马兴元
发布人:生工学院网站管理员  发布时间:2016-10-19   动态浏览次数:2035

 

马兴元

  

生物化学与分子生物学专业:博士生导师/硕士生导师
    生物工程专业:博士生导师/硕士生导师

微生物学专业:硕士生导师

生物工程:工程硕士生导师

  

男,博士,教授/博导,上海市“浦江学者”,华东理工大学 生物反应器国家重点实验室学 固定研究人员、 生物药学实验室/课题组负责人。1995年7月和1999年7月从山西农业大学和沈阳农业大学毕业,先后获得动物医学专业学士学位和硕士学位;2003年6月毕业于中国人民解放军军需大学(现吉林大学)获博士学位,师从殷震院士、朱平研究员;硕士与博士期间在军需大学研究所 (军事医学科学院 第11所) 全军基因工程重点实验室从事分子生物学与基因工程药物的研究。2003年博士毕业后在华东理工大学生物工程博士后流动站从事博士后研究工作;2010.12—2011.12在新加坡国立大学(MIT-NUS联合所)国家公派(A类)访问学者。


研究领域  分子生物学与生物技术药物/生物制品

研究方向重组蛋白新药(多肽/疫苗/抗体)及稳定与靶向转载制剂;②微生物分子免疫学及人和动物疫病免疫制品与生物防治。③细胞基因组编辑技术与蛋白质(酶)工程及生物合成;


学术兼职及荣誉奖励Drug Discoveries & Therapeutics编委;Chinese Journal of Biology编委;Science,Technology and Development编委;《中华现代临床医学杂志》常务编委;《中国医药导报杂志》编委;《中国细胞生物学学报》、《中国生物工程杂志》特约Reviewer;中国生化与分子生物学会工业生化与分子生物学分会 常务理事;中国药学会 生化与生物技术药物专业委员会 委员; 中国蛋白药物质量与技术联盟 理事、第一届质量专家委员会 委员;The Society for Biological Engineering (SBE)、中国/上海生物工程学会会员。 部分奖励13届国际生物技术大会(2008年)国际生物技术青年科学家奖;上海市生物工程学会(2009)青年科学家学术年会暨纪念刘新垣院士80周年论文一等奖;中国生物工程学会学术年会(2015年)“GE生命科学”优秀论文一等奖;上海市28届优秀发明选拔赛三等奖(第1)(铜奖)、二等奖(第4)银奖(2015);上海市2016年科技发明(生物医药组)三等奖(第5)。


承担科研项目

(1)国家自然科学基金项目(主持:30873190、 31670944)、 (参与:81673345、 30870253、31300660);

(2)国家科技部与卫计委“新药创制”重大专项(主持:2009ZX09103-693、2015GKS-470[遴选入库跟踪项目])、(参与:2009ZX09306-008、2004AA2Z3801 [863计划]);

(3)教育部交叉学科与重大项目培育基金重点项目(主持:WK0913002)、教育部博士点专项基金(主持:20070251011)、教育部新教师科研专项基金(参与:20120074120027);

(4)上海市项目:“浦江学者”人才计划项目(主持:13PJD012)、 上海市自然科学基金(主持:05ZR14033)、上海市国家新药重大专项配套项目(主持:2009ZX09103-693)、上海市科委生物医药科技重点项目(主持:054319928、参与:14431904300)、上海市博士后基金(10R21412000);

(5)其它项目主持生物反应器工程国家重点实验室面上项目、国际开放合作项目(新加坡)、国内开放合作项目(厦门大学)、华东理工大学引进人才启动基金项目,及主持完成军队课题2项。


学术论文:

1.Xingyuan Ma, Nanjing Lin, Yanyan Kang, Linfeng Li*, Wenyun Zheng*. Screening and identification of chicken BMSCs-specific McAbs from hybridoma cell lines used for discovering novel biomarkers of BMSCs. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 2016,35(4):199-211

2.Zengjie Zheng, Hailong Jiang, Yan Huang, Jie Wang, Lei Qiu, Zhenlin Hu,Xingyuan Ma* & Yiming Lu*. Screening of an anti-inflammatory peptide from Hydrophis cyanocinctus and analysis of its activities and mechanism in DSS-induced acute colitis. Scientific Reports, 2016, 25672, DOI: 10.1038/srep25672.

3.Hui Liu, Xiaodan Cao, Ping Wang*, Xingyuan Ma*. pH-sensitive pHluorins as a molecular sensor for in situ monitoring of enzyme-catalyzed prodrug activation. Biotechnology and Applied Biochemistry, 2016 May 25. doi: 10.1002/bab.1511

4.Hua Guo, Na Zhang, Di Liu, Ping Wang*, Xingyuan Ma*. Cell permeable manganese superoxide dismutase (TAT-MnSOD) induced inhibitory effect on the proliferation of human heptoma. Biomed Pharmacother. 2016, 29(83):1379-1386.

5.Xingyuan Ma, Yi Zhang, Yanyan Kang, Linfeng Li, Wenyun Zheng. A recombinant protein TmSm(T34A) can inhibit proliferation and proapoptosis to breast cancer stem cells(BCSCs) by down-regulating the expression of Cyclin D1.Biomed Pharmacother. 2016, 23(84):373-381.

6.Na Zhang, Hua Guo, Wenyun Zheng, Tianwen Wang, Ping Wang, Xingyuan Ma*. Design and screening of a chimeric survivin-specific nanobody and its anticancer activities in vitro. Anti-Cancer Drugs. 2016, 27(9):839-47.

7.Na Zhang, Hua Guo, Wenyun Zheng, Ping Wang, Xingyuan Ma*.The small and powerful nanobodies (Nbs) originating from peculiar animals used for therapy of cancers.International Journal of Cancer Studies & Research. 2016, 4: 1-7.

8.Di Liu,Hua Guo, Wenyun Zheng, Na Zhang, Tianwen Wang, Ping Wang, Xingyuan Ma*.Discovery of the cell-penetrating function of A2 domain derived from LTA subunit of Escherichia coli heat-labile enterotoxin. Applied Microbiology and Biotechnology.2016:6(3):1-10

9.Di Liu, Na Zhang, Wenyun Zheng, Hua Guo, Xiaoli Wang, Tianwen Wang, Ping Wang, Xingyuan Ma*. A novel method of efficient preparation to mucosal adjuvant E. coliheat-labile enterotoxin mutant (LTm) by artificial self-assembly in vitro. Applied Biochemistry and Biotechnology. 2016, 2(15):11-13.

10.Guoying Dai, Wenyun Zheng, Xingyuan Ma*, Ping Wang*.Multisite Mutation of Monomer Survivin with Enhanced Effect on Apoptosis Regulation of Breast Cancer Cells. Biomed Pharmacother.2015, 69(2):111-118.

11.Fei Ma, Tianwen Wang, Xingyuan Ma*, Ping Wang*. Identification and Characterization of the Protein Encoded by orf382 as an L-Threonine Dehydrogenase. J. Microbiol. Biotechnol. 2014, 24(6): 748–755.

12.Nanjing Lin, Wenyun Zheng, Linfeng Li, Ping Wang, Xingyuan Ma*, A novel system enhancing the endosomal escapes of peptides promotes Bak BH3 peptide inducing apoptosis in lung cancer A549 cells. Targeted Oncology. 2014, 9(2): 163-170.

13.Tianwen Wang, Xingyuan Ma*, Hu Zhu, Aitao LI, Guocheng Du, Jian Chen. Available methods for assembling expression cassettes for synthetic biology. Applied Microbiology and Biotechnology. Appl Microbiol Biotechnol. 2012 93(5): 1853–1863.

14.Li Hui, Zhang Qian, Wang Xiao li, Ma Xing yuan*, Lin Kuang fei*, Liu Yong di, Gu Ji dong. Biodegradation of benzene homologues in contaminated sediment of the East China Sea. Bioresource Technology, 2012, 124: 129-136.

15.Yuxin Xu, Wenyun Zheng, Tianwen Wang, Ping Wang, Ling Zhu, Xingyuan Ma*. Genetic protein TmSm(T34A) enhances sensitivity of chemotherapy to breast cell lines as a synergistic drug to doxorubicin. Biomedicine & Pharmacotherapy, 2012, 66 (5) : 368-372.

16.Hailong Chen,Xingyuan Ma*, Zhi Li, Qiaoyun Shi, Wenyun Zheng, Yang Liu, Ping Wang. Functionalization of single-walled carbon nanotubes enables efficient intracellular delivery of siRNA targeting MDM2 to inhibit breast cancer cells growth. Biomedicine & Pharmacotherapy, 2012, 66 (5):334-338.

17.Tianwen Wang, Xingyuan Ma*, Guocheng Du, Jian Chen. Overview of Regulatory Strategies and Molecular Elements in Metabolic Engineering of Bacteria. Mol Biotechnol. 2012, 52(3): 300-308.

18.Tianwen Wang, Aitao Li,Xingyuan Ma*, Guocheng Du, Jian Chen.. A simple and universal method to express protein in unfused form. Protein Pept Lett. 2012, 19(9): 930-933.

19.Wenyun Zheng, Yanyan Kang, Linfeng Li, Yuxin Xu,Xingyuan Ma*. Levels of effectiveness of gene therapies targeting survivin and its splice variants in human breast cancer cells. Drug Discoveries & Therapeutics. 2011; 5(6):293-298.

20.Xingyuan Ma, Bi Yao, Wenyun Zheng, Linfeng Li*. Comparative study on characterization of recombinant B subunit of E. coli heat-labile enterotoxin (rLTB) prepared from E. coli and P. patoris. J. Microbiol. Biotechnol. 2010, 20(3):550-557.

21.Haiyi Zhang, Yu Zheng, QinghaiLiu, Xinyi Tao, Wenyun Zheng,Xingyuan Ma*, Dongzhi Wei*. Development of a fed-batch process for the production of anticancerdrug TATm-survivin(T34A) in Escherichia coli.Biochemical Engineering Journal. 2009, 43(2):163–168.

22.Li Zhao, Yu Zheng, Xingyuan Ma*and Dongzhi Wei*. Effects of over-expression of glycerol dehydrogenase and 1,3-propanediol oxidoreductase on bioconversion of glycerol into 1, 3-propandediol by Klebsiella pneumoniae under micro-aerobic conditions. Bioprocess and Biosystems Engineering, 2009, 32(3): 313-320.

23.Xingyuan Ma, Meiyun Liu, Yushu Ma, Yi Gong *, Dongzhi Wei*, Shengli Yang. Disulfide bond Cys30-Cys299 and the fragment “96RIMQAPLT106”of the lid region of lip2 are critical for its biocatalytic activity. Journal of Biotechnology. 2008, S290-334.

24.Xingyuan Ma*, Wenyun ZhengHaiyi ZhangXinyi TaoQinghai LiuDongzhi Wei. Large scale preparation and characterization of a genetically engineering protein and biological activity of suppressing proliferation and rapid pro-apoptosis to carcinoma cells. Journal of Biotechnology. 2008, S75-S98.

25.Xingyuan Ma, Wenyun Zheng, Dongzhi Wei, Yushu Ma, Tianwen Wang, Jinzhi Wang, Qinghai Liu and Shengli Yang. Construction, Expression, and Purification of HIV-TAT-survivin (T34A) mutant: a Pro-apoptosis protein in E.coli. Protein Expression and Purification, 2006, 47:36-44.

26.Xingyuan Ma, Jianhua Tan*, Dongzhi Wei, Pin Zhu, Manji Sun. High-level secretion and purification of recombinant acetylcholinesterase from human brain in P. pastoris and identification by chromogenic reaction. Applied Microbiology and Biotechnology. 2006, 72: 316-322.

27.Ma Xingyuan, Zheng Wenyun, Wei Dongzhi, Ma Yushu, Wang Tianwen, Wang Jinzhia, Liu Qinghai, Yang Shengli, High-level Expression, Purification and Pro-apoptosis Activity of HIV-TAT-Survivin (T34A) Mutant to Cancer Cells in Vitro, Journal of Biotechnology, 2006, 123:367-378.

28.Xingyuan Ma, Wenyun Zheng, Tianwen Wang, Dongzhi Wei, Yushu Ma. Optimization and high-level expression of a functional GST-tagged rHLT-B in E. coli and GM1 binding ability of purified rHLT-B. Journal of Microbiology,2006, 44(6): 293-300.

29.Xingyuan Ma, Zheng Wenyun, Tianwen Wang. Leukocyte function-associated antigen-1 structure, function and application prospects. Protein and Peptide Letters, 2006, 13: 397-400.

30.Tianwen David Wang & Fei Ma & Xingyuan Ma & Ping Wang*. Spatially programmed assembling of oxidoreductases with single-stranded DNA for cofactor-required reactions. Appl Microbiol Biotechnol.2015,99(8):3469-3477

31.Liqun Wang, Yanyan Kang, Wenyun Zheng*, Linfeng Li, Lei Shi, Xingyuan Ma. Effect on apoptosis and cell cycle of recombinant double negative 4 dominant mutation Survivin (T34/117A) in breast cancer cell B-Cap-37. Biomedicine & Pharmacotherapy, 2014, 68(3): 277–284.

32.Rijun Gui, Xueqin An, Hongjuan Su, Weiguo Shen, Linyong Zhu, Xingyuan Ma, Zhiyun Chen, Xiaoyong Wang. Rhodamine 6G conjugated-quantum dots used for highly sensitive and selective radiometric fluorescence sensor of glutathione. Talanta, 2012, 94 (5) 295-300.

33.Dan Qiu, Xueqin An, Zhiyun Chen, Xingyuan Ma. Microstructure study of liposomes decorated by hydrophobic magnetic nanoparticles. Chemistry and Physics of Lipids. 2012,165 (5):563-570.

34.Li Zhao, XingyuanMa, Yu Zheng, Jianguo Zhang, Guodong Wei, Dongzhi Wei. Over-expression of glycerol dehydrogenase and 1, 3-propanediol oxidoreductase in Klebsiella pneumoniae and their effects on conversion of glycerol into 1, 3-propanediol in resting cell system. Journal of Chemical Technology & Biotechnology. 2009, 84(4): 626-632.

35.Yu Zheng, Li Zhao,Xingyuan Ma, Dongzhi Wei*. Production of 1, 3-propanediol from glucose by co-expressing glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase in Klebsiella pneumoniae. Journal of Bioscience and Bioengineering, 2008, 105 (5): 508–512.

36.Yu Zheng, Li Zhao, Haiyi ZhangLiujin Wei,Xingyuan Ma, Dongzhi Wei*.One-step production of 2,3-butanediol from starch by secretory over-expression of amylase in Klebsiella pneumoniae. Journal of Chemical Technology & Biotechnology. 2008, 83: 1409-1412.

37.Qinghai Liu, Jinping Lin, Meiyun Liu, Xinyi Tao, Dongzhi Wei, Xingyuan Maand Shengli Yang Large scale preparation of recombinant human parathyroid hormone 1–84 from Escherichia coli. Protein Expression and Purification, 2007,54: 212-219.

38.Tianwen Wang, Hu Zhu, Xingyuan Ma, Yushu Ma and Dongzhi Wei. Mutant library construction in directed molecular evolution: casting a net wider. Mol Biotechnol. 2006, 34(1): 55-68.

39.Tianwen Wang, Hu Zhu, Xingyuan Ma, Yushu Ma and Dongzhi Wei. Structure-based stabilization of an enzyme: the case of penicillin acylase from Alcaligenes faecalis. Protein Peptide Letters, 2006, 13 (2):177-183.

40.Tianwen Wang, Hu Zhu, Xingyuan Ma, Zhuoya Fei, Yushu Ma and Dongzhi Wei. Enhancing enzymatic activity of penicillin G acylase by coexpressing pcm gene. Applied Microbiology and Biotechnology, 2006, 72(5): 953-958.

41.Zheng Wenyun, Ma Xingyuan, Wei Dongzhi, Ma Yushu, Wang Tianwen, Wang Jinzhia, Liu Qinghai, Yang Shengli. Preparation of recombinant HIV-TATm-survivin (T34A) protein and its pro-apoptosis activity to four cancer cell lines in Vitro, Chinese Journal of Biotechnology, 2006.22(2): 285-292.

42.Wenyun Zheng, Xingyuan Ma, Dongzhi Wei, Tianwen Wang, Yushu Ma, Shengli Yang. Molecular cloning and bioinformatics analysis of a novel spliced variant of survivin from human breast cancer cells, DNA Sequence, 2005, 16 (5): 321-328.

43.张旭凡,胡发彪,马兴元*,王天文,王平,王晓丽。由乙肝病毒核心蛋白(HBc-VLP)递载的流感通用疫苗Flu@uV设计构建、表征及初步活性研究。中国生物工程杂志,2016,36(2):7-15.

44.郑文云, 马兴元, 魏东芝,王锦之,刘清海,马昱澍,杨胜利。重组HIV-TATm-Survivin(T34A)蛋白制备及其促癌细胞凋亡活性研究. 生物工程学报2006,22(2):285-292.

45.姚碧,刘琨,陈海龙,马兴元*。组成新发典型甲型H1N1流感病毒八个基因片段的变异分析与疫病流行原因初探。 中国兽医科学,2009,39(8):679-684.

46.刘琨,陈海龙,姚碧,马兴元*。 新发甲型H1N1流感病毒HA分子的变异分析。 现代生物医学进展,2009,9(20):3801-3806.

47.马兴元。 细胞分裂与凋亡的重要调节者Survivin:一个抗癌新药靶。生命的化学,2010,30(3):338-344.

48.刘琨,陈海龙,马兴元*,康燕燕,许玉馨。酵母Bir1p同源物Survivin对毕赤酵母的分子重组与生长影响。生物技术通报,2010,220(11):230-233.

49.康燕燕,唐莉莉,石磊,陈海龙,马兴元*。重组蛋白TATm-Survivin(T34/117A)对乳腺癌细胞周期变化与凋亡的影响。中国癌症杂志,2011,22(2):91-98.

50.张翼,李林凤,马兴元*。细胞培养环境中4 种常见基础性因子对乳腺癌细胞生长的影响。 中国科技论文2011.11在线发表 (http://www.paper.edu.cn).

51.朱玲,石磊,唐莉莉,许玉馨,张翼,马兴元*TATm-Survivin(T34A)冻干注射剂的制备及体外抗肿瘤效果。中国新药杂志,2012,21(17): 15-19.

52.石磊,唐莉莉,马兴元*,王天文,马飞,王平。利用类弹性蛋白可逆相变和内含肽自切割功能高效制备TmSm抗肿瘤蛋白。中国生物工程杂志,2013,33(10):89-95.

53.马兴元。非小细胞肺癌治疗性疫苗及相关生物标志物研发进展。生物产业技术,2013,5(9)22-29.

教材/著作:

1.主编, 《抗体工程》,华东理工大学出版社,在编;

2.主编,《蛋白质与酶工程》,化学工业出版社,在出版中;

3.参编,《免疫学原理与技术》(iCourse教材·生物技术与生物工程系列),高等教育出版社,ISBN 9787040455687出版时间:2016年9月;

4.主编,《疫苗工程》(国家十一五规划重点教材),华东理工大学出版社, ISBN:9787562825845出版时间:2009年9月;

5.主译,《生物催化剂与酶工程》, 科学出版社,ISBN:9787030221957 出版时间:2008.9;

6.编译审校,《生物技术和遗传工程词汇》(The FAO Biotechnology Glossary)(2010)联合国粮农组织(FAO)ISBN:1020-0541;

7.主编,《国家法定猪病诊断与防制》,中国轻工业出版社,ISBN:9787501958047出版时间:2007.6;

8.副主编,《国家法定禽病诊断与防制》,中国轻工业出版社,ISBN:7501942722出版时间:2005.4;

9.副主编,《奶牛养殖顾问》辽宁科技出版社,出版时间:2004;

10.审校,《面向21世纪课程教材—兽医传染病学》,中国农业大学出版社,出版时间:2002;


发明专利:

1.名称:大肠杆菌热敏毒素B亚单位的真核表达方法,授权号:ZL200510027957.6

2.名称:一种用于原核分泌融合表达大肠杆菌热敏毒素B亚单位的载体,授权号:ZL200510028084.0

3.名称:含有HIV转导结构域的生存素突变体及制备方法和应用,授权号:ZL200510026847.8

4.名称: 一种肿瘤化疗药物的蛋白增敏剂,授权号:ZL201010608978.8

5. 名称:一种增强多肽药物从细胞内体逃离的蛋白载药系统及其应用,授权号:ZL201310178357.4

6. 名称:一种ssDNA-蛋白质酶法交联方法,授权号:ZL201310261812.7

7. 名称:非甲醇诱导型毕赤酵母蛋白表达系统及其用途,公开号:CN101104859

8.名称:抗肿瘤药物TAT(m)-Survivin(T34A)的纯化复性方法,公开号:CN101139388

9.名称:生产二羟基丙酮的基因工程菌构建方法及其用途,公开号:CN 101092604

10. 名称:重组人甲状旁腺激素1-84的制备方法,公开号:CN1861790

11. 名称:一种静息细胞转化甘油生产1,3-丙二醇的方法,公开号:CN 101157939;

12. 名称:生产抗癌药物TAT(m)-Survivin(T34A)的工程菌的发酵放大工艺,公开号:CN101157949;

13.名称: 一种抗肿瘤蛋白质药物的质量稳定工艺,公开号:CN102366628A;

14.名称:一种肿瘤化疗药物的蛋白增敏剂,公开号:CN102366627A;

15.名称:单壁碳纳米管转载抗肿瘤药物技术及其应用,公开号:CN102370988A;

16.名称:纳米材料固载蛋白酶分子的方法及应用,公开号:CN102373192A;

17.名称:一种survivin及其突变体转基因酵母系统及其应用,公开号: CN102367422A;

18.名称:一种内含肽介导的类弹性蛋白纯化重组蛋白的方法,公开号:CN102373234A;

19.名称:一种疫苗粘膜免疫佐剂分子的制备方法,公开号:CN 102382853A

20.名称:来自LT亚基的细胞穿膜肽LTA2作为细胞内药物运输载体的应用,申请号:201410785006.4

21.名称:一种通过体外装配高效制备疫苗佐剂分子的新方法,申请号:201410843982.0

22.名称:新一代Pro-A+G多糖亲和介质及抗体蛋白药物纯化技术,申请号:20151082347.1

23.名称:一种多抗原通用流感疫苗及制备方法,申请号:201610101482.9

24.名称:一种基于组合自剪切与蛋白支架的生物活性小肽制备方法,申请号:201611243905.7

25.名称:靶向survivin纳米抗体及其制备方法和应用,申请号:201611243867.5

26.名称:一种包载重组抗肿瘤蛋白TmSm的mPEG-PLGA纳米颗粒及其制备方法和应用,申请号:201611244413.X


联系方式

联系电话:021-64250135(O)

E-mail:  maxy@ecust.edu.cnmaxingyuan@hotmail.com

通讯地址:生物反应器工程国家重点实验室 283#   邮编 200237

上海市徐汇区梅陇路130号 华东理工大学 实验十八楼1413