林金萍
发布人:生工学院网站管理员  发布时间:2016-10-19   动态浏览次数:508

  

  

林金萍

  生物化学与分子生物学专业:硕士生导师

   生物化工专业:硕士生导师

   生物工程领域:工程硕士生导师


2006年毕业于华东理工大学生物化工专业,获工学博士学位。现任华东理工大学生物工程学院、生物反应器工程国家重点实验室副研究员。2016年入选上海市青年科技英才。主持和参与国家自然科学基金、“973”课题、重大专项等项目14项。发表SCI论文40余篇。申请发明专利 12项,其中授权9项。获得国家技术发明二等奖1项、上海市技术发明一等奖1项和上海市科技进步奖一等奖2项。获得国家新药临床批件2项、生产批文2项和新药证书1项,实现成果转让1项。


主要研究方向:

1.酶的筛选、发酵、纯化和功能表征

2.酶的结构和功能解析与酶分子改造

3.生物催化与生物转化合成手性化合物、药物、精细化学品、食品添加剂及高值生物基产品


代表性论文*通讯作者)

1.Kangling Chen, Kefei Li, Jian Deng, Baoqi Zhang, Jinping Lin*, Dongzhi Wei. Carbonyl reductase identification and development of whole-cell biotransformation for highly efficient synthesis of (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol. Microbial Cell Factories. 2016.15(1):191

2.Huan Zhang, Lulu Shi, Xinlei Mao, Jinping Lin*, Dongzhi Wei. Enhancement of cell growth and glycolic acid production by overexpression of membrane-bound alcohol dehydrogenase in Gluconobacter oxydans DSM 2003. Journal of Biotechnology. 2016, 237: 18-24

3.Jian Deng, Kangling Chen, Zhiqiang Yao, Jinping Lin*, Dongzhi Wei*. Towards the computational design and engineering of enzymeenantioselectivity: A case study by a carbonyl reductase from Gluconobacter oxydans. Journal of biotechnology.2016,217:31-40

4.Kefei Li, Xinlei Mao, Liu Liu, Jinping Lin*, Ming Sun, Dongzhi Wei and Shengli Yang. Overexpression of membranebound gluconate2dehydrogenase to enhance the production of 2ketodgluconic acid by Gluconobacter oxydans. Microbial Cell Factories. 2016, 15(1):121-130

5.Yuanyuan Shi, Kefei Li, Jinping Lin*, Dongzhi Wei, Shengli Yang. Engineered expression vectors significantly enhanced the production of 2-keto-D-gluconic acid by Gluconobacter oxydans. Journal of Agricultural and Food Chemistry.2015, 63(22): 5492−5498

6.Xingxing Zhang, Baoqi Zhang, Jinping Lin*, Dongzhi Wei. Oxidation of ethylene glycol to glycolaldehyde using a highly selective alcohol dehydrogenase from Gluconobacter oxydans. Journal of Molecular Catalysis B: Enzymatic. 2015,112: 69–75

7.Rong Chen, Xu Liu, Jiale Wang, Jinping Lin*, Dongzhi Wei*. Cloning, expression, and characterization of an anti-Prelog stereospecific carbonyl reductase from Gluconobacter oxydans DSM2343. Enzyme and Microbial Technology. 2015, 70: 18–27

8.Jian Deng, Kangling Chen, Zhiqiang Yao, Jinping Lin, Dongzhi Wei*. Efficient synthesis of optically active halogenated aryl alcohols at highsubstrate load using a recombinant carbonyl reductase fromGluconobacter oxydans. Journal of Molecular Catalysis B: Enzymatic.2015, 118:1–7

9.Xu Liu, Rong Chen, Zhongwei Yang, Jiale Wang, Jinping Lin*, Dongzhi Wei. Characterization of a Putative Stereoselective Oxidoreductase from Gluconobacter oxydans and Its Application in Producing Ethyl (R)-4-Chloro-3-Hydroxybutanoate Ester. Molecular Biotechnology, 2014, 56:285–295

10.Daihuan Li, Jinping Lin*, Dongzhi Wei. Improving Gluconobacter oxydans performance in the in situ removal of the inhibitor for asymmetric resolution of racemic 1-phenyl-1,2-ethanediol. Bioresource Technology, 2014, 159: 327–333

11.Lulu Shi, Kefei Li, Huan Zhang, Xu Liu, Jinping Lin*, Dongzhi Wei*. Identification of a novel promoter gHp0169 for gene expression in Gluconobacter oxydans. Journal of Biotechnology. 2014, 175:69-74

12.Xu Liu, Chao Wang, Lujia Zhang, Zhiqiang Yao, Dongbing Cui, Liang Wu, Jinping Lin* , Y. Adam Yuan, Dongzhi Wei. Structural and mutational studies on an aldo-keto reductase AKR5C3 from Gluconobacter oxydans. Protein science. 2014 ,23(11):1540-9

13.Xu Liu, Zuanning Yuan, Y. Adam Yuan, Jinping Lin*, Dongzhi Wei. Biochemical and structural analysis of Gox2181, a new member of the SDR superfamily from Gluconobacter oxydans. Biochemical and Biophysical Research Communications. 2011,415: 410-415

14.Jian Wu, Minhua Li, Jinping Lin*, Dongzhi Wei. Highly Selective Oxidation of Benzyl Alcohol Using Engineered Gluconobacter Oxydans in Biphasic System. Current Microbiology. 2011, 62:1123-1127

15.Jian Wu, Jiale Wang, Minhua Li, Jinping Lin*, Dongzhi Wei*. Optimization of immobilization for selective oxidation of  benzyl alcohol by Gluconobacter oxydans using response surface methodology. Bioresource Technology. 2010, 101:8936-8941

16.Minghua Li, Jian Wu, Xu Liu, Jinping Lin*, Dongzhi Wei*, Hao Chen. Enchanced production of dihydroxyacetone from glycerol by overexpression of glycerol dehydrogenase in an alcohol dehydrogenase-deficient mutant of Gluconobacter oxydans. Bioresource Technology. 2010, 101:8294-8299


发明专利:

1.林金萍, 魏东芝,张行星。一种醇脱氢酶氧化二元伯醇的方法。ZL201310606518.5

2.林金萍, 魏东芝,张行星。一种应用微生物细胞催化法制备羟基醛的方法。 ZL201310601537.9

3.林金萍, 魏东芝,李克非。一种微生物细胞转化法生产2-酮基-D-葡萄糖酸的方法。ZL201310598049.7

4.林金萍, 魏东芝,施露露。一种氧化葡萄糖酸杆菌启动子及其应用。ZL201310597667.X

5.林金萍, 魏东芝,杨圆圆。一种硝基还原酶在芳香族硝基化合物降解还原中国的应用。ZL201310627211.3

6.林金萍,魏东芝,吴建。一种同时分析微生物发酵液中甘油和二羟基丙酮的方法。ZL200910052444.9

7.林金萍, 魏东芝, 李明华, 吴建。一种氧化葡萄糖酸杆菌(G.oxydans)的基因工程菌及其应用。ZL20091096972.1

8.魏东芝,林金萍,高琼。一种微生物不对称拆分制备(S)-苯乙二醇的方法。ZL200810038899.0

9.魏东芝,林金萍,张敏洁。一种酶法连续生产聚(ε-己内酯)的方法。ZL 200910045661.5

获奖:

1. 定向转化多元醇的生物催化剂创制及其应用关键技术。国家技术发明二等奖,2015

2. 定向转化多元醇的细胞催化剂创制及其应用关键技术。上海市技术发明奖,一等奖,2013

3. 手性非天然氨基酸生物催化制备技术及其应用。上海市科技进步奖,一等奖,2012

4. 工业酶制剂的开发与应用关键技术。上海市科技进步奖,一等奖, 2010

  

联系方式:

上海市梅陇路130311信箱 邮编:200237

办公室:鲁华生物技术研究所(实验14楼东楼)402室;

电话: 021-64251923; 传真: 021-64250068

E-mail: jplin@ecust.edu.cn