生物学专业：硕士导师
赵亮 博士
生物与医药专业（生物工程领域）：硕士生导师
生物反应器工程全国重点实验室
细胞与组织工程团队核心成员
个人简介
生物化工博士，副研究员，硕士生导师。美国明尼苏达大学化学工程系著名细胞与代谢工程团队博士后，二十余年细胞培养工程、代谢工程与生物信息学领域科研与产业应用经验，专注于解决产品工程化、产业化的关键科学与技术问题。先后组织了六届细胞培养过程及生物反应器工程高级培训课程和首届细胞培养工程与技术学术年会，协助举办了2014、2015年美国细胞生物过程培训课程和2013~2015年美国生物工程学会CHO细胞系统生物技术联盟培训，多次受邀在国际专业领域会议上做学术报告。近年来发表SCI收录学术论文50余篇、核心期刊论文20篇，获授权发明专利6项。曾受邀Biotech. Bioeng.、Biotech. J、BMC Biotechnology、Process Biochemistry等十余个学术期刊审稿人；连续多年担任国家自然科学基金项目评审专家。先后主持了国家863计划、国家自然科学基金、上海市科研计划等政府项目，以及其他企业开发合作项目。作为主要人员参与了包括国家重大科技专项、国家重大新药创制、国家863重点项目、国家科技支撑计划等一系列重大项目。
研究方向
1.动物细胞大规模培养技术的研究与应用
2.细胞工程与代谢工程技术研究与应用
3.生物技术药物、疫苗、细胞与基因治疗领域相关产品生产技术开发与应用
主持的科研项目或基金：
1. 科技部863计划项目，动物细胞大规模连续灌注培养关键技术及抗体药物研发：无血清培养基高通量开发与优化技术，2012~2015
2. 国家自然科学基金项目，二氧化碳对CHO细胞产物表达行为的影响机制，2012~2014
3. 上海市科委科研计划项目，动物细胞大规模无血清培养及表达产品制备技术，2009~2011
4. 上海市高校青年教师培养资助计划，2011~2013
代表性论文：
1. Liang Zhao, Hsu-Yuan Fu, Weichang Zhou, and Wei-Shou Hu*. Advances in process monitoring tools for cell culture bioprocesses. Engineering in Life Sciences. 2015, 15(5): 459-468
2. Liang Zhao*, Hsu-Yuan Fu, Ravali Raju, Nandita Vishwanathan, Wei-Shou Hu. Unveiling Gene Trait Relationship by Cross-Platform Meta-analysis on Chinese Hamster Ovary Cell Transcriptome. Biotechnology and Bioengineering. 2017, 114(7): 1583-1592
3. Hongping Tang, Shiwei Miao, Xintao Zhang, Li Fan, Xuping Liu, Liang Zhao*, Wen-Song Tan*. Insights into the Generation of Monoclonal Antibody Acidic Charge Variants during Chinese Hamster Ovary Cell Cultures[J]. Applied Microbiology and Biotechnology. 2018, 102(3): 1203-1214
4. Chen Wang, Jiaqi Wang, Min Chen, Li Fan, Liang Zhao*, Wen-Song Tan. Ultra-low carbon dioxide partial pressure improves the galactosylation of a monoclonal antibody produced in Chinese hamster ovary cells in a bioreactor. Biotechnol Lett. 2018, 40(8): 1201-1208
5. Xinning Chen, Jintao Liu, Xuping Liu, Li Fan, Liang Zhao⁎, Wen-Song Tan. Characterization and minimization of sialic acid degradation in an Fc-fusion protein-producing CHO cell bioprocess. Process Biochemistry. 2018, 73: 162-169.
6. Jiaqi Wang, Chen Wang, Li Fan, Liang Zhao*, Wen-song Tan. Simultaneous detection of nicotinamide adenine nucleotides and adenylate pool to quantify redox and energy states in mAb-producing CHO cells by capillary electrophoresis[J]. Analytical and Bioanalytical Chemistry. 2019, 411(13): 2971–2979
7. Weijian Zhang, Xuping Liu, Hongping Tang, Xinran Zhang, Yanan Zhou, Li Fan, Haibin Wang, Wen-Song Tan, Liang Zhao*. Investigation into the impact of tyrosine on the product formation and quality attributes of mAbs in rCHO cell cultures. Applied Microbiology and Biotechnology. 2020, 104(16): 6953–6966
8. Arpan A. Bandyopadhyay, Sofie A. O’Brien, Liang Zhao, Hsu-Yuan Fu, Nandita Vishwanathan, Wei-Shou Hu. Recurring genomic structural variation leads to clonal instabilityand loss of productivity. Biotechnol Bioeng. 2019, 116(1):41-53
9. Christopher S. Stach, Meghan G. McCann, Conor M. O’Brien, Tung S. Le, Nikunj Somia, Xinning Chen, Kyoungho Lee, Hsu-Yuan Fu, Prodromos Daoutidis, Liang Zhao, Wei-Shou Hu, Michael Smanski*. Model-Driven Engineering of N‑Linked Glycosylation in Chinese Hamster Ovary Cells. ACS Synthetic Biology. 2019, 8: 2524-2535.
10. Qian Ye, Thu Phan, Wei-Shou Hu*, Xuping Liu, Li Fan, Wen-Song Tan, Liang Zhao*. Transcriptomic Characterization Reveals Attributes of High Influenza Virus Productivity in MDCK Cells. Viruses. 2021, 13(11), 2200. https://doi.org/10.3390/v13112200.
11. Yanmin Zhang, Weijian Zhang, Jun Cheng, Xuping Liu, Shiwei Miao, Wen-Song Tan, Liang Zhao*. Designing a novel E2-IFN-γ fusion protein against CSFV by immunoinformatics and structural vaccinology approaches. Applied Microbiology and Biotechnology. 2022, 106(9-10): 3611-3623
12. Qian Ye, Xuping Liu, Yuxiang Wan, Wen-Song Tan, Liang Zhao*. Metabolic reprogramming and alteration of the redox state in hyper-productive MDCK cells for influenza a virus production. Biologicals. 2022, 80:35-42
13. Yanmin Zhang, Daoyuan Na, Weijian Zhang, Xuping Liu, Shiwei Miao, Wen-Song Tan, Liang Zhao*. Development of stable HEK293T cell pools expressing CSFV E2 protein: A potential antigen expression platform. Vaccine. 2023, 41(9):1573-1583
14. Weijian Zhang, Qingyuan Ran, Liang Zhao, Qian Ye*, Wen-Song Tan. Characterization of cellular responses and cell lysis to elevated hydrodynamic stress from benchtop perfusion bioreactors. Biotechnol J. 2024, 19(3): e2400063
15. Chenxi Gao, Weijian Zhang, Liang Zhao*, Wen-Song Tan. A novel approach for perfusion process design based on a Grey-Box kinetic model. BIOPROCESS AND BIOSYSTEMS ENGINEERING. 2024, 47(12): 2011-2025
授权发明专利：
1. 牛红星, 赵亮, 范里, 周燕, 谭文松. 适于NS0细胞大规模培养及抗体生产的无蛋白培养基. 专利号: ZL200810039305.8. 2012-1-4授权
2. 谭文松, 赵亮, 周燕, 陈飞. 适于动物细胞产品生产的无动物来源低蛋白培养基. 专利号: ZL200910053506.8. 2011-1-19
3. 谭文松, 赵亮, 周燕, 黄锭. 适于MDCK细胞大规模贴壁培养和单细胞悬浮培养的无血清培养基. 申请号: 201010022834.4. 2012-6-27授权
4. 谭文松, 赵亮, 范里, 刘旭平, 周燕, 孙亚婷. 适于动物细胞高密度培养和产物表达的营养物添加剂. 申请号: CN201110148826.9. 2013-1-23授权
5. 谭文松，孙亚婷，赵亮，范里，刘旭平，唐红萍，邓献存. 适于动物细胞表达产品大规模生产的化学成分明确培养基. 申请号：201410513086.8, 授权公告号：CN104328158B. 授权公告日：2017.11.24
6. 谭文松，刘旭平，赵亮，范里，黄锭. 适于大规模生产流感疫苗的无血清培养基. 申请号：CN201310011998.0. 20140611授权
联系方式：
上海市徐汇区梅陇路130号华东理工大学
邮编：200237
办公室：实验18楼1015室
电话：021-64253394
邮箱：zhaoliang@ecust.edu.cn