吴辉
发布人:生工学院网站管理员  发布时间:2016-10-19   动态浏览次数:333

 

  

生物工程专业:硕士生导师

生物化工专业:硕士生导师

生物工程领域:工程硕士导师

   

工学博士,副教授。2004年毕业于南京工业大学生物工程系,获工学学士学位。2009年毕业于华东理工大学发酵工程专业,获工学博士学位。同年进入中科院上海生命科学研究院植物生理生态研究所从事博士后研究工作。2011年来到誉有“南方的哈佛”之称的美国莱斯大学生物工程系从事博士后研究工作。20149月引进华东理工大学生物工程学院,成为生物反应器工程国家重点实验室固定人员,入选第二批“青年英才引进与培育计划”, 生工院第二批青年教师扶持计划、“远志计划”等。近年来以第一作者或通讯作者在Metabolic EngineeringBiotechnology AdvancesACS Synthetic BiologyApplied and Environmental MicrobiologyBiotechnology and Bioengineering等本领域重点期刊上发表SCI收录论文20多篇。申请美国专利5 项,已授权2项。申请中国发明专利5 项,已授权3项。申请PCT专利1项。担任Bioresources and Bioprocessing杂志Lead guest editor2015-2016)和Executive Editor2016-);多个国际杂志如Biotechnology and Bioengineering, Biotechnology for Biofuel, Microbial Cell Factories, Biotechnology Journal, Journal of Agricultural and Food Chemistry, Bioresource Technology, Biochemical Engineering JournalProcess BiochemistryBioprocess and Biosystems EngineeringBioresources and BioprocessingFrontiers in Bioengineering and BiotechnologyApplied Biochemistry and BiotechnologyBiotechnology and Applied Biochemistry等审稿人。


研究方向:

1.微生物代谢工程生产平台化合物和生物活性物质

运用生物信息学及代谢工程的最新技术和方法,对生物合成途径进行创建,设计和优化,建立以可再生生物质资源为原料的新型高效微生物细胞工厂,用于生产多种平台化合物和生物活性物质,如丁二酸,3-羟基丙酸,1,3 丙二醇,谷胱甘肽,短、中、长链脂肪酸及其衍生物等。


2.合成生物学中“代谢晶体管”研究及其应用

“代谢晶体管”控制模式借鉴电子元器件“晶体管”的放大控制模式,通过调节细胞中微量关键物质的浓度,进而控制细胞整个代谢流的分布。


教育经历:

2000-2004   南京工业大学,制药与生命科学学院,生物工程,工学学士

2004-2009   华东理工大学,生物工程学院,发酵工程,工学博士


研究工作经历:

2009.9-2011.4  中国科学院上海生命科学研究院植物生理生态研究所,博士后

2011.4-2014.7  美国莱斯大学乔治布朗工程学院生物工程系,博士后

2014.9-2015.9  华东理工大学生物工程学院,讲师,硕士生导师

2015.9-至今华东理工大学生物工程学院,副教授,硕士生导师

主持和参加的科研项目

1. 主持国家自然科学基金青年科学基金 2015.1-2017.12

2. 主持工业生物催化教育部重点实验室开放课题 2015.12-2017.12

3. 主持中央高校基本科研业务费探索研究基金  2015.7-2017.6

4. 生工学院优秀青年教师培育计划,2015.07-2017.07

5. 参与上海市科技创新行动计划 2013.9-2016.9

6. 参与国家863计划“基因组规模系统代谢育种” 2012.11-2015.12

7. 参与国家863计划“化学品生物转化过程与装备” 2012.11-2015.12


代表性论文:

2017:

1.Qing Li, Bing Huang, Hui Wu*, Zhimin Li, Qin Ye. 2017. Efficient anaerobic production of succinate from glycerol in engineered Escherichia coli by using dual carbon sources and limiting oxygen supply in preceding aerobic culture. Bioresource Technology. 231: 75-84.

2.Xing Zhang#, Hui Wu#, Bing Huang, Zhimin Li*, Qin Ye. 2017. One-pot synthesis of glutathione by a two-enzyme cascade using a thermophilic ATP regeneration system. Journal of Biotechnology. 241:163-169. (# First two authors contributed to this paper equally)

2016:

3.Qing Li#, Hui Wu#, Zhimin Li, Qin Ye*. 2016. Enhanced succinate production from glycerol by engineered Escherichia coli strains. Bioresource Technology. 218: 217-223. (# First two authors contributed to this paper equally)

4.Yunjie Li, Bing Huang, Hui Wu*, Zhimin Li*, Qin Ye, Yi-Heng Percival Zhang. 2016. Production of succinate from acetate by metabolically engineered Escherichia coli. ACS Synthetic Biology. 5:1299-1307. (*Co-corresponding)

5.Jianhua Yang, Wei Li, Dezheng Wang, Hui Wu*, Zhimin Li*, Qin Ye. 2016. Characterization of bifunctional L-glutathione synthetases from Actinobacillus pleuropneumoniae and Actinobacillus succinogenes for efficient glutathione biosynthesis. Applied Microbiology and Biotechnology. 100(14): 6279-6289. (*Co-corresponding)

6.Jing Zhang, Cong Quan, Cheng Wang, Hui Wu*, Zhimin Li*, Qin Ye. 2016. Systematic manipulation of glutathione metabolism in Escherichia coli for improved glutathione production. Microbial Cell Factories. 15:38. (*Co-corresponding)

7.Zhuan Cheng, Jiaqi Jiang, Hui Wu, Zhimin Li*, Qin Ye. 2016.Enhanced production of 3-hydroxypropionic acid from glucose via malonyl-CoA pathway by engineered Escherichia coli. Bioresource Technology. 200: 897-904.

8.Dezheng Wang, Cheng Wang, Hui Wu*, Zhimin Li*, Qin Ye. 2016. Glutathione production by recombinant Escherichia coli expressing bifunctional glutathione synthetase. Journal of Industrial Microbiology and Biotechnology. 43:45-53. (*Co-corresponding)

9.Qin Ye*, Zhimin Li, Hui Wu. 2016. Principle and performance of gas self-inducing reactors and applications to biotechnology. Advances in Biochemical Engineering/Biotechnology. 152:1-33.

2015:

10.Cheng Wang, Jing Zhang, Hui Wu*, Zhimin Li*, Qin Ye. 2015. Heterologous gshF gene expression in various vector systems in Escherichia coli for enhanced glutathione production. Journal of Biotechnology. 214:63-68. (*Co-corresponding).

11.Hui Wu,Leepika Tuli, George N. Bennett, Ka-Yiu San*. 2015. Metabolic transistor strategy for controlling electron transfer chain in Escherichia coli. Metabolic Engineering.28: 159-168.

12.Hui Wu, George N. Bennett, Ka-Yiu San*. 2015. Metabolic control of respiratory levels in coenzyme Q biosynthesis-deficient Escherichia coli strains leading to fine-tune aerobic lactate fermentation. Biotechnology and Bioengineering.112(8):1720-1726.

13.Hui Wu, Jane Lee, Mukund Karanjikar, Ka-Yiu San*. 2015. Simultaneous utilization of glucose and mannose from woody hydrolysate for free fatty acid production by metabolically engineered Escherichia coli. Bioresource Technology. 185: 431-435.

14.Dan Wang, Hui Wu, Chandresh Thakker, Jared Beyersdorf, George N Bennett, Ka-Yiu San*. 2015. Efficient free fatty acid production in engineered Escherichia coli strains using soybean oligosaccharides as feedstock. Biotechnology Progress. 31:686-694. 

2014:

15.Hui Wu, Mukund Karanjikar, Ka-Yiu San*. 2014. Metabolic engineering of Escherichia coli for efficient free fatty acid production from glycerol. Metabolic Engineering.25: 82-91.

16.Hui Wu,Ka-Yiu San*. 2014. Efficient odd numbered straight-chain free fatty acid production by metabolically engineered Escherichia coli. Biotechnology and Bioengineering. 111(11): 2209-2219.

17.Yane Luo*, Tao Zhang, Hui Wu*. 2014. The transport and mediation mechanisms of the common sugars in Escherichia coli. Biotechnology Advances. 32: 905-919.

18.Hui Wu, Jane Lee, Mukund Karanjikar, Ka-Yiu San*. 2014. Efficient free fatty acid production from wood hydrolysate in metabolically engineered Escherichia coli. Bioresource Technology. 169: 119-125.

19.Hui Wu,Ka-Yiu San*. 2014. Engineering Escherichia coli for odd straight medium chain free fatty acid production. Applied Microbiology and Biotechnology. 98(19): 8145-8154.

2006-2013:

20.Hui Wu, Qing Li, Zhi-min Li*, Qin Ye*. 2012. Succinic acid production and CO2 fixation using a metabolically engineered Escherichia coli in a bioreactor equipped with a self-inducing agitator. Bioresource Technology. 107:376-384.

21.Yuan Liu, Hui Wu, Qin Li, Xuwei Tang, Zhi-min Li*, Qin Ye. 2011. Process development of succinic acid production by Escherichia coli NZN111 using acetate as an aerobic carbon source. Enzyme and Microbial Technology. 49(5):459-64. ( First two authors contributed to this paper equally)

22.Yang Gu, Yu Jiang,Hui Wu, Xudong Liu, Zhilin Li, Jian Li, Han Xiao, Zhaobing Shen, Hongjun Dong, Yunliu Yang, Yin Li, Weihong Jiang*, Sheng Yang*. 2011. Economical challenges to microbial producers of butanol: feedstock, butanol ratio and titer. Biotechnology Journal. 6(11):1348-1357.

23.Hui Wu, Zhi-min Li, Li Zhou, Jingli Xie, Qin Ye*. 2009. Enhanced anaerobic succinic acid production by Escherichia coli NZN111 aerobically grown on gluconeogenetic carbon sources. Enzyme and Microbial Technology. 44:165-169

24.Hui Wu, Zhi-min Li, Li Zhou, Qin Ye*. 2007. Improved succinic acid production in anaerobic culture of a pflB ldhA double mutant of Escherichia coli by enhancing the anaplerotic activities in preceding aerobic culture. Applied and Environmental Microbiology. 73:7837-7843.


发明专利:

1.Ka-Yiu San, Xixian Xie, Leepika Tuli,Hui Wu. Hydroxy- and dicarboxylic-fat synthesis by microbes. US patent Number: 9,487,804.

2.Ka-Yiu San, George N. Bennett and Hui Wu. Metabolic transistors in bacteria. US Patent Number: 9,441,253.

3.Ka-Yiu San, Xian Zhang, Hui Wu, Dan Wang. KAS III-free fatty acid synthesis.Technical Description and Invention Disclosure are submitted for US patent application. 2015.

4.Ka-Yiu San and Hui Wu. Integrated biodiesel process. US patent. Application Number: 14/266,942.

5.Ka-Yiu San and Hui Wu. Microbial odd chain fatty acids. US patent. Application Number: 14/104,628.

6.叶勤,李晴,李志敏,吴辉. 利用重组大肠杆菌以甘油为碳源发酵生产琥珀酸的方法. 专利申请号: 201610305322.6.

7.李志敏, 李运杰, 吴辉, 叶勤. 利用乙酸生产丁二酸的代谢工程大肠杆菌菌株构建方法和应用. 专利申请号: 201410599042.1. 已申请PCT专利.

8.孙周通,刘映淼,孙兵兵,王祎,吴辉,杨俊杰等. L-色氨酸基因工程菌,其构建方法以及使用其发酵生产L-色氨酸的方法.ZL201010598350.

9.叶勤,李志敏,吴辉. 利用四碳二元酸发酵固定二氧化碳的方法. ZL200810207564.

10.吴辉,李志敏,叶勤. 大肠杆菌生产琥珀酸的方法. ZL200710135684.


联系方式:

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电话:021-64253701

E-mail: hwu@ecust.edu.cn