吴辉
发布人:生工学院网站管理员  发布时间:2016-10-19   动态浏览次数:6987

 


  

生物工程专业:博士生导师/硕士生导师

生物化工专业:硕士生导师

生物工程领域:工程硕士导师

   

 

吴辉,工学博士,副教授。现任中国轻工业生物基材料工程重点实验室副主任,中国化工学会生物化工专委会青年学者工作委员会委员,上海市生物工程学会合成生物学委员会委员,生物反应器工程国家重点实验室固定人员。教育部霍英东青年教师基金获得者2018)。2004年毕业于南京工业大学生物工程系,获工学学士学位。2009年毕业于华东理工大学发酵工程专业,获工学博士学位。同年进入中科院上海生命科学研究院植物生理生态研究所从事博士后研究工作。2011年来到誉有南方的哈佛之称的美国莱斯大学生物工程系从事博士后研究工作。20149月引进华东理工大学生物工程学院,入选华东理工大学“青年英才培育计划”(2018 A类、2015)等。2019年被破格遴选为博士生导师。作为负责人主持国家自然科学基金面上项目、青年项目、教育部“霍英东青年教师基金” 、上海市自然科学基金等项目;作为主要研究骨干参与科技部国家重点研发计划课题、863计划等。近年来以第一作者或通讯作者在Metabolic EngineeringBiotechnology AdvancesACS Synthetic BiologyBiotechnology and Bioengineering等本领域重点期刊上发表SCI收录论文近40篇。申请中国发明专利10项,已授权4项;申请实用新型专利1 项。 指导研究生获2018年度“华东理工大学-东阿阿胶杰出奖学金”,华东理工大学第十三届“华陆科技· 奋进杯”大学生课外学术科技作品竞赛二等奖;共同指导并带队本科生获2017年和2018年连续两次获得国际遗传工程机器大赛(iGEM)竞赛金奖,指导“大学生创新创业训练计划”上海市级项目。

 

研究方向:

1.微生物代谢工程生产平台化合物和生物活性物质

运用生物信息学及代谢工程的最新技术和方法,合理设计及重构微生物代谢途径,结合代谢调控策略,建立以可再生生物质资源(糖、甘油、乙酸、甲酸、CO2等)为原料的新型高效微生物细胞工厂,合成多种生物基化学品与生物基材料,如丁二酸,3-羟基丙酸,丙酮,异丙醇,生物聚酯,氨基酸,脂肪酸及其衍生物等。

2.合成生物学中代谢晶体管研究及其应用

代谢晶体管控制模式借鉴电子元器件晶体管的放大控制模式,通过调节细胞中微量关键物质的浓度,进而对整个细胞进行代谢重编程。

3. 基于微生物电化学合成的代谢工程与合成生物学

    运用代谢工程与合成生物学的策略,结合电化学平台技术与方法,搭建能利用一碳资源的自养型大肠杆菌细胞工厂平台,用于生产多种生物基化合物如丙酮,异丙醇,3-羟基丙酸,丁二酸等。

 

主持和参加的科研项目:

1.主持国家自然科学基金面上基金 2018.1-2021.12

2.主持霍英东教育基金会第十六届高等院校青年教师基金 2018.3-2021.3

3.主持上海市自然科学基金 2019.6-2022.6

4.主持国家自然科学基金青年科学基金 2015.1-2017.12

5.主持生工学院优秀青年教师“远志计划”2017.1-2018.12

6.主持工业生物催化教育部重点实验室开放课题 2015.12-2017.12

7.主持中央高校基本科研业务费探索研究基金  2015.7-2017.6

8.主持生工学院优秀青年教师培育计划,2015.07-2017.07

9.参与国家重点研发计划(2017YFB03093022017.07 - 2021.06

10.参与上海市“科技创新行动计划” 2013.9-2016.9

11.参与国家863计划“基因组规模系统代谢育种” 2012.11-2015.12

12.参与国家863计划“化学品生物转化过程与装备” 2012.11-2015.12

 

学术与社会兼职:

Frontiers in Microbiology, SCI收录IF=4.259副主编2019-

Frontiers in Bioengineering and Biotechnology, SCI收录IF=5.122编委2016-

Frontiers in Bioengineering and Biotechnology, SCI收录IF=5.122客座副主编2019

Bioresources and Bioprocessing, ESCI收录执行编辑2016-

Bioresources and Bioprocessing, ESCI收录客座主编2015-2016

美国微生物学会ASM会员2015-

美国工业微生物与生物技术学会SIMB会员2013-

中国化工学会生物化工专委会青年学者工作委员会委员(2017-2021

中国生物工程学会青年工作委员会委员(2019-2023

上海市生物工程学会合成生物学委员会委员(2018-2022

多个国际杂志如Biotechnology AdvancesMetabolic EngineeringBiotechnology and BioengineeringBiotechnology for BiofuelScientific ReportsMicrobial Cell FactoriesFrontiers in MicrobiologyJournal of Agricultural and Food ChemistryBioresource TechnologyBiotechnology JournalBiochemical Engineering JournalProcess BiochemistryBioprocess and Biosystems EngineeringBioresources and BioprocessingFrontiers in Bioengineering and BiotechnologyApplied Biochemistry and BiotechnologyBiotechnology and Applied Biochemistry等审稿人。多个国内杂志《生物工程学报》、《微生物学报》、《生物加工过程》等期刊审稿人。

 

本科和研究生教学:

1.本科专业必修课,微生物(全英文),48学时,3学分;

2.博士研究生专业课程,系统代谢工程(与花强教授合作),32学时,2学分;

3. iGEM竞赛导师,Bio-maker创新活动指导老师,大创指导老师。

 

代表性论文:  

2019:

1Qiaofei He, George N. Bennett, Ka-Yiu San, Hui Wu*. 2019. Biosynthesis of medium-chain ω-hydroxy fatty acids by AlkBGT of Pseudomonas putida GPo1 with native FadL in engineered Escherichia coli. Frontiers in Bioengineering and Biotechnology. doi: 10.3389/fbioe.2019.00273. (IF=5.122, JCR Q1)

2Hao Yang#, Bing Huang#, Zhimin Li, Qin Ye, Hui Wu*. 2019. Metabolic engineering of Escherichia coli carrying the hybrid acetone-biosynthesis pathway for efficient acetone biosynthesis from acetate. Microbial Cell Factories. 18:6. (IF=4.402, JCR Q1)

3. Jingxian Lu, Zhimin Li, Qin Ye, Hui Wu*. 2019. Effect of reducing the activity of respiratory chain on biosynthesis of poly(3-hydroxybutyrate-co-lactate) in Escherichia coli. Chinese Journal of Biotechnology. 35(1):59-69.

4Boliang Gao, Li Li, Hui Wu, Du Zhu, Min Jin, Wu Qu, Runying Zeng*. 2019. A novel strategy for efficient agaro-oligosaccharide production based on the enzymatic degradation of crude agarose in Flammeovirga pacifica WPAGA1. Frontiers in Microbiology. 10:1231. (IF=4.259, JCR Q1)

5Xiangwei Cui, Junxian Wan, Xing Zhang, Hui Wu, Zhimin Li*, Qin Ye. 2019. Efficient glutathione production in metabolically engineered Escherichia coli strains using constitutive promoters. Journal of Biotechnology. 289:39-45 (IF=2.533, JCR Q2)

6Bing Huang, Guocheng Fang, Hui Wu, Jibin Sun, Zhimin Li*, Qin Ye. 2019. Efficient biosynthesis of succinate from paper mill wastewater by engineered Escherichia coli. Applied Biochemistry and Biotechnology. doi: 10.1007/s12010-019-03066-2. (IF=2.14, JCR Q3)

2018:

7Jiapeng Tang, Zhenqing Qian, Hui Wu*. 2018. Enhancing cordycepin production in liquid static cultivation of Cordyceps militaris by adding vegetable oils as the secondary carbon source. Bioresource Technology. 268:60-67. (IF=6.669, JCR Q1)

8Bing Huang, Hao Yang, Guochen Fang, Xing Zhang, Hui Wu*, Zhimin Li*, Qin Ye. 2018. Central pathway engineering for enhanced succinate biosynthesis from acetate in Escherichia coli. Biotechnology and Bioengineering. 115:943-954. (*Co-corresponding) (IF=4.26, JCR Q1)

9Wei Li#, Hui Wu#, Mai Li, Ka-Yiu San*. 2018. Effect of NADPH availability on free fatty acid production in E. coli. Biotechnology and Bioengineering. 115:444-452. (#First two authors contributed to this paper equally) (IF=4.26, JCR Q1)

10Han Liu, Guochen Fang, Hui Wu*, Zhimin Li*, Qin Ye. 2018. L-cysteine production in Escherichia coli based on rational metabolic engineering and modular strategy. Biotechnology Journal. 13:e1700695. (*Co-corresponding) (IF=3.543, JCR Q2)

11. Feng Li#, Yuanxiu Li#, Liming Sun, Xiaoli Chen, Xingjuan An, Changji Yin, Yingxiu Cao, Hui Wu, Hao Song*. 2018. Modular engineering intracellular NADH regeneration boosts extracellular electron transfer of Shewanella oneidensis MR-1. ACS Synthetic Biology. 7(3):885-895. (IF=5.571, JCR Q1)

12Qing Li#, Bing Huang#, Qiaofei He, Jingxian Lu, Xun Li, Zhimin Li, Hui Wu*, Qin Ye. 2018. Production of succinate from simply purified crude glycerol by engineered Escherichia coli using two-stage fermentation. Bioresources and Bioprocessing. 5:41. (#First two authors contributed to this paper equally) (Feature Article)

13. Jiaqi Jiang, Bing Huang, Hui Wu*, Zhimin Li*, Qin Ye. Efficient 3-hydroxypropionic acid production from glycerol by metabolically engineered Klebsiella pneumoniae. Bioresources and Bioprocessing. 5:34. (*Co-corresponding)

2017:

14Qing Li, Bing Huang, Hui Wu*, Zhimin Li, Qin Ye. 2017. Efficient anaerobic production of succinate from glycerol in engineered Escherichia coli by using dual carbon sources and limiting oxygen supply in preceding aerobic culture. Bioresource Technology. 231:75-84. (IF=6.669, JCR Q1)

15Xing Zhang#, Hui Wu#, Bing Huang, Zhimin Li*, Qin Ye. 2017. One-pot synthesis of glutathione by a two-enzyme cascade using a thermophilic ATP regeneration system. Journal of Biotechnology. 241:163-169. (# First two authors contributed to this paper equally) (IF=3.163, JCR Q2)

2016:

16Qing Li#, Hui Wu#, Zhimin Li, Qin Ye*. 2016. Enhanced succinate production from glycerol by engineered Escherichia coli strains. Bioresource Technology. 218:217-223. (# First two authors contributed to this paper equally) (IF=6.669, JCR Q1)

17Yunjie Li, Bing Huang, Hui Wu*, Zhimin Li*, Qin Ye, Yi-Heng Percival Zhang. 2016. Production of succinate from acetate by metabolically engineered Escherichia coli. ACS Synthetic Biology. 5:1299-1307. (*Co-corresponding) (IF=5.571, JCR Q1)

18Jianhua Yang, Wei Li, Dezheng Wang, Hui Wu*, Zhimin Li*, Qin Ye. 2016. Characterization of bifunctional L-glutathione synthetases from Actinobacillus pleuropneumoniae and Actinobacillus succinogenes for efficient glutathione biosynthesis. Applied Microbiology and Biotechnology. 100(14):6279-6289. (*Co-corresponding) (IF=3.670, JCR Q2)

19Jing Zhang, Cong Quan, Cheng Wang, Hui Wu*, Zhimin Li*, Qin Ye. 2016. Systematic manipulation of glutathione metabolism in Escherichia coli for improved glutathione production. Microbial Cell Factories. 15:38. (*Co-corresponding) (IF=4.402, JCR Q1)

20Zhuan Cheng, Jiaqi Jiang, Hui Wu, Zhimin Li*, Qin Ye. 2016. Enhanced production of 3-hydroxypropionic acid from glucose via malonyl-CoA pathway by engineered Escherichia coli. Bioresource Technology. 200:897-904. (IF=6.669, JCR Q1)

21Dezheng Wang, Cheng Wang, Hui Wu*, Zhimin Li*, Qin Ye. 2016. Glutathione production by recombinant Escherichia coli expressing bifunctional glutathione synthetase. Journal of Industrial Microbiology and Biotechnology. 43:45-53. (*Co-corresponding) (IF=2.993, JCR Q2)

22Qin Ye*, Zhimin Li, Hui Wu. 2016. Principle and performance of gas self-inducing reactors and applications to biotechnology. Advances in Biochemical Engineering/Biotechnology. 152:1-33. (IF=2.341, JCR Q2)

2015:

23Cheng Wang, Jing Zhang, Hui Wu*, Zhimin Li*, Qin Ye. 2015. Heterologous gshF gene expression in various vector systems in Escherichia coli for enhanced glutathione production. Journal of Biotechnology. 214:63-68. (*Co-corresponding). (IF=3.163, JCR Q2)

24Hui Wu, Leepika Tuli, George N. Bennett, Ka-Yiu San*. 2015. Metabolic transistor strategy for controlling electron transfer chain in Escherichia coli. Metabolic Engineering. 28:159-168. (IF=7.808, JCR Q1)

25Hui Wu, George N. Bennett, Ka-Yiu San*. 2015. Metabolic control of respiratory levels in coenzyme Q biosynthesis-deficient Escherichia coli strains leading to fine-tune aerobic lactate fermentation. Biotechnology and Bioengineering. 112(8):1720-1726. (IF=4.26, JCR Q1)

26Hui Wu, Jane Lee, Mukund Karanjikar, Ka-Yiu San*. 2015. Simultaneous utilization of glucose and mannose from woody hydrolysate for free fatty acid production by metabolically engineered Escherichia coli. Bioresource Technology. 185:431-435. (IF=6.669, JCR Q1)

27Dan Wang, Hui Wu, Chandresh Thakker, Jared Beyersdorf, George N Bennett, Ka-Yiu San*. 2015. Efficient free fatty acid production in engineered Escherichia coli strains using soybean oligosaccharides as feedstock. Biotechnology Progress. 31:686-694. (IF=2.406, JCR Q2)

2014:

28Hui Wu, Mukund Karanjikar, Ka-Yiu San*. 2014. Metabolic engineering of Escherichia coli for efficient free fatty acid production from glycerol. Metabolic Engineering. 25:82-91. (IF=7.808, JCR Q1)

29Hui Wu, Ka-Yiu San*. 2014. Efficient odd numbered straight-chain free fatty acid production by metabolically engineered Escherichia coli. Biotechnology and Bioengineering. 111(11):2209-2219. (IF=4.26, JCR Q1)

30Yane Luo*, Tao Zhang, Hui Wu*. 2014. The transport and mediation mechanisms of the common sugars in Escherichia coli. Biotechnology Advances. 32:905-919. (IF=12.831, JCR Q1)

31Hui Wu, Jane Lee, Mukund Karanjikar, Ka-Yiu San*. 2014. Efficient free fatty acid production from woody biomass hydrolysate in metabolically engineered Escherichia coli. Bioresource Technology. 169:119-125. (IF=6.669, JCR Q1)

32Hui Wu, Ka-Yiu San*. 2014. Engineering Escherichia coli for odd straight medium chain free fatty acid production. Applied Microbiology and Biotechnology. 98(19):8145-8154. (IF=3.670, JCR Q2)

2006-2013:

33Hui Wu, Qing Li, Zhi-min Li*, Qin Ye*. 2012. Succinic acid production and CO2 fixation using a metabolically engineered Escherichia coli in a bioreactor equipped with a self-inducing agitator. Bioresource Technology. 107:376-384. (IF=6.669, JCR Q1)

34Yuan Liu, Hui Wu, Qin Li, Xuwei Tang, Zhi-min Li*, Qin Ye. 2011. Process development of succinic acid production by Escherichia coli NZN111 using acetate as an aerobic carbon source. Enzyme and Microbial Technology. 49(5):459-64. ( First two authors contributed to this paper equally) (IF=3.553, JCR Q2)

35Yang Gu, Yu Jiang, Hui Wu, Xudong Liu, Zhilin Li, Jian Li, Han Xiao, Zhaobing Shen, Hongjun Dong, Yunliu Yang, Yin Li, Weihong Jiang*, Sheng Yang*. 2011. Economical challenges to microbial producers of butanol: feedstock, butanol ratio and titer. Biotechnology Journal. 6(11):1348-1357. (IF=3.543, JCR Q2)

36Hui Wu, Zhi-min Li, Li Zhou, Jingli Xie, Qin Ye*. 2009. Enhanced anaerobic succinic acid production by Escherichia coli NZN111 aerobically grown on gluconeogenetic carbon sources. Enzyme and Microbial Technology. 44:165-169. (IF=3.553, JCR Q2)

37Hui Wu, Zhi-min Li, Li Zhou, Qin Ye*. 2007. Improved succinic acid production in the anaerobic culture of an Escherichia coli pflB ldhA double mutant as a result of enhanced anaplerotic activities in the preceding aerobic culture. Applied and Environmental Microbiology. 73:7837-7843. (IF=4.077, JCR Q1)

 

发明专利:

1Ka-Yiu San, George N. Bennett, Hui Wu. Metabolic transistors in bacteria. US Patent Number: 10,011,839 B2.

2Ka-Yiu San, Xixian Xie, Leepika Tuli, Hui Wu. Hydroxy- and dicarboxylic-fat synthesis by microbes. US patent Number: 9,487,804 B2.

3Ka-Yiu San, George N. Bennett, Hui Wu. Metabolic transistors in bacteria. US Patent Number: 9,441,253 B2.

4Ka-Yiu San, Xian Zhang, Hui Wu, Dan Wang. KAS III-free fatty acid synthesis. US Patent. Publication Number: 2018/0148746 A1.

5Ka-Yiu San and Hui Wu. Integrated biodiesel process. US patent. Publication Number: 2014/0335578 A1.

6Ka-Yiu San and Hui Wu. Microbial odd chain fatty acids. US patent. Publication Number: 2014/0193867 A1.

7. 吴辉,赖宁玉,赵舒欣,王文卓,黎冠忠. 利用乙酸生产3-羟基丙酸的代谢工程大肠杆菌菌株的构建方法及应用. 专利申请号:201910928013.8.

8吴辉,陆静娴,李志敏,叶勤. 一种提高大肠杆菌合成的聚(3-羟基丁酸-co-乳酸)中乳酸组分含量的方法. 专利申请号: 201810246568.X.

9. 陆健,谭高翼,王启要,吴辉,夏建业,李友元,李晔,李媛媛. 一种用于光合生物反应器的发光搅拌桨. 专利公开(公告)号:CN207646198U

10吴辉,杨昊,李志敏,黄兵,叶勤. 利用乙酸生产丙酮或异丙醇的代谢工程大肠杆菌菌株的构建方法与应用. 专利申请号: 201710978174.9.

11吴辉,黄兵,李志敏,杨昊,叶勤. 利用乙酸生产羟基丙酸的代谢工程大肠杆菌菌株的构建方法与应用. 专利申请号: 201710969403.0.

12李志敏, 黄兵, 吴辉, 房国辰, 叶勤. 一种利用造纸废液生物合成丁二酸的方法. 专利申请号: 201711142577.6.

13叶勤,李晴,李志敏吴辉. 利用重组大肠杆菌以甘油为碳源发酵生产琥珀酸的方法. 专利申请号: 201610305322.6.

14李志敏, 李运杰, 吴辉, 叶勤. 利用乙酸生产丁二酸的代谢工程大肠杆菌菌株构建方法和应用. 专利申请号: 201410599042.1.

15周通,刘映淼,孙兵兵,王祎,吴辉,杨俊杰等. L-色氨酸基因工程菌,其构建方法以及使用其发酵生产L-色氨酸的方法. ZL201010598350.

16叶勤李志敏吴辉. 利用四碳二元酸发酵固定二氧化碳的方法. ZL200810207564.

17吴辉李志敏叶勤. 大肠杆菌生产琥珀酸的方法. ZL200710135684.

 

联系方式:

上海市梅陇路130283信箱 邮编:200237

办公室:实验十八楼801

电话:021-64253701

E-mail: hwu@ecust.edu.cn