王风清
发布人:生工学院网站管理员  发布时间:2016-10-19   动态浏览次数:1460

王风清

  

生物化工专业:硕士生导师

生物工程专业:硕士生导师 

  

   2000年和2003年分别于天津科技大学获学士和硕士学位,2007年毕业于华东理工大学,获生物化工博士学位。长期致力于“微生物代谢工程”相关的研究工作,研究内容涉及:“甾体药物微生物代谢转化合成”、“草本天然产物的微生物合成”、“脂质转化及药物修饰用高值酶”等。近几年,以第一作者或通讯作者在“Metabolic engineering”、“Environmental Science & Technology ” 、“Scientific Reports”、“Applied and Environmental Microbiology”、“Applied Microbiology and Biotechnology”等本领域重点期刊上发表SCI论文多篇。

研究方向:
利用代谢工程和合成生物学的原理和方法,致力于微生物细胞工厂的研究和开发,主要研究内容分为两块:一、针对工业微生物生产领域中的一些关键瓶颈问题,开展生理生化、组学、代谢工程和合成生物学研究,开发高效能微生物菌种。涉及的品种:甾体药物、营养补充剂和有机酸等。二、 针对动植物来源的高值活性天然产物和低产但高附加值的微生物次生代谢产物,开展微生物异源全合成研究。涉及品种:甾体型天然产物、中药活性天然产物、萜类香料和微生物次生代谢产物等。

教育经历:
2003-2007  
华东理工大学,生物工程学院,博士
2000-2003  
天津科技大学,生物工程学院,硕士
1996-2000  
天津科技大学,食品工程系,学士

承担项目:
2014-2017  
国家自然科学基金面上项目  在分枝杆菌中从简单碳源自给自足合成雄甾烯酮的研究 (No. 31370080)
2013-2015  
国家自然科学基金青年项目  细胞壁在Mycolata 类微生物摄取转化甾醇过程中的作用 (No. 21206039)
2013-2014  
企业横向课题  柠檬酸菌株基因工程改造
2011-2013  
中央高校基本科研业务费  甾醇侧链C17-C20键断裂的机理及其基因调控在甾体药物生产过程中的作用
2011-2012   863
项目子课题  柠檬酸、葡萄糖酸的生物转化技术   柠檬酸高品质生产菌株的研发
2008-2010  
自然基金项目  具有新颖催化特征的S. virginiae新菌株降解薯蓣皂素、雌酮等甾类内分泌干扰物的新机制研究 (No. 20777016)
2008-2010   863
项目   基于类固醇药物生产相关重要生物转化反应的工程微生物的研发 (NO. 2008AA02Z209)

产业化项目:
微生物转化甾醇制备甾体医药中间体。
磷脂改性高值产物。

代表论文(* 通讯作者)
1. Li-Qin Xu, Yong-Jun Liu, Kang Yao, Hao-Hao Liu, Xin-Yi Tao, Feng-Qing Wang*, Dong-Zhi Wei. Unraveling and engineering the production of 23,24-bisnorcholenic steroids in sterol metabolism. Scientific Reports, 2016, 6:21928.
2. Xin-Bin Liu, Min Liu, Xin-Yi Tao, Zhong-Xi Zhang, Feng-Qing Wang*, Dong-Zhi Wei. Metabolic engineering of Pichia pastoris for the production of dammarenediol-II. Journal of biotechnology, 2015, 216: 47-55.
3. Kang Yao, Li-Qin Xu, Feng-Qing Wang*, Dong-Zhi Wei. Characterization and engineering of 3-ketosteroid-Δ1-dehydrogenase and 3-ketosteroid-9α-hydroxylase in Mycobacterium neoaurum ATCC 25795 to produce 9α-hydroxy-4-androstene-3,17-dione through the catabolism of sterols. Metabolic Engineering, 2014, 24: 181-191.
4. Hengwei Wang, Fengqing Wang, Wei Wang, Xueling Yao, Dongzhi Wei, Hairong Cheng, Zixin Deng. Improving the expression of recombinant proteins in E. coli BL21 (DE3) under acetate stress: an alkaline pH shift approach. Plos One. 2014, 9(11): e112777.
5. Kang Yao, Feng-Qing Wang*, Huai-Cheng Zhang, Dong-Zhi Wei. Identification and engineering of cholesterol oxidases involved in the initial step of sterols catabolism in Mycobacterium neoaurum. Metabolic Engineering, 2013, 15:75-87.
6. Wei Wei, Shu-Yue Fan, Feng-Qing Wang*, Dong-Zhi Wei. Accumulation of androstadienedione by overexpression of heterologous 3-ketosteroid-C1-dehydrogenase in Mycobacterium neoaurum NwIB-01. World Journal of Microbiology and Biotechnology. 2014, 30: 1947-1954.
7. Miao-Miao Chen, Feng-Qing Wang*, Liang-Cai Lin, Kang Yao, Dong-Zhi Wei. Characterization and application of fusidane antibiotic biosynethsis enzyme 3-ketosteroid-?1-dehydrogenase in steroid transformation. Applied Microbiology and Biotechnology, 2012, 96:133-142.
8. Hengwei Wang, Fengqing Wang, Xinyi Tao Hairong Cheng. Ammonia-containing DMSO: An improved solvent for the dissolution of formazan crystals in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Analytical Biochemistry, 2012, 421:324-326.
9. Liangcai Lin, Weiguo Fang, Xinggang Liao, Fengqing Wang, Dongzhi Wei, Raymond J. St. Leger. The MrCYP52 cytochrome P450 monoxygenase gene of Metarhizium robertsii is important for utilizing insect epicuticular hydrocarbons. PloS One. 2011, 6(12) e28984.
10. Liangcai Lin, Fengqing Wang*, Dongzhi Wei. Chlorimuron ethyl as a new selectable marker for disrupting genes in the insect-pathogenic fungus Metarhizium robertsii. Journal of Microbiological Methods, 2011, 87:241-243.
11. Feng-Qing Wang, Kang Yao, Dongzhi Wei. From soybean phytosterols to steroid hormones. In Hany A. EI-Shemy (ed.) Soybean and Health. Intech, 2011, P231-252.
12. Hengwei Wang, Hairong Cheng, Dongzhi Wei, Fengqing Wang*. Comparison of methods for measuring viable E. coli cells during cultivation: Great differences in the early and late exponential growth phases. Journal of Microbiological Methods, 2011, 84:140-143.
13. Wei Wei, Fengqing Wang*, Shu-yue Fan, Dong-zhi Wei. Inactivation and augmentation of the main 3-ketosteroid- 1-dehydrogenase in Mycobacterium neoaurum NwIB-01: biotransformation of soybean phytosterols to 4-androstene-3,17-dione or 1,4-androstadiene-3,17-dione. Applied and Environmental Microbiology, 2010, 76:4578-4582.
14. Wei Wei, Shuyue Fan, Fengqing Wang*, Dongzhi Wei. A new steroid-transforming strain of Mycobacterium neoaurum and cloning of 3-ketosteroid 9α-hydroxylase in NwIB-01. Applied Biochemistry and Biotechnology. 2010, 162:1446-1456.
15. Bo Li, Wei Wang, Feng-Qing Wang*
Dong-Zhi Wei. Cholesterol oxidase ChoL is a critical enzyme that catalyzes the conversion of diosgnin to 4-ene-3-keto steroids in Streptomyces virginiae IBL-14. Applied Microbiology and Biotechnology, 2010, 85:1831-1838.
16. HengWei Wang, Fengqing Wang, Dongzhi Wei. Impact of oxygen supply on rtPA expression in Escherichia coli BL21 (DE3): ammonia effects. Applied Microbiology and Biotechnology, 2009, 82:249-259.
17. Feng-Qing Wang, Cheng-Gang Zhang, Bo Li, Dong-Zhi Wei, Wang-Yu Tong. New microbiological transformations of steroids by Streptomyces virginiae IBL-14. Environmental Science & Technology. 2009, 43:5967-5974.
18. Wei Wang, Feng-Qing Wang, Dong-Zhi Wei. Characterization of P450 FcpC, the enzyme responsible for bioconversion of diosgnone to isonuatigenone in Streptomyces virginiae IBL-14. Applied and Environmental Microbiology. 2009, 75:4202-4205.
19. Feng-Qing Wang, Bo Li, Wei Wang, Cheng-Gang Zhang, Dong-Zhi Wei.  Biotransformation of diosgenin to nuatigenin-type steroid by a newly isolated strain, Streptomyces virginiae IBL-14. Applied Microbiology and Biotechnology. 2007, 77:771-777.

联系方式:
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办公室:鲁华生物技术研究所401
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E-mail: fqwang@ecust.edu.cn