阳大海
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阳大海

  

生物工程专业:硕士生导师

  

生物工程领域:工程硕士导师

   

    博士,副教授,硕士生导师。华东理工大学和美国密西根大学联合培养博士,2015年毕业后留校,2018年晋升副教授,现为生物反应器工程国家重点实验室固定人员。先后入选中国科协 青年人才托举工程、上海市浦江人才计划晨光人才计划、华东理工大学青年英才培育计划。主持国家自然科学基金,参与国家重点研发计划等项目。以第一/通讯作者在ImmunityNature CommunicationsPLoS Pathogens等领域内高水平期刊发表研究论文10余篇,以共同作者在NatureCellCell Host & Microbe等期刊发表论文15篇,被SCI刊源引用 550余次;担任Microbial Cell杂志编委,为Journal of Immunology, Communications BiologyScientific Reports, JoVE, Fish & Shellfish Immunology等领域内SCI杂志审稿30余篇,在国内外学术会议做报告20余次。

 

Short Biography:

Dahai Yang obtained his Ph.D. from East China University of Science and Technology (ECUST) in 2015. Prior to returning to China to assume an Assistant Professor at ECUST, he was a joint PhD student with Dr. Gabriel Núñez from the University of Michigan (2013-2015). In 2018, he was promoted to become an Associate Professor at ECUST. He awards the Young Elite Scientists Sponsorship Program, Shanghai Pujiang Program and Shanghai Chenguang Program. Dr. Yang’s research spans from bacterial pathogenesis to pyroptotic cell death and to septic-organ dysfunction. In mouse model, he has identified the Pannexin-1 and P2x7 receptor axis as the key substrates downstream of Caspase-11 during cytosolic LPS infection in macrophages. In zebrafish model, he has uncovered the role of bacterial haemolysin in promoting outer membrane vesicles (OMV)-contained LPS releasing into cytosol to activate pyroptotic‐like cell death in non-phagocyte cells, and identified Caspy2 as the cytosolic pattern recognition receptor for LPS. His research is supported by grants from the National Natural Science Foundation of China and China Association for Science and Technology.

 

Research Interests:

Dr. Dahai Yang’s group is interested in fundamental research in microbial-host interactions, innate immune responses, the pathogenesis of inflammatory disease. Specifically, the research focuses on mechanistic studies to understand the role of bacterial infection-induced fish pyroptosis in regulating multi-organ dysfunction during sepsis. Several approaches that include biochemical reconstitution, cell biology, analysis of genetically modified mutant mice, and zebrafish genetics to identify new components in pathogen-induced inflammasome activation and to further reveal the underlying biochemical mechanism involved in the interaction between microbial/endogenous molecules and sepsis.

 

教育/工作经历:

2018.09-              华东理工大学生物工程学院     副教授,硕士生导师

2015.12-2018.08     华东理工大学生物工程学院     讲师

2013.08-2015.05     美国密西根大学医学院            联培博士,合作导师:Gabriel Nunez

2010.09-2015.06     华东理工大学生物工程学院     博士,导师:刘琴/张元兴

 

人才计划/科研项目:

1.        中国科协第二批青年人才托举工程 2016QNRC001

2.        国家自然科学基金青年项目(31602187

3.        教育部高校基本科研业务费探索基金(222201714022

4.        上海市 浦江人才计划D类)(2016PJD020

5.        上海市晨光计划A类)(16CG33

6.        华东理工大学青年英才培育计划(2018-2021

7.        生物工程学院青年英才远志计划2016-2018

 

主要研究方向: 

1. 细菌感染激活细胞焦亡分子机制

2. 脓毒症多器官损伤发生机制

3. 鱼类抗感染免疫分子机制

 

研究概述:

我们的研究兴趣集中在细菌感染激活细胞焦亡与脓毒症发生分子机制。以小鼠/斑马鱼/海水养殖经济鱼种为模型,从宿主抗感染天然免疫信号通路入手,致力于解析感染性多器官损伤/脓毒症的关键免疫机制,取得了一系列创新性进展:(1鉴定了细菌LPS诱导巨噬细胞炎症性坏死信号通路的关键分子。采用内毒素转染巨噬细胞,建立了非经典炎症小体激活模型,阐明了炎症性Caspase底物Pannexin-1 P2x7 受体信号在调控细胞焦亡过程中发挥的作用,为治疗革兰氏阴性细菌引发的脓毒症休克提供了新的理论支撑(Immunity 2015, 43: 923)。研究成果受到了Immunity的亮点评述和推荐,并在发表后受到了Nat Rev ImmunolCellF1000Research 等领域内重要综述引用报道130余次。(2解析了细菌溶血素促进内毒素释放并激活非经典炎症小体分子机制。发现了溶血素促进细菌外膜囊泡携带LPS进入细胞质,激活非免疫细胞焦亡的分子机制(Cellular Microbiology 2019, e13010,封面论文)。(3以斑马鱼为模型,确定了模式识别演变中的关键事件。鉴定了细菌LPS在斑马鱼中的胞内天然免疫受体Caspy2,解析了先天免疫中死亡结构域超家族介导细胞内LPS感应途径(Nature Communications 2018, 9: 3052)。(4建立了斑马鱼脓毒症发生究模型。阐明了细胞焦亡信号调控脓毒症急性肾损伤发生的分子机制,为该疾病的靶向药物筛选提供了新的研究平台。上述研究工作对理解革兰氏阴性细菌感染的致病机理有极大的促进作用,获得的功能分子或病害防治靶标为感染引发的系统性疾病的免疫防控提供了新思路。

目前,我们对细菌感染激活细胞焦亡信号如何调控脓毒症多器官损伤的分子机制感兴趣,将结合流行病学、病原微生物学、生物化学、细胞生物学、以及小鼠和斑马鱼遗传学等多种手段来研究和解析细菌感染激活炎症小体引发细胞焦亡的分子机制,阐明其调控器官功能损伤、诱导感染性疾病发生的病理生理机制。

 

发表论文 (* Corresponding Author, # Co-first Author):

2019:

Yang D.#, Liu X. #, Xu W., Gu Z., Yang C., Zhang L., Tan J., Zheng X., Wang Z., Quan S., Zhang Y., Liu Q.*. The Edwardsiella piscicida thioredoxin-like protein inhibits ASK1-MAPKs signaling cascades to promote pathogenesis during infection, PLoS Pathogens 2019, 15(7): e1007917. (IF=6.4)

Wen Y., Chen S., Jiang Z., Wang Z., Tan J., Hu T., Wang Q., Zhou X., Zhang Y., Liu Q., Yang D.*, Haemolysin promotes bacterial outer membrane vesicles-induced pyroptotic-like cell death in zebrafish, Cellular Microbiology 2019, e13010.  (IF=4.41)

Wang Z., Lin L., Chen W., Zheng X., Zhang Y., Liu Q., Yang D.*, Neutrophil plays critical role during Edwardsiella piscicida immersion infection in zebrafish larvae. Fish and Shellfish Immunology 2019, 87: 565-572. (IF=3.185)

Tan J., Yang D., Wang Z., Zheng X., Zhang Y., Liu Q.*. EvpP inhibits neutrophils recruitment via Jnk-caspy inflammasome signaling in vivo. Fish and Shellfish Immunology 2019, 92: 851-860.  (IF=3.185)

Hu T, Zhang L, Wang W, Yang D, Xiao J, Zhang Y, Liu X, Liu Q*. Edwardsiella piscicida enters nonphagocytic cells via a macropinocytosis-Involved hybrid mechanism. Journal of Bacteriology 2019, 201 (5): e00548-18. (IF=3.219)

2018:

Yang D., Zheng X., Chen S., Wang Z., Xu W., Tan J., Hou M., Wang W., Gu Z., Wang Q., Zhang R., Zhang Y., Liu Q.*, Sensing of cytosolic LPS through caspy2 pyrin domain mediates noncanonical inflammasome activation in zebrafish, Nature Communications 2018, 9: 3025. (IF=12.353)

Cao H., Yang C., Quan S., Hu T., Zhang L., Zhang Y., Yang D.*, Liu Q.*, Novel T3SS effector EseK in Edwardsiella piscicida is chaperoned by EscH and EscS to express virulence, Cellular Microbiology 2018, 20 (1): e12790.  (IF=4.41)

Cao H., Han F., Tan J., Hou M., Zhang Y., Yang D.*, Liu Q.*, Edwardsiella piscicida T3SS effector EseK inhibits MAPKs phosphorylation and promotes bacterial colonization in zebrafish larvae, Infection and Immunity 2018, 68 (9): e00233-18. (IF=3.63)

Xu W., Gu Z., Zhang L., Zhang Y., Liu Q., Yang D.*, Edwardsiella piscicida virulence effector trxlp promotes the NLRC4 inflammasome activation during infection, Microbial Pathogenesis 2018, 123: 496-504.  (IF=2.332)

Hara H., Seregin SS., Yang D., Fukase K., Chamaillard M., Emad SA., Inohara N., Chen G.Y, Nunez G.*, NLRP6 recognizes lipoteichoic acid and activates caspase-11 and caspase-1 to regulate Gram-positive pathogen infection. Cell 2018, 18: 31259-5 (IF=28.71)

 Chen S., Yang D., Wen Y., Jiang Z., Zhang L., Jiang J., Chen Y., Hu T., Wang Q., Zhang Y., Liu Q.*, Dysregulated hemolysin liberates bacterial outer membrane vesicles for cytosolic lipopolysaccharide sensing. PLoS Pathogens 2018, 14(8): e1007240 (IF=6.158)

Zhang L., Jiang Z., Fang S., Huang Y., Yang D., Wang Q., Zhang Y., Liu Q.*, Systematic identification of intracellular-translocated candidate effectors in Edwardsiella piscicida, Front Cell Infect Microbiol. 2018, 8:37. (IF=4.855)

Hou M., Wang W., Hu F., Zhang Y., Yang D., Liu Q.*, Phosphothreonine lyase promotes p65 degradation in a mitogen-activated protein kinase/mitogen- and stress-activated protein kinase 1-dependent manner, Infection and Immunity 2018, 87(1): e00508-18. (IF=3.63)

 Li M., Xu Y.*, Sun J., Wang M., Yang D., Guo X., Song H., Cao S., Yan Y., Fabrication of charge-conversion nanoparticles for cancer imaging by flash nanoprecipitation. ASC Appl Mater Interfaces 2018, 10: 10752-10760. (IF=8.097)

 Wang M., Xu Y.*, Liu Y., Gu K., Tan J., Shi P., Yang D., Guo Z., Zhu W., Guo X., Cohen Stuart MA., Morphology tuning of aggregation-induced emission probes by flash nanoprecipitation: Shape and size effects on in vivo imaging. ASC Appl Mater Interfaces 2018, 10: 25186-25193. (IF=8.097)

 He H., Liu Y., Zhou Z., Guo C., Wang HY., Wang Z., Wang X., Zhang Z., Wu FG., Wang H., Chen D., Yang D., Liang X., Chen J., Zhou S., Liang X., Qian X., Yang Y.*, A photo-triggered and photo-calibrated nitric oxide donor: rational design, spectral characterization, and biological applications, Free Radic Biol Med. 2018, 123: 1-7. (IF=6.020)

 He H., Xiao Y., Qi Y., Wang HY., Wang Z., Bao J., Zhang Z., Wu FG., Wang H., Chen D., Yang D., Liang X., Chen J., Zhou S., Liang X., Qian X., Yang Y.*, A water-soluble, green-light triggered, and photo-calibrated nitric oxide donor for biological applications. Bioconjug Chem. 2018, 29: 1194-1198. (IF=4.485)

2017:

Chen H., Yang D., Han F., Tan J., Wang Q., Zhang Y., Liu Q.*, Bacterial T6SS effector EvpP prevents NLRP3 inflammasome activation by inhibiting Ca2+-dependent JNK pathway, Cell Host & Microbe 2017, 21: 47-58. (IF=17.872)

Hou M, Chen R., Yang D., Núñez G., Wang Z., Wang Q., Zhang Y., Liu Q.*, Identification and functional characterization of EseH, a new effector of the type III secretion system of Edwardsiella piscicida, Cellular Microbiology 2017, 19: e12638.  (IF=4.41)

2016:

He Y., Zeng M.Y., Yang D., Motro B., Núñez G.*, Nek7 is an essential mediator of NLRP3 activation downstream of potassium efflux. Nature 2016, 530(7590): 354-7. (IF=41.577)

Zhang L., Ni C., Xu W., Dai T., Yang D., Wang Q. Zhang Y., Liu Q.*, Intra-macrophage infection reinforces the virulence of Edwardsiella tarda, Journal of Bacteriology 2016, 198 (10): 1534-1542. (IF=3.219)

Fang S., Zhang L., Lou Y., Yang D., Wang Q., Zhang Y., Liu Q.*, Intracellular translocation and localization of Edwardsiella tarda type III secretion system effector EseG in host cells, Microbial Pathogenesis 2016, 97: 166-171. (IF=2.332)

2015 & before:

Yang D., He Y., Muñoz-Planillo R., Liu Q.*, Núñez G.*. Caspase-11 requires the pannexin-1 channel and the purinergic P2X7 pore to mediate pyroptosis and endotoxic shock. Immunity 2015, 43: 923-932.  (IF=24.082)

Previewed by: Aude de Gassart and Fabio Martinon*. Pyroptosis: Caspase-11 unlocks the gates of death. Immunity 2015, 43: 835-837.

Yang D., Liu Q.*, Ni C., Li S., Wu H., Wang Q., Xiao J., Zhang Y., Gene expression profiling in live attenuated Edwardsiella tarda vaccine immunized and challenged zebrafish: Insights into the basic mechanisms of protection seen in immunized fish, Developmental and Comparative Immunology 2013, 40: 132-141.  (IF=3.25)

Yang D., Liu Q.*, Yang M, Wu H., Wang Q., Xiao J., Zhang Y.*, RNA-seq liver transcriptome analysis reveals an activated MHC-I pathway and an inhibited MHC-II pathway at the early stage of vaccine immunization in zebrafish, BMC genomics 2012, 13: 319. (IF=3.620)

Highly accessed article in BMC genomics (online 30 days).

 

联系方式:

联系地址:上海市梅陇路130 华东理工大学 阿华生物工程研究所101

办公电话:021-64253065

E-maildahaiyang@ecust.edu.cn