王华磊
发布人:生工学院网站管理员  发布时间:2018-01-17   动态浏览次数:146

王华磊


  生物化学与分子生物学专业:硕士生导师

  生物工程领域:工程硕士生导师



  王华磊,男,理学博士。2013年9月份华东理工大学生物化学与分子生物学专业毕业。主要研究方向为生物催化剂的高效开发、人工定制及其在手性药物中间体合成中的应用。2010年11月参与完成的“工业酶制剂的创制与应用关键技术”获上海市科技进步一等奖;2013年12月参与完成的“定向转化多元醇的细胞催化剂创制及其应用关键技术”获上海市技术发明一等奖。作为项目负责人主持国家自然科学基金面上项目、国家自然科学基金青年基金、中国博士后科学基金、华东理工大学基本科研业务费等4个纵向项目,并与永农生物科学有限公司、山东佰安瑞生物药业有限公司、枣庄市杰诺生物酶有限公司等合作开发横向项目3个。在Applied and Environmental Microbiology, Microbial Cell Factories, Applied Microbiology and Biotechnology等学术期刊发表SCI论文二十余篇,申请中国发明专利8项。


主要研究方向:

(1)以酶资源多样性、酶催化机理、理性设计方法学为研究内容的生物催化剂高效创制。

(2)以产品为导向、基于逆合成生物催化技术的手性药物中间体绿色高效合成工艺开发。


工作经历:

2016/03-至今,华东理工大学,生物工程学院,讲师

2013/12-2016/02,华东理工大学,生物工程学院,师资博士后

2009/2-2010/3,中国科学院,青岛生物能源与过程研究所,助理研究员

2007/7-2009/2,杭州恩然生物技术有限公司,助理研究员


参与或承担的项目:

1、国家自然科学基金面上项目,21776084,2018/01-2021/12,主持。

2、国家自然科学基金青年基金,21406068,2015/01-2017/12,主持。

3、中国博士后科学基金面上一等资助,2014M560308,2014/01-2015/12,主持。

4、华东理工大学基本科研业务费探索研究基金,222201514324,2016/01-2017/12,20万元,主持。

5、永农生物科学有限公司(横向课题),2017/01-2019/12,主持。

6、山东佰安瑞生物药业有限公司(横向课题),2014/09-2018/08,主持。

7、枣庄市杰诺生物酶有限公司(横向课题),2015/09-2020/12,主持。

8、国家自然科学基金面上项目,21676069,2017/01-2020/12,项目骨干。

9、国家自然科学基金青年基金,21606062,2017/01-2019/12,参与。


发表论文(*通讯作者):

1.Haiyang Fan, Lifeng Chen, Huihui Sun, Hualei Wang*, Qinghai Liu, Yuhong Ren, Dongzhi Wei. Characterization of a novel nitrilase, BGC4, from Paraburkholderiagraminis showing wide-spectrum substrate specificity, a potential versatile biocatalyst for the degradation of nitriles. Biotechnology Letters, 2017, 39(1): 1725–1731.

2.Haiyang Fan, Lifeng Chen, Huihui Sun, Hualei Wang*, Qinghai Liu, Yuhong Ren, Dongzhi Wei. Development of nitrilase-mediated process for phenylacetic acid production from phenylacetonitrile. Chemical Papers, 2017, 71(10): 1985–1992.

3.Haiyang Fan, Lifeng Chen, Huihui Sun, Hualei Wang*, Yuhong Ren, Dongzhi Wei. A novel nitrilase from Ralstoniaeutropha H16 and its application to nicotinic acid production. Bioprocess and Biosystems Engineering, 2017, 40(8):1271-1281.

4.Lifeng Chen, Haiyang Fan, Yiping Zhang, Wei Wei, Jinping Lin, DongzhiWei, Hualei Wang*. Enhancement of ethyl (S)-4-chloro-3-hydroxybutanoate production at high substrate concentration by in situ resin adsorption. Journal of biotechnology, 2017, 251:68-75.

5.Lifeng Chen, Yiping Zhang, Haiyang Fan, Kai Wu, Jinping Lin, Hualei Wang*, Dongzhi Wei. Efficient bioreductive production of (R)-N-Boc-3-hydroxypiperidine by a carbonyl reductase. Catalysis Communications, 2017, 97:5-9.

6.Lifeng Chen, Haiyang Fan, Yiping Zhang, Kai Wu, Hualei Wang*, Jinping Lin, Dongzhi Wei. Development of a practical biocatalytic process for (S)-N-Boc-3-hydroxypiperidine synthesis. Tetrahedron Letters, 2017, 58(16):1644–1650.

7.Yiping Zhang, Hualei Wang*, Lifeng Chen, Kai Wu, JingliXie, Dongzhi Wei. Efficient production of ethyl (R)-4-chloro-3-hydroxybutanoate by a novel alcohol dehydrogenase from Lactobacillus curieae S1L19.Journal of Molecular Catalysis B: Enzymatic. 2016, 134:51-60

8.WenyuanGao, Haiyang Fan, Lifeng Chen, Hualei Wang*, Dongzhi Wei. Efficient kinetic resolution of secondary alcohols using an organic solvent-tolerant esterase in non-aqueous medium. Biotechnology Letters. 2016,38(7):1165-1171

9.Kai Wu, Hualei Wang#*, Lifeng Chen, Haiyang Fan, Zhiqiang Zhao, Dongzhi Wei*. Practical two-step synthesis of enantiopure styrene oxide through an optimized chemoenzymatic approach.  Applied Microbiology and Biotechnology.2016, 100(20):8757-8767

10.Zhiqiang Zhao, Hualei Wang*, Yiping Zhang, Lifeng Chen, Kai Wu, Dongzhi Wei. Cloning and characterization of three ketoreductases from soil metagenome forpreparing optically active alcohols. Biotechnology Letters.2016, 38(10):1799-1808

11.Wenyuan Gao, Kai Wu, Lifeng Chen, Haiyang Fan, Zhiqiang Zhao, Bei Gao, Hualei Wang*, Dongzhi Wei*. A novel esterase from a marine mud metagenomic library for biocatalytic synthesis of short-chain flavor esters. Microbial Cell Factories, 2016, 15:41.

12.Kai Wu, Lifeng Chen, Haiyang Fan, Zhiqiang Zhao, Hualei Wang*, Dongzhi Wei*. Synthesis of enantiopure epoxide by ‘one pot’ chemoenzymatic approach using a highly enantioselective dehydrogenase. Tetrahedron Letters, 2016, 57(8):899-904.

13.Huihui Sun, WenyuanGao, Hualei Wang*, Dongzhi Wei. Expression, characterization of a novel nitrilase PpL19 from Pseudomonas psychrotoleranswith S-selectivity toward mandelonitrile present in active inclusion bodies. Biotechnology letters, 2016,38(3):455-461

14.Hualei Wang, Haiyang Fan, Huihui Sun, Li Zhao, Dongzhi Wei*. Process development for production of (R)-(-)-mandelic acid by recombinant Escherichia coli cells harboring nitrilase fromBurkholderiacenocepacia J2315. Organic Process Research & Development, 2015,19(12),2010-2016

15.Hualei Wang, Wenyuan Gao, Huihui Sun, Lifeng Chen, Lujia Zhang, Xuedong Wang*, Dongzhi Wei*. Protein engineering of nitrilase from Burkholderiacenocepacia J2315 for efficient and enantioselective production of (R)-o-chloromandelic acid. Applied and Environmental Microbiology, 2015, 81(24):8469-8477.

16.Huihui Sun, Hualei Wang*, WenyuanGao, Lifeng Chen, Kai Wu, Dongzhi Wei*. Directed evolution of nitrilase PpL19 from Pseudomonas psychrotolerans L19 and identification of enantiocomplementary mutants toward mandelonitrile. Biochemical and Biophysical Research Communications, 2015, 468(4):820-825.

17.Huihui Sun, WenyuanGao, Haiyang Fan, Hualei Wang*, Dongzhi Wei. Cloning, purification and evaluation of the enzymatic properties of a novel arylacetonitrilase from Luminiphilussyltensis NOR5-1B: a potential biocatalyst for the synthesis of mandelic acid and its derivatives. Biotechnology letters, 2015,37(8):1655–1661.

18.Kai Wu, Hualei Wang*,Huihui Sun,Dongzhi Wei*. Efficient kinetic resolution of phenyl glycidyl ether by a novel epoxide hydrolase from Tsukamurellapaurometabola. Applied Microbiology and Biotechnology, 2015, 99(22):9511-9521

19.Hualei Wang, Huihui Sun, WenyuanGao, Dongzhi Wei*. Efficient production of (R)-o-chloromandelic acid by recombinant Escherichia coli cells harboring nitrilase from Burkholderiacenocepacia J2315. Organic Process Research & Development, 2014, 18(6):767–773. 

20.Hualei Wang, Guinan Li, Mingyang Li, Dongzhi Wei*, Xuedong Wang*. A novel nitrilase from Rhodobactersphaeroides LHS-305-cloning, heterologous expression and biochemical characterization. World Journal of Microbiology and Biotechnology, 2014, 30(1):245-252.

21.Hualei Wang,Huihui Sun, Dongzhi Wei*. Discovery and characterization of a highly efficient enantioselective mandelonitrile hydrolase from BurkholderiacenocepaciaJ2315 by phylogeny-based enzymatic substrate specificity prediction.BMC Biotechnology, 2013, 13:14.

22.Kefeng Ni, Hualei Wang(并列第一作者), Li Zhao, Minjie Zhang, Siyuan Zhang, Yuhong Ren*, Dongzhi Wei*. Efficient production of (R)-(-)-mandelic acid in biphasic system by immobilized recombinant E.coli.Journal of Biotechnology, 2013, 167(4):433-440. 

23.Shuiqin Jiang, Lujia Zhang*, Zhiqiang Yao, BeiGao, Hualei Wang, Xiangzhao Mao, Dongzhi Wei*. Switching a nitrilase fromSyechocystis sp. PCC6803 to a nitrile hydratase by rationally regulating reaction pathways. Catalysis Science & Technology, 2017, 7, 1122-1128.

24.Jian Qiu, Erzheng Su*, Hualei Wang, WenwenCai, Wei Wang, Dongzhi Wei*. Cloning, overexpression, and characterization of a high enantioselective nitrilase from Sphingomonaswittichii RW1 for asymmetric synthesis of (R)-phenylglycine. Applied Biochemistry and Biotechnology, 2014, 173(2):365-377.

25.Lujia Zhang, Bo Yin, Chao Wang, Shuiqin Jiang, Hualei Wang, Dongzhi Wei*, Y Adam Yuan*. Structural insights into enzymatic activity and substrate specificity determination by a single amino acid in nitrilase from Syechocystis sp. PCC6803.  Journal of Structural Biology, 2014, 188(2):93-101.

26.KefengNi, XuZhou, LiZhao,HualeiWang,YuhongRen,DongzhiWei*. Magnetic Catechol-Chitosan with Bioinspired Adhesive Surface: Preparation and Immobilization of omega-Transaminase. Plos One, 2012, 7(7):e41101


专利:

1、魏东芝、许向阳、宋在伟、王华磊。一种生物法制备苯乙酸的方法。中国发明专利,申请号:201611110169.8。

2、魏东芝、许向阳、宋在伟、王华磊。一种生物法合成(R)‑邻氯扁桃酸的方法。中国发明专利,申请号:201611110170.0。

3、王华磊、谢有余、王继国、范海洋、魏东芝。一种S型的ω-转氨酶ATA-W12及其基因和应用,中国发明专利,申请号:201611055835.2。

4、王华磊、张一平、魏东芝。一种醇脱氢酶LC3及其基因和应用,中国发明专利,申请号:201610066375.7。

5、王华磊、范海洋、任宇红、魏东芝。一种生物法合成(R)-(-)-扁桃酸的方法,中国发明专利,申请号:201510334809.2。

6、王华磊、高文渊、魏东芝。酯酶est4、酯酶EST4、重组质粒和基因工程菌株及其应用,中国发明专利,申请号:201510342723.4。

7、王华磊、陈利锋、魏东芝。一种不对称转化制备(S)-4-氯-3-羟基丁酸乙酯的重组大肠杆菌及其应用,中国发明专利,申请号:201510098687.1。

8、王华磊、吴锴、魏东芝。一种环氧化物水解酶及其编码基因和应用,中国发明专利,申请号:201410475055.8。


联系方式:

办公室:实验十八楼707室

电话:021-64251803

E-mail address: hlwang@ecust.edu.cn