Na Zhang, Tianwen Wang, Ping Wang, Xingyuan Ma*.Discovery of the cell-penetrating function of A2 domain derived from LTA subunit of Escherichia coli heat-labile enterotoxin. Applied Microbiology and Biotechnology,2016:6(3):1-10.

12.Guoying Dai, WenyunZheng, Xingyuan Ma*, Ping Wang*.Multisite Mutation of Monomer Survivin with Enhanced Effect on Apoptosis Regulation of Breast Cancer Cells. Biomed Pharmacother.2015,69(2):111-118 

13.Nanjing Lin, WenyunZheng, Linfeng Li, Ping Wang, Xingyuan Ma*, A novel system enhancing the endosomal escapes of peptides promotes Bak BH3 peptide inducing apoptosis in lung cancer A549 cells.Targeted Oncology. 2014, 9(2): 163-170 

14.Tianwen Wang, Xingyuan Ma*, Hu Zhu, Aitao LI, Guocheng Du, Jian Chen. Available methods for assembling expression cassettes for synthetic biology. Applied Microbiology and Biotechnology. ApplMicrobiolBiotechnol. 2012 93(5): 1853-1863.

15.Li Hui, Zhang Qian, Wang Xiao li, Ma Xing yuan*, Lin Kuangfei*, Liu Yong di*,GuJi dong*. Biodegradation of benzene homologues in contaminated sediment of the East China Sea. Bioresource Technology, 2012, 124: 129-136.  

16.YuxinXu, WenyunZheng, Tianwen Wang, Ping Wang, Ling Zhu, Xingyuan Ma*. Genetic protein TmSm(T34A) enhances sensitivity of chemotherapy to breast cell lines as a synergistic drug to doxorubicin. Biomedicine & Pharmacotherapy, 2012, 66 (5): 368-372.

17.Hailong Chen,Xingyuan Ma*, Zhi Li, Qiaoyun Shi, WenyunZheng, Yang Liu, Ping Wang. Functionalization of single-walled carbon nanotubes enables efficient intracellular delivery of siRNA targeting MDM2 to inhibit breast cancer cells growth. Biomedicine & Pharmacotherapy, 2012, 66 (5):334-338.   

18.Xingyuan Ma, Jianhua Tan*, Dongzhi Wei, Pin Zhu, Manji Sun. High-level secretion and purification of recombinant acetylcholinesterase from human brain in P. pastoris and identification by chromogenic reaction. Applied Microbiology and Biotechnology. 2006, 72: 316-322.

19.Ma Xingyuan, ZhengWenyun, Wei Dongzhi, Ma Yushu, Wang Tianwen, Wang Jinzhia, Liu Qinghai, Yang Shengli, High-level expression, purification and pro-apoptosis activity of HIV-TAT-Survivin (T34A) mutant to cancer cells in vitro, Journal of Biotechnology, 2006, 123:367-378.

20.Na Zhang, Hua Guo, Wenyun Zheng, Tianwen Wang, Ping Wang, Xingyuan Ma*. Design and screening of a chimeric survivin-specific nanobody and its anticancer activities in vitro. Anti-Cancer Drugs. 2016,27(9):839-47.

21.Di Liu, Fabiao Hu, Wenpeng Wang, Dong Wu, Xiujuan He, WenyunZheng*, Haipeng Liu*, Xingyuan Ma* Elucidating bottlenecks of the efficient preparation of an AB5-hexamer mucosal adjuvant protein LTm by genetic engineering. Journal of Microbiology and Biotechnology. 2017, 27(8): 1461-147.

22.An Li, Chuan Zhang, Jie Wang, Junjie Wang, Hailong Jiang, Jianzhong Li, Xingyuan Ma*, Wen Zhang*, Yiming Lu*. Cloning, expression, purification and bioactivity evaluation of a thrombin-like enzyme from Deinagkistrodonacutus venom gland library. Biotechnology Letters, 2018, 40(1):93-102.  

23.Hui Liu, Xiaodan Cao, Ping Wang*, Xingyuan Ma*. pH-sensitivepHluorins as a molecular sensor for in situ monitoring of enzyme-catalyzed prodrug activation. Biotechnology and Applied Biochemistry, 2017, 64(4):482-489. 

24.Xingyuan Ma, Nanjing Lin, Yanyan Kang, Linfeng Li*, WenyunZheng*. Screening and identification of chicken BMSCs-specific McAbs from hybridoma cell lines used for discovering novel biomarkers of BMSCs. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 2016, 35(4):199-211.

25.Na Zhang, Hua Guo, Wenyun Zheng, Ping Wang, Xingyuan Ma*.The small and powerful nanobodies (Nbs) originating from peculiar animals used for therapy of cancers.International Journal of Cancer Studies & Research. 2016, 4: 1-7.

26.Di Liu, Na Zhang, WenyunZheng, HuaGuo, Xiaoli Wang, Tianwen Wang, Ping Wang, Xingyuan Ma*.A novel method of efficient preparation to mucosal adjuvant E. coliheat-labile enterotoxin mutant (LTm) by artificial self-assembly in vitro.Applied Biochemistry and Biotechnology.2016, 2(15):1-13

27.Wang T, Ma X*, Du G, Chen J. Overview of Regulatory Strategies and Molecular Elements in Metabolic Engineering of Bacteria.MolBiotechnol. 2012, 52(3): 300-308. 

28.Wang T, Li A, Ma X*, DU G, Chen J. A simple and universal method to express protein in unfused form. Protein PeptLett. 2012, 19(9): 930-933.

29.WenyunZheng, Yanyan Kang, Linfeng Li, YuxinXu, Xingyuan Ma*,Levels of effectiveness of gene therapies targeting survivin and its splice variants in human breast cancer cells. Drug Discoveries & Therapeutics. 2011; 5(6):293-298. 

30.Xingyuan Ma, Bi Yao, WenyunZheng, Linfeng Li*. Comparative study on characterization of recombinant B subunit of E. coli heat-labile enterotoxin (rLTB) prepared from E. coli and P. patoris. J. Microbiol. Biotechnol. 2010, 20(3):550-557.

31.Haiyi Zhang, Yu Zheng, QinghaiLiu, Xinyi Tao, WenyunZheng, Xingyuan Ma*, Dongzhi Wei*. Development of a fed-batch process for the production of anticancerdrug TATm-survivin(T34A) in Escherichia coli.Biochemical Engineering Journal. 2009, 43(2):163–168.

32.Li Zhao, Yu Zheng, Xingyuan Ma* and Dongzhi Wei*. Effects of over-expression of glycerol dehydrogenase and 1,3-propanediol oxidoreductase on bioconversion of glycerol into 1, 3-propandediol by Klebsiellapneumoniae under micro-aerobic conditions. Bioprocess and Biosystems Engineering, 2009, 32(3): 313-320.

33.Xingyuan Ma, WenyunZheng, Tianwen Wang, Dongzhi Wei, Yushu Ma. Optimization and high-level expression of a functional GST-tagged rHLT-B in E. coli and GM1 binding ability of purified rHLT-B. Journal of Microbiology, 2006, 44(6): 293-300. 

34.Xingyuan Ma, ZhengWenyun, Tianwen Wang. Leukocyte function-associated antigen-1 structure, function and application prospects. Protein and Peptide Letters, 2006, 13: 397-400.

35.Tianwen David Wang &Fei Ma &Xingyuan Ma& Ping Wang*. Spatially programmed assembling of oxidoreductases with single-stranded DNA for cofactor-required reactions. ApplMicrobiolBiotechnol.2015,99(8):3469-3477 

36.Liqun Wang, Yanyan Kang, WenyunZheng*, Linfeng Li, Lei Shi, Xingyuan Ma.Effect on apoptosis and cell cycle of recombinant double negative 4 dominant mutation Survivin (T34/117A) in breast cancer cell B-Cap-37. Biomedicine & Pharmacotherapy, 2014, 68(3): 277–284

37.RijunGui, Xueqin An∗, Hongjuan Su, WeiguoShen, Linyong Zhu, Xingyuan Ma, Zhiyun Chen, Xiaoyong Wang..Rhodamine 6G conjugated-quantum dots used for highly sensitive and selective ratiometric fluorescence sensor of glutathione. Talanta, 2012, 94 (5) 295-300. 

38.Dan Qiu, Xueqin An∗, Zhiyun Chen, Xingyuan Ma. Microstructure study of liposomes decorated by hydrophobic magnetic nanoparticles. Chemistry and Physics of Lipids. 2012,165 (5):563-570.

(六)授权发明专利:

1.专利名称: 一种肿瘤化疗药物的蛋白增敏剂,专利授权号:ZL201010608978.8 

2.专利名称:一种增强多肽药物从细胞内体逃离的蛋白载药系统及其应用;专利授权号:ZL201310178357.4

3.发明名称:一种内含肽介导的类弹性蛋白纯化重组蛋白的方法,专利授权号:ZL201110236772.1

4.专利名称:来自LT亚基的细胞穿膜肽LTA2作为细胞内药物运输载体的应用,专利授权号:ZL201410785006.4

5.专利名称:一种ssDNA-蛋白质酶法交联方法,专利授权号:ZL201310261812.7

6.发明名称:大肠杆菌热敏毒素B亚单位的真核表达方法,专利授权号:ZL 2005 1 0027957.6

7.发明名称:含有HIV转导结构域的生存素突变体及制备方法和应用,专利授权号:ZL 2005 1 0026847.8

8.发明名称:一种用于原核分泌、融合表达大肠杆菌热敏毒素B亚单位的载体,专利授权号:ZL 2005 1 0028084.0

9.发明名称:非甲醇诱导型毕赤酵母蛋白表达系统及其用途,专利公开号: CN101104859 

10.发明名称: 一种抗肿瘤蛋白质药物的质量稳定工艺,专利申请号: 201010616473.6; 专利公开号:CN102366628A 

11.发明名称: 单壁碳纳米管转载抗肿瘤药物技术及其应用,专利申请号: 20111003490.7专利公开号:CN102370988A

12.发明名称:纳米材料固载蛋白酶分子的方法及应用, 专利申请号:201110024154.0; 专利公开号:CN102373192A

13.发明名称: 一种survivin及其突变体转基因酵母系统及其应用,201110008959.6; 专利公开号: CN102367422A

14.发明名称:来自LT亚基的细胞穿膜肽LTA2作为细胞内药物运输载体的应用,专利申请号: 201410785006.4

15.发明名称:一种通过体外装配高效制备疫苗佐剂分子的新方法; 申请号:201410843982.0

16.发明名称:一种多抗原通用流感疫苗及制备方法,专利申请号: 201610101482.9

17.发明名称:抗肿瘤药物TATm-Survivin(T34A)的纯化复性方法专利公开号:CN101139388 

18.发明名称:生产二羟基丙酮的基因工程菌,其构建方法及其用途专利公开号:CN 101092604

19.发明名称:重组人甲状旁腺激素1-84的制备方法专利公开号:CN1861790 

20.发明名称:一种静息细胞转化甘油生产1,3-丙二醇的方法专利公开号:CN 101157939

21.发明名称:生产抗癌药物TAT(m)-Survivin(T34A)的工程菌的发酵放大工艺专利公开号: CN101157949 

22.发明名称:新一代Pro-A+G多糖亲和介质及抗体蛋白药物纯化技术,专利申请号:20151082347.1

23.发明名称:一种基于组合自剪切与蛋白支架的生物活性小肽制备方法,申请号:201611243905.7

24.发明名称:一种疫苗粘膜免疫佐剂分子的制备方法, 中国专利公开号:CN 102382853A.

25.发明名称:靶向survivin纳米抗体及其制备方法和应用,申请号:201611243867.5

26.发明名称:一种包载重组抗肿瘤蛋白TmSm的mPEG-PLGA纳米颗粒及其制备方法和应用,申请号:201611244413.X

27.发明名称:抑制WSSV感染的铁结合蛋白基因Cq‑Ferritin及其制备方法与应用;申请号: 201711076744

28.发明名称:基于CRISPR/Cas9基因编辑改造的CHO细胞株及其制备方法;申请号:201842818039.5

29.发明名称:抑制WSSV增殖的半胱天冬氨酸酶基因Cq-caspase及其蛋白抗病毒活性应用;申请号: 20181108987.7

30.发明名称:一种载盐酸阿霉素的TA-Fe(III)修饰的PLGA纳米颗粒的制备方法及其用途;申请号 : 20190106795.7

(七)教材/著作编写:

1.参编,《免疫学原理与技术》,高等教育出版社,出版时间:2016年9月

2.主编,《疫苗工程》(国家十一五规划重点教材),华东理工大学出版社,ISBN:9787562825845 出版时间: 2009.8

3.主译,《生物催化剂与酶工程》科学出版社,ISBN:9787030221957 出版时间:2008.9

4.主编,《国家法定猪病诊断与防制》,中国轻工业出版社,ISBN: 9787501958047出版时间:2007.6

5.副主编,《国家法定禽病诊断与防制》,中国轻工业出版社,ISBN:7501942722出版时间:2005.4

6.参编,《食品感官评价》,科学出版社,ISBN:9787030557193,出版日期:2018.01

7.副主编,《奶牛养殖顾问》辽宁科技出版社,出版时间:2004

8.编译审校,《生物技术和遗传工程词汇》(The FAO Biotechnology Glossary)(2010)联合国粮农组织(FAO)ISBN:1020-0541,出版时间:2010年5月

9.审校,面向21世纪课程教材—兽医传染病学》中国农业大学出版社,出版时间:2002

联系方式:

联系电话:021-64250135(O) E-mail:maxy@ecust.edu.cn

通讯地址:生物工程学院 国重室//生物药学研究室 

华东理工大学   实验十八楼1413-1417室

上海市徐汇区 梅陇路130号邮编200237