魏东芝

生物学专业：博士生导师/硕士生导师

生物工程专业：博士生导师/硕士生导师

生物与医药专业（生物工程领域）：博士生导师/硕士生导师

   国家二级教授，鲁华生物技术研究所所长，生物工程学院学术委员会主任，上海生物工程学会理事长。

   全国优秀科技工作者，国家重点学科-生物化工学科带头人，上海市重点学科-生物工程学科带头人，教育部跨世纪优秀人才、上海市科技领军人才，上海市优秀学科带头人, 中国科学院“百人计划”入选者。教育部生物技术与生物工程专业教指委副主任，中国微生物学会酶工程专业委员会副主任，中国生物工程学会常务理事。享受国务院特殊津贴和上海市特殊津贴，中国科协“九大”代表。

2002-2022年先后5次主持国家“科技重大专项”及重点研发项目，负责完成国家863、973课题、自然科学基金、国家科技攻关、上海市重点项目等20余项重大重点项目。发表SCI收录论文300余篇（包括Angewandte Chemie、Metabolic Engineering、Green Chemistry、Applied and Environmental Micorbiology、 Chemical Communications等国际顶级学术期刊），他引1万多次，发明专利70余项。获得国家新药临床证书3项，实现产业化项目30余项。已培养研究生500余人。

    获2015年国家技术发明二等奖，2010-2023年省部级技术发明或科技进步一等奖5项。

创建鲁华生物技术研究所及学术团队

研究领域：

研究领域：

1. 生物催化工程：酶/生物催化剂进化、定制，生物催化过程工程研究。

2. 合成生物学：面向医药、农药、化工、食品、化妆品及材料等领域，创建细胞工厂并推动国际领先的产业化应用。

代表性论文

2022

Lin Yang, Kaiyue Zhang, Meng Xu, Youyu Xie, Xiangqi Meng, Hualei Wang*, Dongzhi Wei*. Mechanism-Guided Computational Design of ω-Transaminase by Reprograming of High-Energy-Barrier Steps. Angew. Chem. Int. 2022,(52): 202212555.

Kun Liu, Yunsen Zhang, Ke Liu, Yunqiu Zhao, Bei Gao, Xinyi Tao, Ming Zhao, Feng-Qing Wang*, Dongzhi Wei*. De novo design of a transcription factor for a progesterone. Biosensors & Bioelectronics. 2022, 203: 113897.

Min Jiang, Yong-Jun Liu, Guo-Song Liu, Wei Zhou, Ya-Ling Shen, Bei Gao*,  Feng-Qing Wang*, Dong-Zhi Wei*.Sequential resolution of (S) and (R)-6-fluoro-chroman-2-carboxylic acid by two esterases in turn. Green Chemistry. 2022,24: 3235-3242.

Kun Liu, Feng-Qing Wang*, Ke Liu,  Yunqiu Zhao,  Bei Gao, Xinyi Tao, Dongzhi Wei*. Light-driven progesterone production by InP–(M. neoaurum) biohybrid system. Bioresources and Bioprocessing. 2022, 9: 9.

Xie D, Du L, Lin H, Su E, Shen Y, Xie J*,Dongzhi Wei*.In vitro-in silico screening strategy and mechanism of angiotensin I-converting enzyme inhibitory peptides from α-lactalbumin. Lebensmittel-Wissenschaft und-Technologie,2022,156: 112984.

Du L, Ro K-S, Zhang Y, Tang Y-J, Li Wan, Xie J*,  Dongzhi Wei*.Effects of Lactiplantibacillus plantarum X7021 on physicochemical properties, purines, isoflavones and volatile compounds of fermented soymilk. Process Biochemistry. 2022, 113: 150-157.

Xie D, Shen Y, Su E, Du L, Xie J*,  Dongzhi Wei*.The effects of angiotensin I-converting enzyme inhibitory peptide VGINYW and the hydrolysate of α-lactalbumin on blood pressure, oxidative stress and gut microbiota of spontaneously hypertensive rats. Food & Function. 2022, 13: 2743-2755

Zhao L, Zhu J, Xie J*, Dongzhi Wei*. Discovery of a novel acrylic acid formation pathway in Gluconobacter oxydans and its application in biosynthesis of acrylic acid from glycerol. Process Biochemistry. 2022, 118: 182-189

2021

Min Liu, Yang-Chen Lin, Jiao-Jiao Guo, Meng-Meng Du, Xinyi Tao, Bei Gao, Ming Zhao, Yushu Ma, Feng-Qing Wang*, Dongzhi Wei*. High-level production of sesquiterpene patchoulol in Saccharomyces cerevisiae. ACS Synthetic and Biology. 2021, 10: 158-172.

Xiangqi Meng, Lin Yang, Yan Liu, Huali Wang*, Yaling Shen, Dongzhi Wei*. dentification and Rational Engineering of a High Substrate-Tolerant Leucine Dehydrogenase Effective for the Synthesis of L-tert-Leucine. ChemCatChem. 2021,13(14):  3340-3349

Liuzhu Wang, Shiqing Diao, Yangyang Sun, Shuiqin Jiang, Yan Liu, Hualei Wang* , Dongzhi Wei*.Rational engineering of Acinetobacter tandoii glutamate dehydrogenase for asymmetric synthesis of L-homoalanine through biocatalytic cascades. Catalysis Science & Technology. 20211, 14: 208-4215

Youyu Xie, Feng Xu, Lin Yang, He Liu, Xiangyang Xu, Hualei Wang* Dongzhi Wei*.Engineering the large pocket of an (S)-selective transaminase for asymmetric synthesis of (S)-1-amino-1-phenylpropane. Catalysis Science & Technology. 2021, 11: 2461-2470.

 2020

Liu, Guo-Song, Li, Tian, Zhou, Wei, Jiang, Min,T ao, Xin-Yi, Liu, Min, Zhao, Ming, Ren, Yu-Hong, Gao, Bei*, Wang, Feng-Qing*, Wei, Dong-Zhi*. The yeast peroxisome: A dynamic storage depot and subcellular factory for squalene over production. Metabolic Engineering. 2020, 57：151-161

Yang, ZY, Fu, HW ,Ye, WJ ,Xie, YY , Liu, QH, Wang, HL, Dongzhi Wei*.Efficient asymmetric synthesis of chiral alcohols using high 2-propanol tolerance alcohol dehydrogenase SmADH2 via an environmentally friendly TBCR system. Catalysis Science & Technology. 2020, 10: 70-78.

2019

Jiajia Zhang, Yumeng Chen, Chuan Wu, Pei Liu, X Wei Wang*, Dongzhi Wei*. The transcription factor ACE3 controls cellulase activities and lactose metabolism via two additional regulators in the fungus Trichoderma reesei. Journal of Biological Chemistry. 2019, 294:18435-18450

2018

Zhongwei Yang, Hongling Wang, Yuxiao Wang, Yuhong Ren*, Dongzhi Wei*. Manufacturing Multienzymatic Reactors In Vivo by Self-Assembly to Improve the Biosynthesis of Itaconic Acid in Escherichia coli. ACS Synthetic Biology. 7(5), 1244-1250.

Zhiqiang Yao, Shuiqin Jiang, Lujia Zhang, * Bei Gao, Xiao He, John Z. H. Zhang, Dongzhi Wei *. Crius: A novel fragment-based algorithm of de novo substrate prediction for enzymes. PROTEIN SCIENCE. 2018, 27(8): 1526-1534.

Hao-Hao Liu, Li-Qin Xu, Kang Yao, Liang-Bin Xiong, Xin-Yi Tao, Min Liu, Feng-Qing Wang*, Dong-Zhi Wei*. Engineered 3-ketosteroid 9α-hydroxylases in Mycobacterium neoaurum: an efficient platform for production of steroid drugs. Applied and Environmental Microbiology. 2018, 84(14): E02777-17.

Min Liu, Liang-Bin Xiong, Xinyi Tao, Qing-Hai Liu, Feng-Qing Wang*, Dong-Zhi Wei*. Metabolic adaptation of Mycobacterium neoaurum ATCC 25795 in the catabolism of sterols for producing important steroid intermediates. Journal of Agricultural and Food Chemistry. 2018, 66（45）: 12141-12150.

Min Liu; Liang-Bin Xiong; Xinyi Tao; Qing-Hai Liu; Feng-Qing Wang*; Dong-Zhi Wei*. Integrated transcriptome and proteome studies reveal the underlying mechanisms for sterol catabolism and steroid production in Mycobacterium neoaurum. Journal of Agricultural and Food Chemistry. 2018, 66(34): 9147-9157.

Zhen-hai Li, Feng-Qing Wang*, Dong-Zhi Wei*. Self-cloning CRISPR/Cpf1 facilitated genome editing in Saccharomyces cerevisiae. Biorescources and Bioprocessing. 2018, 5(1):36.

Yumeng Chen, Yaling Shen, Wei Wang* and Dongzhi Wei*. Mn2+ modulates the expression of cellulase genes in Trichoderma reesei Rut.C30 via calcium signaling，Biotechnology for Biofuels. 2018，11(1):54.

Bei Gao, Shuiqin Jiang, Liuzhu Wang, Lujia Zhang, Dongzhi Wei*. Energy and conformation determine the enantioselectivity of enzyme Biochemical Engineering Journal. 2018, 129: 106-112

Lingmeng Li, Fengying Dong,  Lin Lin, Dannong He, Wei Wei*,  and Dongzhi Wei*. N-Terminal Domain Truncation and Domain Insertion-Based Engineering of a Novel Thermostable Type I Pullulanase from Geobacillus thermocatenulatus. Journal of Agricultural and Food Chemistry. 2018, 66(44): 10788-10798

Lingmeng Li, Fengying Dong, Lin Lin, Dannong He, Jingwen Chen, Wei Wei,* Dongzhi Wei*. Biochemical Characterization of a Novel Thermostable Type I Pullulanase Produced Recombinantly in Bacillus subtilis. Starch. 2018, 70, 1700179

Xie Jingli, Chen Xujun, Wu Junjie, Zhang Yanyan, Zhou Yan, Zhang Lujia, Tang Ya-Jie, Wei Dongzhi*. Antihypertensive effects, molecular docking study, and isothermal titration calorimetry assay of angiotensin I‑converting enzyme inhibitory peptides from Chlorella vulgaris. Journal of Agricultural and Food Chemistry. 2018, 66: 1359-1368

Jiawei Zhu, Jingli Xie, Liujing Wei, Jinping Lin, Li Zhao* , Dongzhi Wei*. Identification of the enzymes responsible for 3-hydroxypropionic acid formation and their use in improving 3-hydroxypropionic acid production in Gluconobacter oxydans DSM 2003. Bioresource Technology. 2018, 265,:328-333.

2017

Xinyi Tao, Ning Jia, Nenghui Cheng, Yuhong Ren, Xuni Cao, Min Liu, Dongzhi Wei*, Feng-Qing Wang*. Design and evaluation of a phospholipase D based drug delivery strategy of novel phosphatidyl-prodrug. Biomaterials. 2017, 131: 1-14.

Liang-Bin Xiong, Hao-Hao Liu, Li-Qin Xu, Wan-Ju Sun, Feng-Qing Wang*, Dong-Zhi Wei*. Improving the production of 22-hydroxy-23,24-bisnorchol-4-ene-3-one from sterols in Mycobacterium neoaurum by increasing cell permeability and modifying multiple. Microbial Cell Factories.

Ying Liu, Xin Gao, Dongzhi Wei*, Yuhong Ren*. Reversible Photocontrol of Lipase Activity by Incorporating a Photo switch into the Lid Domain .Chemphotochem. 2017, 89: 2799.

Zhongwei Yang, Xin Gao, Hui Xie,  Fengqing Wang,  Yuhong Ren* and Dongzhi Wei*. Enhanced Itaconic Acid Production by Self-Assembly of Two Biosynthetic Enzymes in Escherichia coli. Biotechnology and Bioengineering. 2017, 1 (9): 393-396.

Min Jiang, Lujia Zhang, Fengqing Wang, Jie Zhang, Guosong Liu, Bei Gao*, Dongzhi Wei*. Novel application of magnetic protein: Convenient one-step purification and immobilization of proteins. Scientific Reports. 2017, 7: 13329.

Shuiqin Jiang, Lujia Zhang,* Zhiqiang Yao, Bei Gao, Hualei Wang, Xiangzhao Mao, Dongzhi Wei*. Switching a nitrilase from Syechocystis sp PCC6803 to a nitrile hydratase by rationally regulating reaction pathways. Catalysis Science & Technology. 2017,  7 (5) : 1122-1128.

Guo Mingrong, Chen Xujun, Wu Junjie, Zhang Lujia, Huang Weixue, Yuan Ying, Fang Ming, Xie Jingli*, Wei Dongzhi*. Angiotensin I-converting enzyme inhibitory peptides from Sipuncula (Phascolosoma esculenta): Purification, identification, molecular docking and antihypertensive effects on spontaneously hypertensive rats. Process Biochemistry. 2017,63:84-95

Wei Wang⁎, Yumeng Chen, Dong-Zhi Wei⁎. Copper-mediated on-off control of gene expression in filamentous fungus Trichoderma reesei，Journal of Microbiological Methods. 2017,143:63-65.

近期代表性专利 

联系方式

上海市梅陇路130号311信箱 邮编:200237

电话: 021-64252078

手机（微信）: 13601699452

E-mail:  dzhwei@ecust.edu.cn