张元兴
发布人:生工学院网站管理员  发布时间:2016-10-19   动态浏览次数:1747

张元兴

生物工程专业:博士生导师/硕士生导师

生物化工专业:博士生导师/硕士生导师

生物工程领域:工程硕士生导师



  19821月毕业于华东化工学院化学工程系,获学士学位。19847月毕业于华东化工学院生物化工系,获硕士学位,19959月评为华东理工大学教授。1997年担任博士生导师。1998年享受国务院政府特殊津贴。

  曾任国家863高技术计划海洋技术领域专家组副组长,国务院学位委员会生物工程临时学科评议组专家,华东理工大学生物工程学院院长。受聘为生物反应器工程国家重点实验室学术委员会副主任,国家海洋食品工程技术研究中心专家指导委员会副主任,福建省海洋功能食品工程技术研究中心技术委员会主任,山东省水产动物免疫制剂工程研究中心专家委员会主任,福建省海洋生物资源开发利用协同创新中心学术委员会副主任,农业部水产养殖病害防治专家委员会专家,教育部水产种质资源发掘与利用重点实验室、农业部海洋渔业资源可持续发展重点实验室、农业部海洋与河口渔业资源及生态重点开放实验室、农业部东海海水健康养殖重点实验室、农业部东海与远洋渔业资源开发利用重点实验室、农业部渔业装备与工程技术重点实验室、广东省水产经济动物病原生物学及流行病学重点实验室学术委员会委员。担任Bioresouces and BioprocessingJournal of Biochemistry and Molecular Biology Research、中国生物制品学杂志、中国抗生素杂志、中国海洋药物、食品安全与质量检测学报编委。目前承担国家自然科学基金重点项目和面上项目。为本科生讲授上海市精品课程生物化学,为博士研究生开设生物反应器设计与放大课程,已指导67名硕士研究生和50名博士研究生毕业,8名博士后出站,正在指导硕士研究生10名,博士研究生8名。已在国内外发表论文430余篇,其中SCI收录228篇,出版著作2部,参编著作6部,获省部级科技进步奖6项,教学成果奖1项,中国石油和化学工业优秀科技图书一等奖1项,申请国家发明专利57项,其中获得授权48项;申请国际专利3项,其中获得授权美国专利2项。

学术梯队:

1.周祥山,博士,教授,博士生导师,中组部万人计划第一批科技创新领军人才,上海市科技启明星,山东省泰山学者。承担国家自然科学基金项目,主要从事微生物发酵和生物制药的研究。

2.刘琴,博士,教授,博士生导师,国家优秀青年基金获得者,上海市科技启明星,教育部新世纪人才,任阿华生物工程研究所所长,上海海洋动物疫苗工程技术研究中心主任。承担国家自然科学基金项目主要从事海洋生物技术研究。

3.王启要,博士,教授,博士生导师,上海市科技启明星和浦江学者,任生物工程学院副院长。国家鲆鲽类农业技术体系疾病防控岗位科学家。承担国家自然科学基金项目,参加国家公益性农业行业专项,主要从事海洋生物技术研究。

4.蔡孟浩,博士,副教授。承担上海市自然科学基金项目,主要从事海洋微生物的研究。

5.周勉,博士,副教授,上海市浦江学者和晨光学者。承担国家自然科学基金项目,主要从事微生物表达系统的研究。

6.肖婧凡,博士,副教授。承担国家自然科学基金项目,主要从事海洋生物技术的研究。

7.马悦,博士。承担国家自然科学基金项目,参加国家公益性海洋行业专项和国家鲆鲽类农业技术体系项目,主要从事海洋生物技术研究。

8.阳大海,博士,上海市浦江学者和晨光学者,入选中国科协青年人才托举计划。承担国家自然科学基金项目,参加自然科学基金重点项目,主要从事海洋生物技术研究。

9.刘晓红,博士。承担国家自然科学基金项目,主要从事海洋动物疫苗的研究。

10.范卫民,大学,高级工程师。主要从事生物反应器的研究和设计。

11.陆健,学士,高级工程师。主要从事生物反应器的研究和设计。

12.林雅惠,学士。实验研究和实验室管理工作。

  

主要的研究方面:

1. 海洋生物技术。研究海洋病原微生物的特性,掌握其致病规律,在分子水平上阐明细胞通讯相关的条件致病现象,研究病原细菌和宿主之间的互作机制,从基因水平上进行改造,开发减毒活疫苗和多价疫苗。目前承担着国家自然科学基金重点和面上项目、现代农业产业技术体系岗位科学家项目。

2. 微生物工程。研究甲醇酵母基因诱导水平的精密控制网络,构建新的蛋白表达体系。解析生物合成代谢途径和相关基因(簇),在毕赤酵母中重构代谢网络,高效异源生物合成小分子活性化合物。在海洋微生物培养中,阐明丝状菌次级代谢调控的规律,调控菌丝形态,高效合成活性化合物。目前承担着国家自然科学基金项目和企业委托项目。

3. 动物细胞培养。研究动物细胞的生理和代谢规律,开发无血清培养基,建立动物细胞培养生产蛋白药物的优化工艺。研究动物细胞表达外源基因的高效体系,构建适合于大规模无血清培养的重组细胞株。目前承担着企业委托项目。

  

著作:

1.王启要,刘琴,张元兴 (2008) 群体感应:细菌之间的语言. 叶勤主编:生物科学与工程. 北京:科学出版社. p43-67.

2.张元兴 (2008) 动物细胞培养工程. 叶勤主编:生物科学与工程. 北京:科学出版社. p160-171.

3.张元兴,易小萍,张立,孙祥明 (2007) 动物细胞培养工程. 北京:化工出版社.

4.张元兴,许学书 (2001) 生物反应器工程. 上海:华东理工大学出版社.

  

2017年发表和出版中论文:

1.Guanhua Yang, Gabriel Billings, Troy Hubbard, Joseph Park, Ka Yin Leung, Qin Liu, Brigid M. Davis, Yuanxing Zhang, Qiyao Wang*, Matthew K. Waldor. Time resolved transposon insertion sequencing reveals genome-wide fitness dynamics during infection. mBio, accepted.

2.Xiating Gao, Yang Liu, Huan Liu, Zhen Yang, Qin Liu, Yuanxing Zhang, Qiyao Wang*. Identification of the regulon of AphB and its essencial roles in LuxR and exotoxin Asp expression in the pathogen Vibrio alginolyticus. Journal of Bacteriology, accepted.

3.Yuan Wu, Yanna Ren, Menghao Cai*, Xiangshan Zhou, Yuanxing Zhang*. Transcription factor Agseb1 affects development, osmotic stress response and secondary metabolism in marine-derived Aspergillus glaucus. Journal of Basic Microbiology, accepted.

4.Yang Liu, Qing Zheng, Minjun Yang, Kaiyu Yin, Guanhua Yang, Xiaohui Zhou, Ka Yin Leung, Qin Liu, Yuanxing Zhang, Qiyao Wang*. Transcriptomic dissection of the horizontally acquired response regulator EsrB reveals its global regulatory roles in the physiological adaptation and activation of T3SS and the cognate effector repertoire in Edwardsiella piscicida during infection. Virulence, accepted.

5.Yue Ma, Qiyao Wang*, Wensheng Xu, Xiaohong Liu, Xiating Gao, Yuanxing Zhang*. Stationary phase-dependent accumulation of ectoine is an efficient adaptation strategy in Vibrio anguillarum against cold stress. Microbiological Research 2017, 205: 8~18.

6.Hui Li, Jingfan Xiao*, Ya Zhou, Qiyao Wang, Yuanxing Zhang. Sensitivity improvement of rapid Vibrio harveyi detection with an enhanced chemiluminescent-based dot blot. Letters in Applied Microbiology 2017, 65(3): 206~212.

7.Ying Yin, Qiang Fu, Wenhui Wu, Menghao Cai, Xiangshan Zhou, Yuanxing Zhang*. Producing novel fibrinolytic isoindolinone derivatives in marine fungus Stachybotrys longispora FG216 by rational amino precursors supply according to its biosynthesis pathway. Marine Drugs 2017, 15(7): 214.

8.Yang Liu, Yanan Gao, Xiaohong Liu, Qin Liu, Yuanxing Zhang, Qiyao Wang, Jingfan Xiao*. Transposon insertion sequencing reveals T4SS as the major genetic traits for conjugation transfer of multi-drug resistance pEIB202 from Edwardsiella. BMC Microbiology 2017, 17: 112.

9.Siwei Xiao, Yanyun Gao, Xiaolong Wang, Wei Shen, Jinjia Wang, Xiangshan Zhou, Menghao Cai*, Yuanxing Zhang. Peroxisomes targeted and tandem repeat multimer expressions of human antimicrobial peptide LL37 in Pichia pastoris. Preparative Biochemistry and Biotechnology 2017, 47(3): 229-235.

10.Shaomei Liu, Jiaxin Li, Yuan Wu, Yanna Ren, Qi Liu, Qiyao Wang, Xiangshan Zhou, Menghao Cai*, Yuanxing Zhang. De novo transcriptome sequencing of marine-derived Aspergillus glaucus and comparative analysis of metabolic and developmental variations in response to salt stress. Genes & Genomics 2017, 39(3): 317-329.

11.Xiaoxu Zhang, Yanyun Gao, Ying Yin*, Menghao Cai, Xiangshan Zhou, Yuanxing Zhang. Effective pH pretreatment and cell disruption method for real-time intracellular enzyme activity assay of a marine fungus covered with pigments. Preparative Biochemistry and Biotechnology 2017, 47(2): 211-217.

12.Yanyun Gao, Mengmeng Huang, Xiaoyue Sun, Xiaoxu Zhang, Yuanxing Zhang, Xiangshan Zhou*, Menghao Cai*. Sigle-site mutation of C363G or N463T strengthens thermostability improvement of IG181-182 deleted acidic α-amylase from deep-sea thermophile Geobacillus sp. Food Biotechnology 2017, 31(1): 57-71.

13.Mengmeng Huang, Yanyun Gao, Xiangshan Zhou, Yuanxing Zhang, Menghao Cai*. Regulating unfolded protein response activator HAC1p for production of thermostable raw-starch hydrolyzing α-amylase in Pichia pastoris. Bioprocess and Biosystems Engineering 2017, 40(3): 341-350.

14.Jinjia Wang, Xiaolong Wang, Lei Shi, Fei Qi, Ping Zhang, Yuanxing Zhang, Xiangshan Zhou*, Zhiwei Song*, Menghao Cai*. Methanol-independent protein expression by AOX1 promoter with trans-acting elements engineering and glucose-glycerol-shift induction in Pichia pastoris. Scientific Reports 2017, 7: 41850.

15.Zhongyang Sun, Jia Deng, Haizhen Wu*, Qiyao Wang, Yuanxing Zhang. Selection of stable reference genes for real-time quantitative PCR analysis in Edwardsiella tarda. Journal of Microbiology and Biotechnology 2017, 27(1): 112-121.

16.Kaiyu Yin, Qiyao Wang, Jingfan Xiao*, Yuanxing Zhang. Comparative proteomic analysis unravels a role for EsrB in the regulation of reactive oxygen species stress responses in Edwardsiella piscicida. FEMS Microbiology Letters 2017, 364(1): fnw269.

17.Hao Chen, Dahai Yang, Fajun Han, Jinchao Tan, Lingzhi Zhang, Jingfan Xiao, Yuanxing Zhang, Qin Liu*. The bacterial T6SS effector EvpP prevents NLRP3 inflammasome activation by inhibiting Ca2+-dependent MAPK-Jnk pathway. Cell Host & Microbe 2017, 21(1): 47-58.

18.Xiaohong Liu, HuaZhang, Chenglong Jiao, Qin Liu, Yuanxing Zhang, Jingfan Xiao*. Flagellin enhances the immunoprotection of formalin-inactivated Edwardsiella tarda vaccine in turbot. Vaccine 2017, 35(2): 369-374.

19.Yue Ma, Qiyao Wang, Xiating Gao, Yuanxing Zhang*. Biosynthesis and uptake of glycine betaine as cold-stress response to low temperature in fish pathogen Vibrio anguillarum. Journal of Microbiology 2017, 55(1): 44-55.

20.Mingyu Hou, Ran Chen, Dahai Yang, Gabriel Núñez, Zhuang Wang, Qiyao Wang, Yuanxing Zhang, Qin Liu*. Identification and functional characterization of EseH, a new effector of the type III secretion system of Edwardsiella piscicida. Cellular Microbiology 2017, 19(1): e12638.

21.Ying Xue, Chuixing Kong, Wei Shen, Chenxiao Bai, Yanna Ren, Xiangshan Zhou, Yuanxing Zhang, Menghao Cai*. Methylotrophic yeast Pichia pastoris as a chassis organism for polyketides synthesisvia the full citrinin biosynthetic pathway. Journal of Biotechnology 2017, 242: 64-72.

2016年以前发表的主要文章:

  

1.Ying Yin*, Menghao Cai, Xiangshan Zhou, Zhiyong Li, Yuanxing Zhang*. Polyketides in Aspergillus terreus: biosynthesis pathway discovery and application. Applied Microbiology and Biotechnology 2016, 100(18): 7787-7798.

2.Dan Gu, Min Guo, Minjun Yang, Yuanxing Zhang, Xiaohui Zhou*, Qiyao Wang*. A sE-mediated temperature gauge controls a switch from LuxR-mediated virulence gene expression to thermal stress adaptation in Vibrio alginolyticus. PLoS Pathogens 2016, 12(6): e1005645.

3.Lingzhi Zhang, Chunshan Ni, Wenting Xu, Tongcheng Dai, Dahai Yang, Qiyao Wang, Yuanxing Zhang, Qin Liu*. Intramacrophage infection reinforces the virulence of Edwardsiella tarda. Journal of Bacteriology 2016, 198(10): 1534-1542.

4.Xiaolong Wang, Qi Wang, Jinjia Wang, Peng Bai, Lei Shi, Wei Shen, Mian Zhou, Xiangshan Zhou, Yuanxing Zhang, Menghao Cai*. Mit1 transcription factor mediates methanol signaling and regulates alcohol oxidase 1 promoter in Pichia pastoris. Journal of Biological Chemistry 2016, 291(12): 6245-6261.

5.Tongcheng Dai, Na Li, Fajun Han, Hua Zhang, Yuanxing Zhang, Qin Liu*. AMP-guided tumour-specific nanoparticle delivery via adenosine A1 receptor. Biomaterials 2016, 83: 37-50.

6.Teng Chu, Yajun Huang, Mingyu Hou, Qiyao Wang, Jingfan Xiao, Qin Liu*, Yuanxing Zhang. In vivo programmed gene expression based on artificial quorum networks. Applied and Environmental Microbiology 2015, 81(15): 4984-4992.

7.John S. Y. Goh, Yingwei Liu, Haifeng Liu, Kah Fai Chan, Corrine Wan, Gavin Teo, Xiangshan Zhou, Fusheng Xie, Peiqing Zhang, Yuanxing Zhang*, Zhiwei Song* (2014) Highly sialylated recombinant human erythropoietin production in large-scale perfusion bioreactor utilizing CHO-gmt4 (JW152) with restored GnT I function. Biotechnology Journal 9(1): 100-109.

8.Pham Ngoc Tu, Yamin Wang, Menghao Cai, Xiangshan Zhou*, Yuanxing Zhang* (2014) Co-expressions of protein tyrosine kinases EGFR-2 and PDGFRβ with protein tyrosine phosphatase 1B in Pichia pastoris. Journal of Microbiology and Biotechnology 24(2): 152-159.

9.Yingwei Liu, Xiangshan Zhou, Zhiwei Song*, Yuanxing Zhang* (2014) Sodium butyrate enhances the acidic isoform content of recombinant human erythropoietin produced by Chinese hamster ovary cells. Biotechnology Letters 36(5): 907-911.

10.Haifeng Liu, Xiangshan Zhou*, Shousheng Tian, Xianghui Hao, Jinhua You, Yuanxing Zhang* (2014) Two-step transpeptidation of the insulin precursor expressed in Pichia pastoris to insulin ester via trypsin-catalyzed cleavage and coupling. Biotechnology and Applied Biochemistry 61(4): 408-417.

11.Lili Sheng, Yuanzhi Lv, Qin Liu, Qiyao Wang*, Yuanxing Zhang* (2013) Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Veterinary Microbiology162(2-4): 652-662.

12.Haifeng Liu, Xiangshan Zhou*, Fusheng Xie, Jinhua You, Yuanxing Zhang* (2013) An efficient trypsin digestion strategy for improving desB30 productivity from recombinant human insulin precursor fusion protein. Process Biochemistry 48(5-6): 965-971.

13.Huan Liu, Dan Gu, Xiaodan Cao, Qin Liu, Qiyao Wang*, Yuanxing Zhang* (2012) Characterization of a new quorum sensing regulator luxT and its roles in the extracellular protease production, motility, and virulence in fish pathogen Vibrio alginolyticus. Archives of Microbiology 194(6): 439-452.

14.Ming Liu, Shuang Wang, Tingguang Sun, Jiguo Su, Yuanxing  Zhang*, Junjie Yue*, Zhiwei Sun* (2012) Insight into the structure, dynamics and the unfolding property of amylosucrases: Implications of rational engineering on thermostability. PLoS One 7(7): e40441.

15.Dahai Yang, Qin Liu*, Mingjun Yang, Haizhen Wu, Qiyao Wang, Jingfan Xiao, Yuanxing Zhang* (2012) RNA-seq liver transcriptome analysis reveals an activated MHC-I pathway and an inhibited MHC-II pathway at the early stage of vaccine immunization in zebrafish. BMC Genomics13: 319.

16.Menghao Cai, Xiangshan Zhou*, Jian Lu, Weimin Fan, Jiushun Zhou, Chuanpeng Niu, Li Kang, Xunqian Sun, Yuanxing Zhang* (2012) An integrated control strategy for the fermentation of the marine-derived fungus Aspergillus glaucus for the production of anti-cancer polyketide. Marine Biotechnology 14(6): 665-671.

17.Menghao Cai, Xueqian Sun, Xiangshan Zhou*, Yuanxing Zhang* (2012) Roles of cobalt in biosynthesis stimulation of a cytotoxic compound from marine-derived Aspergillus glaucus. Process Biochemistry 47(12): 2267-2274.

18.Menghao Cai, Xiangshan Zhou*, Jian Lu, Weimin Fan, Chuanpeng Niu, Jiushun Zhou, Xueqian Sun, Li Kang, Yuanxing Zhang* (2011) Enhancing aspergiolide A production from a shear-sensitive and easy-foaming marine-derived filamentous fungus Aspergillus glaucus by oxygen carrier addition and impeller combination in a bioreactor. Bioresource Technology 102(3): 3584-3586.

19.Xiaodan Cao, Qiyao Wang, Qin Liu*, Haopeng Rui, Huan Liu, Yuanxing Zhang* (2011) Identification of a luxO-regulated extracellular protein Pep and its roles in motility in Vibrio alginolyticus. Microbial Pathogenesis 50(2):123-131.

20.Lu Cheng, Xiangming Sun, Xiaoping Yi, Yuanxing Zhang* (2011) Transient gene expression of a mouse homolog of Fce-Fcg fusion protein for anti-allergic function assay. Process Biochemistry 46(4): 858-863.

21.Xin Wang, Qiyao Wang*, Minjun Yang, Jingfan Xiao, Qin Liu, Haizhen Wu, Yuanxing  Zhang* (2011) QseBC controls flagellar motility, fimbrial hemagglutination and intracellular virulence in fish pathogen Edwardsiella tarda. Fish & Shellfish Immunology 30(3): 944-953.

22.Honghong He, Qiyao Wang, Lili Sheng, Qin Liu*, Yuanxing Zhang* (2011) Functional characterization of Vibrio alginolyticus twin-arginine translocation system: its roles in stress adaptation and virulence towards fish. Current Microbiology 62(4): 1193-1199.

23.Yanhua Pan, Wei Wei, Hongyan Xu, Qiyao Wang, Qin Liu, Haizhen Wu*, Yuanxing Zhang* (2011) Differential gene expression in Japanese flounder (Paralichthys olivaceus) induced by live attenuated Vibrio anguillarum. Aquaculture Research 42(7):1042-1049.

24.Huan Liu, Qiyao Wang*, Qin Liu, Xiaodan Cao, Cunbin Shi, Yuanxing Zhang* (2011) Roles of Hfq in the stress adaptation and virulence in fish pathogen Vibrio alginolyticus and its potential application as a target for live attenuated vaccine. Applied Microbiology and Biotechnology 91(2):353-364.

25.Lu Cheng, Xiangming Sun, Xiaoping Yi, Yuanxing Zhang* (2011) Large-scale plasmid preparation for transient gene expression. Biotechnology Letters 33(8): 1559-1564.

26.Yan Zhao, Qin Liu*, Xiuhua Wang, Lingyun Zhou, Qiyao Wang, Yuanxing  Zhang* (2011) Surface display of Aeromonas hydrophila GAPDH in attenuated Vibrio anguillarum to develop a novel multivalent vector vaccine. Marine Biotechnology 13(5):963-970.

27.Jingfan Xiao, Tao Chen, Qiyao Wang*, Qin Liu, Xin Wang, Yuanzhi Lv, Haizhen Wu, Yuanxing  Zhang* (2011) Search for live attenuated vaccine candidate against edwardsiellosis by mutating virulence-related genes of fish pathogen Edwardsiella tarda. Letters in Applied Microbiology 53(4): 430-437.

28.Xiaonuo Teng, Xiaoping Yi, Xiangming Sun, Yuanxing Zhang* (2011) Modeling and application of controlled-fed perfusion culture of CHO cells in a bioreactor. Chemical and Biochemical Engineering Quarterly 25(3): 385-394.

29.Wei Wei, Haizhen Wu*, Hongyan Xu, Teng Chu, Changwen Wu, Kangmei Chang, Yuanxing Zhang* (2010) Cloning and molecular characterization of lycC1INH genes in large yellow croaker (Pseudosciaena crocea). Fish & Shellfish Immunology 28(1): 221-227.

30.Xueqian Sun, Xiangshan Zhou*, Menghao Cai, Jiushun Zhou, Yuanxing Zhang* (2010) Significant stimulation of o-phthalic acid in biosynthesis of aspergiolide A by a marine fungus Aspergillus glaucus. Bioresource Technology 101(10):3609-3616.

31.Lingyun Zhou, Xiuhua Wang, Qin Liu*, Qiyao  Wang, Yan Zhao, Yuanxing Zhang* (2010) A novel multivalent vaccine based on secretary antigen-delivery induces protective immunity against Vibrio anguillarum and Aeromonas hydrophila. Journal of Biotechnology 146(1-2):25-30.

32.Haopeng Rui, Jennifer M. Ritchie, Roderick T. Bronson, John J. Mekalanos, Yuanxing  Zhang, Matthew K. Waldor* (2010) Reactogenicity of live-attenuated Vibrio cholerae vaccines is dependent on flagellins. Proceedings of the NationalAcademy of Sciences of the United States of America107(9):4359-4364.

33.Xiaodan Cao, Qiyao Wang, Qin Liu*, Huan Liu, Honghong He, Yuanxing Zhang* (2010) Vibrio alginolyticus MviN is a LuxO-regulated protein and affects cytotoxicity toward EPC cell. Journal of Microbiology and Biotechnology20(2): 271-280.

34.Xiao Zhang, Wei Wei, Haizhen Wu*, Hongyan  Xu, Kangmei Chang, Yuanxing Zhang* (2010) Gene cloning and characterization of ferritin H and M subunits from large yellow croaker (Pseudosciaena crocea). Fish & Shellfish Immunology 28(5-6):735-742.

35.Menghao Cai, Xiangshan Zhou*, Jiushun Zhou, Chuanpeng Niu, Xueqian Sun, Yuanxing  Zhang* (2010) Efficient strategy for enhancing aspergiolide A production by citrate feedings and its effects on sexual development and growth of marine-derived fungus Aspergillus glaucus. Bioresource Technology 101(15):6059-6068.

36.Cuihong Du, Xiaoping Yi, Yuanxing  Zhang* (2010) Expression and purification of soluble recombinant human endostatin in Escherichia coli. Biotechnology and Bioprocess Engineering 15(2):229-235.

37.Ping Zhang, Wenwen Zhang, Xiangshan Zhou*, Peng Bai, James M. Cregg, Yuanxing  Zhang* (2010) Catabolite repression of Aox in Pichia pastoris is dependent on hexose transporter PpHxt1 and pexophagy. Applied and Environmental Microbiology 76(18): 6108-6118.

38.Xin Wang, Qiyao Wang, Jingfan Xiao, Qin Liu*, Haizhen Wu, Yuanxing  Zhang* (2010) Hemolysin EthA in Edwardsiella tarda is essential for fish invasion in vivo and in vitro and regulated by two-component system EsrA-EsrB and nucleoid protein HhaEt. Fish and Shellfish Immunology 29(6):1082-1091.

39.Suxia Li, Haizhen Wu, Jian Zhao, Ling Ou, Yuanxing Zhang* (2010) A biochemistry and molecular biology experiment and evaluation system for biotechnology specialty students. Biochemistry and Molecular Biology Education 38(4): 271-275.

40.Lili Xu, Qiyao Wang, Jingfan Xiao, Qin Liu*, Xin Wang, Tao Chen, Yuanxing  Zhang* (2010)Characterization of Edwardsiella tarda waaL: roles in lipopolysaccharide biosynthesis, stress adaptation, and virulence toward fish. Archives of Microbiology 192(12):1039-1047.

41.Qiyao Wang, Minjun Yang, Jingfan Xiao, Haizhen Wu, Xin Wang, Yuanzhi Lv, Lili Xu, Huajun Zheng, Shengyue Wang, Guoping Zhao, Qin Liu*, Yuanxing Zhang* (2009) Genome sequence of the versatile fish pathogen Edwardsiella tarda provides insights into its adaptation to broad host ranges and intracellular niches. PLoS One 4(10): e7646.

42.Haopeng Rui, Qin Liu, Qiyao Wang, Yue Ma, Huan Liu, Cunbin Shi, Yuanxing Zhang* (2009) Role of alkaline serine protease Asp in Vibrio alginolyticus virulence and regulation of its expression by LuxO-LuxR regulatory system. Journal of Microbiology and Biotechnology 19(5): 431-438.

43.Wei Wei, Haizhen Wu, Hongyan Xu*, Tao Xu, Xiao Zhang, Kangmei Chang, Yuanxing Zhang* (2009) Cloning and molecular characterization of two complement Bf/C2 genes in large yellow croaker (Pseudosciaena crocea). Fish & Shellfish Immunology 27(2):285-295.

44.Xin Wang, Qiyao Wang*, Jingfan Xiao, Qin Liu, Haizhen Wu, Lili Xu, Yuanxing  Zhang* (2009) Edwardsiella tarda T6SS component evpP is regulated by esrB and iron, and plays essential roles in the invasion of fish. Fish and Shellfish Immunology 27(3): 469-477.

45.Yuanxing Zhang (2009) Approaches to optimizing animal cell culture process: substrate metabolism regulation and protein expression improvement. Advances in Biochemical Engineering/Biotechnology 113: 177-215.

46.Qiyao Wang, Zhao Yang, Qin Liu*, Yuanxing  Zhang* (2009) Surface-exposed expression of Edwardsiella tarda EseB in live attenuated Vibrio anguillarum based on novel surface display systems. Aquaculture Research 40(13):1459-1467.

47.Jingfan Xiao, Qiyao Wang, Qin Liu, Xin Wang, Huan Liu, Yuanxing Zhang* (2009) Isolation and identification of fish pathogen Edwardsiella tarda from mariculture in China. Aquaculture Research 40(1):13-17.

48.Menghao Cai, Xiangshan Zhou*,Xueqian Sun, Kejing Tao, Yuanxing Zhang* (2009) Statistical optimization of medium composition for aspergiolide A production by marine-derived fungus Aspergillus glaucus. Journal of Industrial Microbiology & Biotechnology 36(3):381-389.

49.Jingfan Xiao, Qiyao Wang*, Qin Liu, Lili Xu, Xin Wang, Haizhen Wu, Yuanxing  Zhang* (2009) Characterization of Edwardsiella tardarpoS: its roles in controlling serum resistance, chondroitinase activity, biofilm formation, and autoinducer synthetases expression. Applied Microbiology and Biotechnology 83(1):151-160.

50.Wei Wei, Hongyan Xu*, Qiyao Wang, Xiao Zhang, Kangmei Chang, Changwen Wu, Yuanxing  Zhang* (2009) Identification of differentially expressed genes in large yellow croaker (Pseudosciaena crocea) induced by attenuated live Vibrio anguillarum. Aquaculture 291(1-2):124-129.

51.Xueqian Sun, Xiangshan Zhou*, Menghao Cai, Kejing Tao, Yuanxing  Zhang* (2009) Identified biosynthetic pathway of aspergiolide A and a novel strategy to increase its production in a marine-derived fungus Aspergillus glaucus by feeding of biosynthetic precursors and inhibitors simultaneously. Bioresource Technology 100(18):4244-4251.

52.Jing Ye, Yue Ma, Qin Liu, Dongling Zhao, Qiyao Wang, Yuanxing Zhang* (2008) Regulation of Vibrio alginolyticus virulence by the LuxS quorum-sensing system. Journal of Fish Diseases 31(3):161-169.

53.Chun Wei, Xiangshan Zhou, Yuanxing Zhang* (2008) Improving intracellular production of recombinant protein in Pichia pastoris using an optimized preinduction glycerol-feeding scheme. Applied Microbiology and Biotechnology 78(2):257-264.

54.Zheyong Ma, Xiaoping Yi, Yuanxing  Zhang* (2008) Enhanced intracellular accumulation of recombinant HBsAg in CHO cells by dimethyl sulfoxide. Process Biochemistry 43(6):690-695.

55.Weiyuan Zhang, Xiaoping Yi, Xiangming Sun, Yuanxing Zhang* (2008) Surface modification of non-woven poly (ethylene terephthalate) fabric scaffold for improving cell attachment in animal cell culture. Journal of Chemical Technology and Biotechnology 83(6):904-911.

56.Yang Tian, Qiyao Wang, Qin Liu, Yue Ma, Xiaodan Cao, Lingyu Guan, Yuanxing Zhang* (2008) Involvement of LuxS in the regulation of motility and flagellar biogenesis in Vibrio alginolyticus. Bioscience, Biotechnology, and Biochemistry 72(4): 1063-1071.

57.Lingyun Zhou, Qin Liu*, Qiyao Wang, Yue Ma, Yuzhou Xu, Zhao Yang, Yan Zhao, Yuanxing Zhang* (2008) Secretary delivery of heterologous proteins in attenuated Vibrio anguillarum for potential use in vaccine design. Applied Microbiology and Biotechnology 79(6):1027-1034.

58.Zhao Yang, Qin Liu, Qiyao Wang, Yuanxing Zhang* (2008) Gene cloning and expression of major capsid protein of large yellow croaker iridovirus and its secretion based on a-haemolysin transport system. Journal of the World Aquaculture Society 39(6):774-780.

59.Zhao Yang, Qin Liu*, Qiyao Wang, Yuanxing Zhang* (2008) Novel bacterial surface display systems based on outer membrane anchoring elements from the marine bacterium Vibrio anguillarum. Applied and Environmental Microbiology 74(14):4359-4365.

60.Ting Xie, Qin Liu*, Fusheng Xie, Haifeng Liu, Yuanxing Zhang* (2008) Secretory expression of insulin precursor in Pichia pastoris and simple procedure for producing recombinant human insulin. Preparative Biochemistry and Biotechnology 38(3):308-317.

61.Haopeng Rui, Qin Liu, Yue Ma, Qiyao  Wang, Yuanxing Zhang* (2008) Roles of LuxR in extracellular alkaline serine protease A expression, extracellular polysaccharide production and mobility of Vibrio alginolyticus. FEMS Microbiology Letters 285(2):155-162.

62.Yuzhou Xu, Qin Liu*, Lingyun Zhou, Zhao Yang, Yuanxing  Zhang* (2008) Surface display of GFP by Pseudomonas syringae truncated ice nucleation protein in attenuated Vibrio anguillarum strain. Marine Biotechnology 10(6):701-708.

63.Yang Tian, Qiyao Wang, Qin Liu, Yue Ma, Xiaodan Cao, Yuanxing Zhang* (2008) Role of RpoS in stress survival, synthesis of extracellular autoinducer 2, and virulence in Vibrio alginolyticus. Archives of Microbiology 190(5): 585-594.

64.Qiyao Wang, Qin Liu, Xiaodan Cao, Minjun Yang, Yuanxing Zhang* (2008) Characterization of two TonB systems in marine fish pathogen Vibrio alginolyticus: their roles in iron utilization and virulence. Archives of Microbiology 190(5): 595-603.

65.Wenying Wang, Xiaoping Yi, Yuanxing Zhang* (2007) Gene transcription acceleration: main cause of hepatitis B surface antigen production improvement by dimethyl sulfoxide in the culture of Chinese hamster ovary cells. Biotechnology and Bioengineering 97(3):526-535.

66.Hongzhong Wu, Fan Yang, Shuya Cui, Yufeng Qin, Jianwen Liu*, Yuanxing Zhang* (2007) Hematopoietic effect of fractions from the enzyme-digested colla corii asini on mice with 5-fluorouracil induced anemia. The American Journal of Chinese Medicine 35(5): 853-866.

67.Lu Yang, Yue Ma, Yuanxing Zhang* (2007) Freeze-drying of live attenuated Vibrio anguillarum mutant for vaccine preparation. Biologicals 35(4):265-269.

68.Xiangming Sun, Yuanxing Zhang* (2007) A cell-detaching reactor for inoculation of anchorage-dependent CHO and Vero cells between stepwise-expanded bioreactors. Biotechnology Letters 29(5): 697-701.

69.Qin Liu, Pengbo Wang, Yue Ma, Yuanxing Zhang* (2007) Characterization of Vibrio alginolyticus fur gene and localization of essential amino acid sites in Fur by in vitro site-directed mutagenesis. Journal of Molecular Microbiology and Technology 13(1-3):15-21.

70.Liming Ouyang, Ling Ou, Yuanxing Zhang* (2007) An integrated teaching strategy for teaching biochemistry to biotechnology specialty students. Biochemistry and Molecular Biology Education 35 (4): 267-271.

71.Qiyao Wang, Qin Liu, Yue Ma, Haopeng Rui, Yuanxing Zhang* (2007) LuxO controls extracellular protease, hemolytic activities and siderophore production in fish pathogen Vibrio alginolyticus. Journal of Applied Microbiology 103(5):1525-1534.

72.Qiyao Wang, Qin Liu, Yue Ma, Yuanxing Zhang* (2007) Isolation, sequencing and characterization of cluster genes involved in the biosynthesis and transportation of the siderophore of marine fish pathogen Vibrio alginolyticus. Archives of Microbiology 188(4):433-439.

73.Haiyan Huang, Yihua Yu, Xiaoping Yi, Yuanxing Zhang* (2007) Nitrogen metabolism of asparagine and glutamate in Vero cells studied by 1H/15N NMR spectroscopy. Applied Microbiology and Biotechnology 77(2):427-436.

74.Fang Zhang, Xiangming Sun, Xiaoping Yi, Yuanxing Zhang* (2006) Metabolic characteristics of recombinant Chinese hamster ovary cells expressing glutamine synthetase in presence and absence of glutamine. Cytotechnology 51(1):21-28.

75.Anfeng Xiao, Xiangshan Zhou, Li Zhou, Yuanxing Zhang* (2006) Improvement of cell viability and hirudin production by ascorbic acid in Pichia pastoris fermentation. Applied Microbiology and Biotechnology 72(4): 837-844.

76.Haiyan Huang, Xiaoping Yi, Yuanxing Zhang* (2006) Improvement of Vero cell growth in glutamate-based culture by supplementing ammoniagenic compounds. Process Biochemistry 41(12):2386-2392.

77.Hechun Zhang, Jixun Zhan, Keman Su, Yuanxing Zhang* (2006) A kind of potential food additive produced by Streptomyces coelicolor: characteristics of blue pigment and identification of a novel compound, l-actinorhodin. Food Chemistry 95(2):186-192.

78.Junhui Li, Zhengyu Huang, Xiangming Sun, Pengyuan Yang, Yuanxing Zhang* (2006) Understanding the enhanced effect of dimethyl sulfoxideon hepatitis B surface antigenexpression in the culture of Chinese hamster ovary cells on the basis of proteome analysis. Enzyme and Microbiol Technology 38(3-4):372-380.

79.Junhui Li, Xiangming Sun, Yuanxing Zhang* (2006) Improvement of hepatitis B surface antigen expression by dimethyl sulfoxide in the culture of recombinant Chinese hamster ovary cells. Process Biochemistry 41(2):317-322.

80.Qin Liu, Yue Ma, Lingyun Zhou, Yuanxing Zhang* (2006) Gene cloning, expression and functional characterization of an acyl carrier protein AcpV from Vibrio anguillarum. Archives of Microbiology 185(2):159-163.

81.Haifeng Liu, Xiangshan Zhou, Yuanxing Zhang* (2006) A comparative investigation on different refolding strategies of recombinant human tissue-type plasminogen activator derivative. Biotechnology Letters 28(7):457-463.

82.Qin Liu, Yue Ma, Lingyun Zhou, Yuanxing Zhang* (2005) Gene cloning and functional characterization of a phosphopantetheinyl transferase from Vibrio anguillarum serotype O1. Achieves of Microbiology 183(1):37-44.

83.Subiao Lu, Xiangming Sun, Yuanxing Zhang* (2005) Insight into metabolism of CHO cells at low glucose concentration on the basis of the determination of intracellular metabolites. Process Biochemistry 40(5):1917-1921.

84.Juxing Chen, Xiangming Sun, Yuanxing Zhang* (2005) Growth and metabolism of marine fish Chinook salmon embryo cells: Response to lack of glucose and glutamine. Biotechnology Letters 27(6): 395-401.

85.Juxing Chen, Xiangming Sun, Li Zhang, Xiaoping Yi, Yuanxing Zhang* (2005) Mass cultivation of marine fish Chinook salmon embryo cells in bioreactor with low-serum medium. Aquaculture 249(1-4):35-45.

86.Mingfei Shao, Yue Ma, Qin Liu, Yuanxing  Zhang* (2005) Secretory expression of recombinant proteins in an attenuated Vibrio anguillarum strain for potential use in vaccines. Journal of Fish Diseases 28(6):723-728.

87.Xiangming Sun, Yuanxing Zhang* (2004) Glutamine cannot support recombinant CHO cell growth and maintenance in the absence of glucose. Process Biochemistry 39(6):719-722.

88.Li Zhang, Hong Shen, Yuanxing Zhang* (2004) Fed-batch culture of hybridoma cells in serum-free medium using an optimized feeding strategy. Journal of Chemical Technology and Biotechnology 79(2):171-181.

89.Xiaoping Yi, Xiangming Sun, Yuanxing Zhang* (2004) Effects of osmotic pressure on recombinant BHK cell growth and von willebrand factor (vWF) expression. Process Biochemistry 39(11):1817-1823.

90.Qin Liu, Yue Ma, Haizhen Wu, Mingfei Shao, Haifeng Liu, Yuanxing Zhang* (2004) Cloning, identification and expression of an entE homologue angE from Vibrio anguillarum serotype O1. Archives of Microbiology 181(4):287-293.

91.Weibin Zhou, Yuanxing Zhang* (2004) Purification and identification of recombinant hirudin and its degradation derivatives expressed in Pichia pastoris. Preparative Biochemistry & Biotechnology 34(3):239-252.

92.Jizhong Yang, Xiangshan Zhou, Yuanxing Zhang* (2004) Improvement of recombinant hirudin production by controlling NH4+ concentration in Pichia pastoris fermentation. Biotechnology Letter 26(12):1013-1017.

93.Yuanxing Zhang*, Lin Dai, Xiangming Sun (2003) Kinetic aspect of hepatitis B surface antigen production in recombinant Saccharomyces cerevisiae fermentation. Process Biochemistry 38(11):1593-1598.

94.Xiangming Sun, Yuanxing Zhang* (2003) Estimation of cell density in packed-bed bioreactor by lactate production rate. Biotechnology Letters 25(11):853-857.

95.Subiao Lu, Xiangming Sun, Chaoou Shi, Yuanxing Zhang* (2003) Determination of tricarboxylic acid cycle acids and other related substances in cultured mammalian cells by gradient ion-exchange chromatography with suppressed conductivity detection. Journal of Chromatography A 1012(2):161-168.

96.Xiangshan Zhou, Jian Lu, Weimin Fan, Yuanxing Zhang* (2002) Development of a responsive methanol sensor and its application in Pichia pastoris fermentation. Biotechnology Letters 24(8): 643-646.

97.Xiangshan Zhou, Yuanxing Zhang* (2002) Decrease of proteolytic degradation of recombinant hirudin produced by Pichia pastoris by controlling the specific growth rate. Biotechnology Letters 24(17):1449-1453.

98.Jingli Xie, Yuanxing Zhang*, Yonghong Wang, Yuanguang Li (2001) Mixotrophic cultivation of Platymonas subcordiformis. Journal of Applied Phycology 13(4):343-347.

99.Xiangming Sun, Yuanxing Zhang*, Wensong Tan, Yajing Zhou, Ping Hua (2000) Attachment kinetics of Vero cells onto CT3 microcarriers. Journal of Bioscience and Bioengineering 90(1):32-36.

  

联系方法:上海市梅陇路130号华东理工大学生物工程学院,200237

电话和传真:021-64253025

邮件:yxzhang@ecust.edu.cn